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Dexamethasone Up-Regulates Cadherin Expression and Cohesion of HT-1080 Human Fibrosarcoma Cells¹

Department of Molecular Biology, Princeton University, Princeton 08544 [R. A. F., J. E. S., M. S. S], and Department of Surgery, Robert Wood Johnson Medical School, New Brunswick 08903 [S. A. C.] New Jersey

The synthetic glucocorticoid dexamethasone markedly decreases the invasiveness of HT-1080 human fibrosarcoma cells. We show here that dexamethasone treatment of HT-1080 cell aggregates more than doubles their cohesivity from 3.9 to 9.7 dyne/cm. Western blot analysis shows a corresponding increase in cadherin expression. This was accompanied by an increase in the rate of calcium-dependent aggregation. Dexamethasone-treated aggregates spread to form a monolayer in Matrigel spreading assays, but the cells remained much more contiguous than their untreated counterparts. Invasion-suppression by dexamethasone may therefore be due, at least in part, to a previously unsuspected increase in cadherin-mediated cohesion.

¹ This work was supported by NIH Grant RO1 52009 from the National Institute of General Medical Sciences.

² To whom requests for reprinsts should be addressed, at Department of Surgery, Robert Wood Johnson Medical School, Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, NJ 08901. Fax: (732) 235-7493; E-mail: fotyra@rwja.umdnj.edu.

Received 3/ 4/98. Accepted 6/12/98.

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