| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Departments of Radiation Oncology [D. N., T. G., K. T., K. V. H.], Urology [G. G. H.], and Pathology [C. P., D. J. G., K. V. H.], Wayne State University School of Medicine, Detroit, Michigan 48202
Previously, we found a positive correlation between the expression of platelet-type 12-lipoxygenase (12-LOX) and the progression of human prostate adenocarcinoma (PCa; Gao et al., Urology, 46: 227–237, 1995). To determine the role of 12-LOX in PCa progression, we generated stable 12-LOX-transfected PC3 cells, which synthesize high levels of 12-LOX protein and 12(S)-hydroxyeicosatetraenoic acid metabolite. In vitro, 12-LOX-transfected PC3 cells demonstrated a proliferation rate similar to neo controls. However, following s.c. injection into athymic nude mice, 12-LOX-transfected PC3 cells formed larger tumors than did the controls. Decreased necrosis and increased vascularization were observed in the tumors from 12-LOX-transfected PC3 cells. Both endothelial cell migration and Matrigel implantation assays indicate that 12-LOX-transfected PC3 cells were more angiogenic than their neo controls. These data indicate that 12-LOX stimulates human PCa tumor growth by a novel angiogenic mechanism.
1 This work was supported by NIH CA 29997, United States Army Prostate Cancer Research Program DAMD 17-98-1-8502, and the Harper Hospital Development Fund (to K. V. H.), D. N. was supported by a fellowship from the Cancer Research Foundation of American.
2 To whom requests for reprints should be addressed, at Department of Radiation Oncology, 431 Chemistry Building, Wayne State University, Detroit, MI 48202. Phone: (313) 577-1018; Fax: (313) 577-0798.
Received 5/20/98. Accepted 7/30/98.
This article has been cited by other articles:
|
|
 |
|
  S. Sukumaran, K. B. Pittman, W. K. Patterson, J. Dickson, S. Yeend, A. Townsend, V. Broadbridge, and T. J. Price A phase I study to determine the safety, tolerability and maximum tolerated dose of green-lipped mussel (Perna canaliculus) lipid extract, in patients with advanced prostate and breast cancer Ann. Onc., October 21, 2009; (2009) mdp420v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.-Y. Kim, J.-W. Lee, S.-H. Cho, J.-M. Seo, and J.-H. Kim Role of the Low-Affinity Leukotriene B4 Receptor BLT2 in VEGF-Induced Angiogenesis Arterioscler Thromb Vasc Biol, June 1, 2009; 29(6): 915 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Assinder, Q. Dong, H. Mangs, and D. R. Richardson Pharmacological Targeting of the Integrated Protein Kinase B, Phosphatase and Tensin Homolog Deleted on Chromosome 10, and Transforming Growth Factor-{beta} Pathways in Prostate Cancer Mol. Pharmacol., March 1, 2009; 75(3): 429 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. I. Patel, J. Singh, M. Niknami, C. Kurek, M. Yao, S. Lu, F. Maclean, N. J.C. King, M. H. Gelb, K. F. Scott, et al. Cytosolic Phospholipase A2-{alpha}: A Potential Therapeutic Target for Prostate Cancer Clin. Cancer Res., December 15, 2008; 14(24): 8070 - 8079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Jin, Y. Lho, L. Connelly, Y. Wang, X. Yu, L. Saint Jean, T. C. Case, K. Ellwood-Yen, C. L. Sawyers, N. A. Bhowmick, et al. The Nuclear Factor-{kappa}B Pathway Controls the Progression of Prostate Cancer to Androgen-Independent Growth Cancer Res., August 15, 2008; 68(16): 6762 - 6769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kobayashi, R. J. Barnard, S. M. Henning, D. Elashoff, S. T. Reddy, P. Cohen, P. Leung, J. Hong-Gonzalez, S. J. Freedland, J. Said, et al. Effect of Altering Dietary {omega}-6/{omega}-3 Fatty Acid Ratios on Prostate Cancer Membrane Composition, Cyclooxygenase-2, and Prostaglandin E2 Clin. Cancer Res., August 1, 2006; 12(15): 4662 - 4670. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Nie, S. Krishnamoorthy, R. Jin, K. Tang, Y. Chen, Y. Qiao, A. Zacharek, Y. Guo, J. Milanini, G. Pages, et al. Mechanisms Regulating Tumor Angiogenesis by 12-Lipoxygenase in Prostate Cancer Cells J. Biol. Chem., July 7, 2006; 281(27): 18601 - 18609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Jankun, A. M. Aleem, S. Malgorzewicz, M. Szkudlarek, M. I. Zavodszky, D. L. DeWitt, M. Feig, S. H. Selman, and E. Skrzypczak-Jankun Synthetic curcuminoids modulate the arachidonic acid metabolism of human platelet 12-lipoxygenase and reduce sprout formation of human endothelial cells Mol. Cancer Ther., May 1, 2006; 5(5): 1371 - 1382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhang, H. Cao, and G. N. Rao Fibroblast Growth Factor-2 Is a Downstream Mediator of Phosphatidylinositol 3-Kinase-Akt Signaling in 14,15-Epoxyeicosatrienoic Acid-induced Angiogenesis J. Biol. Chem., January 13, 2006; 281(2): 905 - 914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zhang, H. Cao, and G. N. Rao 15(S)-Hydroxyeicosatetraenoic Acid Induces Angiogenesis via Activation of PI3K-Akt-mTOR-S6K1 Signaling Cancer Res., August 15, 2005; 65(16): 7283 - 7291. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. ROMANO and J. CLARIA Cyclooxygenase-2 and 5-lipoxygenase converging functions on cell proliferation and tumor angiogenesis: implications for cancer therapy FASEB J, November 1, 2003; 17(14): 1986 - 1995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Pidgeon, K. Tang, Y. L. Cai, E. Piasentin, and K. V. Honn Overexpression of Platelet-type 12-Lipoxygenase Promotes Tumor Cell Survival by Enhancing {alpha}v{beta}3 and {alpha}v{beta}5 Integrin Expression Cancer Res., July 15, 2003; 63(14): 4258 - 4267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Turgeon, S. Chouinard, P. Belanger, S. Picard, J.-F. Labbe, P. Borgeat, and A. Belanger Glucuronidation of arachidonic and linoleic acid metabolites by human UDP-glucuronosyltransferases J. Lipid Res., June 1, 2003; 44(6): 1182 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Shappell, S. J. Olson, S. E. Hannah, S. Manning, R. L. Roberts, N. Masumori, M. Jisaka, W. E. Boeglin, V. Vader, D. S. Dave, et al. Elevated Expression of 12/15-Lipoxygenase and Cyclooxygenase-2 in a Transgenic Mouse Model of Prostate Carcinoma Cancer Res., May 1, 2003; 63(9): 2256 - 2267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Aguirre-Ghiso, Y. Estrada, D. Liu, and L. Ossowski ERKMAPK Activity as a Determinant of Tumor Growth and Dormancy; Regulation by p38SAPK Cancer Res., April 1, 2003; 63(7): 1684 - 1695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Muller, M. Siebert, M. Heidt, F. Marks, P. Krieg, and G. Furstenberger Modulation of Epidermal Tumor Development Caused by Targeted Overexpression of Epidermis-type 12S-Lipoxygenase Cancer Res., August 15, 2002; 62(16): 4610 - 4616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Graham. P. Pidgeon, M. Kandouz, A. Meram, and K. V. Honn Mechanisms Controlling Cell Cycle Arrest and Induction of Apoptosis after 12-Lipoxygenase Inhibition in Prostate Cancer Cells Cancer Res., May 1, 2002; 62(9): 2721 - 2727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Wallace Nutritional and Botanical Modulation of the Inflammatory Cascade--Eicosanoids, Cyclooxygenases, and Lipoxygenases-- As an Adjunct in Cancer Therapy Integr Cancer Ther, March 1, 2002; 1(1): 7 - 37. [Abstract] [PDF]
|
|
|
|
 |
|
 U. P. Kelavkar, J. B. Nixon, C. Cohen, D. Dillehay, T. E. Eling, and K. F. Badr Overexpression of 15-lipoxygenase-1 in PC-3 human prostate cancer cells increases tumorigenesis Carcinogenesis, November 1, 2001; 22(11): 1765 - 1773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Y. Wong, W. P. Wang, C. H. Cho, X. M. Fan, M. C. M. Lin, H. F. Kung, and S. K. Lam 12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells Carcinogenesis, September 1, 2001; 22(9): 1349 - 1354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Shureiqi and S. M. Lippman Lipoxygenase Modulation to Reverse Carcinogenesis Cancer Res., September 1, 2001; 61(17): 6307 - 6312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Poster Abstracts J. Nutr., January 1, 2001; 131(1): 191S - 199. [Full Text]
|
|
|
|
|
|
U. P.Kelavkar, C. Cohen, H. Kamitani, T. E.Eling, and K. F.Badr Concordant induction of 15-lipoxygenase-1 and mutant p53 expression in human prostate adenocarcinoma: correlation with Gleason staging Carcinogenesis, October 1, 2000; 21(10): 1777 - 1787. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Nie, K. Tang, C. Diglio, and K. V. Honn Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase Blood, April 1, 2000; 95(7): 2304 - 2311. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Shappell, W. E. Boeglin, S. J. Olson, S. Kasper, and A. R. Brash 15-Lipoxygenase-2 (15-LOX-2) Is Expressed in Benign Prostatic Epithelium and Reduced in Prostate Adenocarcinoma Am. J. Pathol., July 1, 1999; 155(1): 235 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Szekeres, K. Tang, M. Trikha, and K. V. Honn Eicosanoid Activation of Extracellular Signal-regulated Kinase1/2 in Human Epidermoid Carcinoma Cells J. Biol. Chem., December 1, 2000; 275(49): 38831 - 38841. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
