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Multiplex Reverse Transcription Polymerase Chain Reaction Assessment of Sialyltransferase Expression in Human Breast Cancer¹

Laboratoire de Chimie Biologique, Unité Mixte de Recherche du C.N.R.S. n^o111, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq, France [M-A. R., A. H-L., P. D.], and Laboratoire d'Oncologie Moléculaire Humaine, Centre Oscar Lambret, BP 307, F-59020 Lille Cédex, France [M. H., L. H., J-P. P.]

Increased sialylation, especially involving the Sialyl-Lewis^a and Sialyl-Lewis^X determinants, has been reported in breast cancer. A multiplex reverse transcription-PCR method was used here to determine the expression of five sialyltransferases (ST3Gal III, ST6Gal I, ST3Gal IV, ST3Gal I, and ST3Gal II) in 49 patients surgically treated for locoregional breast cancer. We assessed the relationship between these expressions and clinical, pathological, and biological features. The most expressed sialyltransferase was ST3Gal III, which is involved in Sialyl-Lewis^a synthesis. ST3Gal III expression was positively correlated to ST6Gal I and ST3Gal IV expressions, to tumor size, and to the number of involved axillary nodes. Patients with high ST3Gal III expression had a shorter overall survival. High ST6Gal I expression was associated with histoprognostic grade III. ST6Gal I expression was negatively correlated to expression of progesterone receptor. In conclusion, high ST3Gal III and ST6Gal I expressions in human breast tumors are associated with poor prognosis markers.

¹ Supported by grants from the "Ligue Nationale Contre le Cancer" (Paris, France). M-A. R. has a fellowship of the "Association pour la Recherche contre le Cancer."

² These authors have contributed equally to this work.

³ To whom requests for reprints should be addressed, at Chimie Biologique, Université des Sciences, F-59655 Villeneuve D'Ascq. Cedex, France. Phone: 33-320-43-69-23; Fax: 33-320-43-65-55; E-mail: pdelanno@chouia.univ-lillel.fr.

Received 6/12/98. Accepted 7/31/98.

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