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[Cancer Research 58, 210-214, January 15, 1998]
© 1998 American Association for Cancer Research
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Expression of Estrogen Receptor ß Messenger RNA Variant in Breast Cancer

Esteban A. Vladusic, Ann E. Hornby, Fabiana K. Guerra-Vladusic and Ruth Lupu1

Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94710

Estrogen receptor (ER) ß is expressed in a number of tissues, including the breast. We have recently shown that ER-ß mRNA is regulated by estradiol (E2) and that antiestrogens antagonize E2 induction of ER-ß mRNA. Here, we identify by reverse transcription-PCR and by the RNase protection assay a mRNA coding for a variant of ER-ß that is coexpressed with wild-type ER-ß in the ER-{alpha}-negative, estrogen-independent breast cancer cell line MDA-MB-231 and in malignant breast tumor specimens. In contrast, this variant was not seen in the tested normal breast tissue. Sequence analysis of the ER-ß variant PCR product revealed the absence of 139 bp within the hormone-binding domain. This ER-ß deletion corresponds precisely to the entire exon 5 of ER-{alpha}. The ER-ß variant protein is predicted to lack part of the hormone-binding domain and may bind E2 with lower affinity than the wild-type ER-ß protein.

1 To whom requests for reprints should be addressed, at Lawrence Berkeley National Laboratory, University of California, One Cyclotron Road, Berkeley, CA 94720. Phone: (510) 486-6874; Fax: (510) 486-7289; E-mail: rlupu@lbl.gov.

Received 9/16/97. Accepted 12/ 2/97.




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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 1998 by the American Association for Cancer Research.