| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Genetic and Molecular Biology Program [C. S., R. C. M., M. M. S., S. G., J. O., R. F.], Department of Microbiology and Immunology [C. S., M. M. S., S. G., R. F.], and Department of Biochemistry and Molecular Pharmacology [R. C. M., J. O.], Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107
DNA mismatch repair (MMR) plays a vital role in the faithful replication of DNA, and its inactivation leads to a mutator phenotype that has been associated with the common cancer susceptibility syndrome Hereditary Non-Polyposis Colorectal Cancer (HNPCC). Here, we report on a novel human exonuclease (hExoI) that is related to the yeast exonuclease 1. The hExoI cDNA comprises 2541 bp, which code for a Mr 94,000 protein that appears to be highly expressed in testis tissue and at very low levels in other tissues. The hExoI gene has 14 exons and is located on chromosome 1q43, as determined by fluorescence in situ hybridization and radiation hybrid mapping. hExoI was found to interact strongly with the human MMR protein hMSH2, suggesting its involvement in the MMR process and/or DNA recombination.
1 This work was supported by NIH Grants CA56542 and CA67007 (to R. F.).
2 To whom requests for reprints should be addressed, at Kimmel Cancer Center BLSB933, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107. Phone: (215) 503-1346; Fax: (215) 923-1098; E-mail: cschmutt@hendrix.jci.tju.edu.
Received 8/ 5/98. Accepted 9/ 9/98.
This article has been cited by other articles:
|
|
 |
|
  Y. Zhang, F. Yuan, D. Wang, L. Gu, and G.-M. Li Identification of Regulatory Factor X as a Novel Mismatch Repair Stimulatory Factor J. Biol. Chem., May 9, 2008; 283(19): 12730 - 12735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. El-Shemerly, D. Hess, A. K. Pyakurel, S. Moselhy, and S. Ferrari ATR-dependent pathways control hEXO1 stability in response to stalled forks Nucleic Acids Res., February 2, 2008; 36(2): 511 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. O. Knudsen, F. C. Nielsen, L. Vinther, R. Bertelsen, S. Holten-Andersen, S. E. Liberti, R. Hofstra, K. Kooi, and L. J. Rasmussen Nuclear localization of human DNA mismatch repair protein exonuclease 1 (hEXO1) Nucleic Acids Res., April 10, 2007; (2007) gkl1166v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Wilson, A. Vaisman, S. A. Martomo, P. Sullivan, L. Lan, F. Hanaoka, A. Yasui, R. Woodgate, and P. J. Gearhart MSH2-MSH6 stimulates DNA polymerase {eta}, suggesting a role for A:T mutations in antibody genes J. Exp. Med., February 22, 2005; 201(4): 637 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Meyers, M. W. Wagner, A. Mazurek, C. Schmutte, R. Fishel, and D. A. Boothman DNA Mismatch Repair-dependent Response to Fluoropyrimidine-generated Damage J. Biol. Chem., February 18, 2005; 280(7): 5516 - 5526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yamamoto, H. Hanafusa, M. Ouchida, M. Yano, H. Suzuki, M. Murakami, M. Aoe, N. Shimizu, K. Nakachi, and K. Shimizu Single nucleotide polymorphisms in the EXO1 gene and risk of colorectal cancer in a Japanese population Carcinogenesis, February 1, 2005; 26(2): 411 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Liberti and L. J. Rasmussen Is hEXO1 a Cancer Predisposing Gene? Mol. Cancer Res., August 1, 2004; 2(8): 427 - 432. [Full Text] [PDF]
|
|
|
|
|
|
K. S. Shim, C. Schmutte, G. Tombline, C. D. Heinen, and R. Fishel hXRCC2 Enhances ADP/ATP Processing and Strand Exchange by hRAD51 J. Biol. Chem., July 16, 2004; 279(29): 30385 - 30394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Martomo, W. W. Yang, and P. J. Gearhart A Role for Msh6 But Not Msh3 in Somatic Hypermutation and Class Switch Recombination J. Exp. Med., July 6, 2004; 200(1): 61 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Yuan, L. Gu, S. Guo, C. Wang, and G.-M. Li Evidence for Involvement of HMGB1 Protein in Human DNA Mismatch Repair J. Biol. Chem., May 14, 2004; 279(20): 20935 - 20940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Wei, A. B. Clark, E. Wong, M. F. Kane, D. J. Mazur, T. Parris, N. K. Kolas, R. Russell, H. Hou Jr., B. Kneitz, et al. Inactivation of Exonuclease 1 in mice results in DNA mismatch repair defects, increased cancer susceptibility, and male and female sterility Genes & Dev., March 1, 2003; 17(5): 603 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Sun, L. Zheng, and B. Shen Functional Alterations of Human Exonuclease 1 Mutants Identified in Atypical Hereditary Nonpolyposis Colorectal Cancer Syndrome Cancer Res., November 1, 2002; 62(21): 6026 - 6030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Miturski, M. Bogusiewicz, C. Ciotta, M. Bignami, M. Gogacz, and D. Burnouf Mismatch Repair Genes and Microsatellite Instability as Molecular Markers for Gynecological Cancer Detection Experimental Biology and Medicine, September 1, 2002; 227(8): 579 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gu, J. Wu, B.-B. Zhu, and G.-M. Li Deficiency of a novel mismatch repair activity in a bladder tumor cell line Nucleic Acids Res., July 1, 2002; 30(13): 2758 - 2763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Tomer, A. B. Buermeyer, M. M. Nguyen, and R. M. Liskay Contribution of Human Mlh1 and Pms2 ATPase Activities to DNA Mismatch Repair J. Biol. Chem., June 7, 2002; 277(24): 21801 - 21809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Genschel, L. R. Bazemore, and P. Modrich Human Exonuclease I Is Required for 5' and 3' Mismatch Repair J. Biol. Chem., April 5, 2002; 277(15): 13302 - 13311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-i. Lee, L. H. Nguyen, D. Barsky, M. Fernandes, and D. M. Wilson III Molecular interactions of human Exo1 with DNA Nucleic Acids Res., February 15, 2002; 30(4): 942 - 949. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Mansour, C. Tornier, E. Lehmann, M. Darmon, and O. Fleck Control of GT Repeat Stability in Schizosaccharomyces pombe by Mismatch Repair Factors Genetics, May 1, 2001; 158(1): 77 - 85. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. E. Yoder and F. D. Bushman Repair of Gaps in Retroviral DNA Integration Intermediates J. Virol., December 1, 2000; 74(23): 11191 - 11200. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Evans and E. Alani Roles for Mismatch Repair Factors in Regulating Genetic Recombination Mol. Cell. Biol., November 1, 2000; 20(21): 7839 - 7844. [Full Text]
|
|
|
|
|
|
B.-I. Lee and D. M. Wilson III The RAD2 Domain of Human Exonuclease 1 Exhibits 5' to 3' Exonuclease and Flap Structure-specific Endonuclease Activities J. Biol. Chem., December 31, 1999; 274(53): 37763 - 37769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Qiu, Y. Qian, V. Chen, M.-X. Guan, and B. Shen Human Exonuclease 1 Functionally Complements Its Yeast Homologues in DNA Recombination, RNA Primer Removal, and Mutation Avoidance J. Biol. Chem., June 18, 1999; 274(25): 17893 - 17900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Schmutte, M. M. Sadoff, K.-S. Shim, S. Acharya, and R. Fishel The Interaction of DNA Mismatch Repair Proteins with Human Exonuclease I J. Biol. Chem., August 31, 2001; 276(35): 33011 - 33018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Matton, J. Simonetti, and K. Williams Identification of Mismatch Repair Protein Complexes in HeLa Nuclear Extracts and Their Interaction with Heteroduplex DNA J. Biol. Chem., June 2, 2000; 275(23): 17808 - 17813. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
