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Characterization of Alkaline Phosphatase Genes Expressed in Seminoma by cDNA Cloning

Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa 259-1193 [A. S., A. A., T. B., H. I.]; Natural Products Research Department, Teikoku Hormon Manufacturing Co., Ltd., Kawasaki 213-0033 [T. Y.]; and Department of Urology, Osaka Medical College, Osaka 569 [Y. K.], Japan

Two members of a placental alkaline phosphatase (PLAP) family, PLAP and PLAP-like or germ cell alkaline phosphatase, are aberrantly expressed in tumors of ecotropic origin. To characterize alkaline phosphatase induced in seminoma, alkaline phosphatase cDNA clones were isolated from a cDNA library constructed from seminoma cells and characterized by nucleotide sequence determination. Thus, isolated cDNA clones were classified into two types, germ cell alkaline phosphatase (PLAP-like) and liver/bone/kidney-type alkaline phosphatase (L/B/K AP). These results suggest that other than the PLAP family members, the expression of L/B/K AP is enhanced in seminoma and can serve as a tumor marker in seminoma.

¹ To whom requests for reprints should be addressed, at Department of Molecular Life Science, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-11, Japan. Phone: 81-463-93-1121 (extension 2312); Fax: 81-463-94-8884.

Received 7/27/98. Accepted 10/ 1/98.