Cancer Research Meeting Calendar Genetics and Biology of Brain Cancer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
[Cancer Research 58, 5089-5096, November 15, 1998]
© 1998 American Association for Cancer Research
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, R.-P.
Right arrow Articles by Boynton, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, R.-P.
Right arrow Articles by Boynton, A. L.

Reversion of the Neoplastic Phenotype of Human Glioblastoma Cells by Connexin 43 (cx43)1

Ruo-Pan Huang2, Yan Fan, Mohammad Z. Hossain, Ao Peng, Zi-Li Zeng and Alton L. Boynton

Molecular Medicine, Northwest Hospital, Seattle, Washington 98125 [R-P. H, Y. F., M. Z. H., A. P., Z-L. Z., A. L. B.], and Shantou University Medical College, Shantou City, Guangdong Province, People's Republic of China, 51503 [Z-L. Z.]

Connexins (cx), structural components of gap junction, are believed to play a role in the regulation of cell proliferation and suppression of the neoplastic phenotype. We used human brain glioblastoma tumor cells as a model system to test this hypothesis. Western blot and reverse transcription-PCR analysis indicate that the expression levels of the gap junction protein connexin 43 (cx43) are profoundly decreased in several human brain tumor cell lines examined. Transfection of human cx43 into human glioblastoma cell lines U251 and T98G profoundly reduces cell proliferation in monolayer culture, in soft agar, and in athymic nude mice. Surprisingly, these effects are not associated with the establishment of gap junction communication in cx43 transfected cells. We conclude that the loss of cx43 expression may play a role in the development of human gliomas and that cx43 acts as a tumor suppressor gene to human glioblastoma.

1 This work was supported by NIH Grants CA39745 and CA57064 (both to A. L. B.).

2 To whom requests for reprints should be addressed, at Molecular Medicine, Northwest Hospital, 120 Northgate Plaza, Suite 230, Seattle, WA 98125. Phone: (206) 368-3063; Fax: (206) 368-3009; E-mail: rphuang@nwlink.com or rhuang@nwhsea.org.

Received 4/29/98. Accepted 9/17/98.




This article has been cited by other articles:


Home page
 Cancer Res.Home page
E. Peterson-Roth, C. M. Brdlik, and P. M. Glazer
Src-Induced Cisplatin Resistance Mediated by Cell-to-Cell Communication
Cancer Res., April 15, 2009; 69(8): 3619 - 3624.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Ruiz-Meana, A. Rodriguez-Sinovas, A. Cabestrero, K. Boengler, G. Heusch, and D. Garcia-Dorado
Mitochondrial connexin43 as a new player in the pathophysiology of myocardial ischaemia-reperfusion injury
Cardiovasc Res, January 15, 2008; 77(2): 325 - 333.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. A. Banks, X. S. Yu, Q. Shi, and J. X. Jiang
Promotion of lens epithelial-fiber differentiation by the C-terminus of connexin 45.6 a role independent of gap junction communication
J. Cell Sci., October 15, 2007; 120(20): 3602 - 3612.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Shen, P. R. Khusial, X. Li, H. Ichikawa, A. P. Moreno, and G. S. Goldberg
Src Utilizes Cas to Block Gap Junctional Communication Mediated by Connexin43
J. Biol. Chem., June 29, 2007; 282(26): 18914 - 18921.
[Abstract] [Full Text] [PDF]

Home page
 Br J OphthalmolHome page
V. A Shanmuganathan, T. Foster, B. B Kulkarni, A. Hopkinson, T. Gray, D. G Powe, J. Lowe, and H. S Dua
Morphological characteristics of the limbal epithelial crypt
Br J Ophthalmol, April 1, 2007; 91(4): 514 - 519.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
C. M.J. Conklin, J. F. Bechberger, D. MacFabe, N. Guthrie, E. M. Kurowska, and C. C. Naus
Genistein and quercetin increase connexin43 and suppress growth of breast cancer cells
Carcinogenesis, January 1, 2007; 28(1): 93 - 100.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Squecco, C. Sassoli, F. Nuti, M. Martinesi, F. Chellini, D. Nosi, S. Zecchi-Orlandini, F. Francini, L. Formigli, and E. Meacci
Sphingosine 1-Phosphate Induces Myoblast Differentiation through Cx43 Protein Expression: A Role for a Gap Junction-dependent and -independent Function
Mol. Biol. Cell, November 1, 2006; 17(11): 4896 - 4910.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
E. McLachlan, Q. Shao, H.-l. Wang, S. Langlois, and D. W. Laird
Connexins Act as Tumor Suppressors in Three-dimensional Mammary Cell Organoids by Regulating Differentiation and Angiogenesis.
Cancer Res., October 15, 2006; 66(20): 9886 - 9894.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
K. Yu, K. Ganesan, L. D. Miller, and P. Tan
A modular analysis of breast cancer reveals a novel low-grade molecular signature in estrogen receptor-positive tumors.
Clin. Cancer Res., June 1, 2006; 12(11): 3288 - 3296.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Q. Shao, H. Wang, E. McLachlan, G. I.L. Veitch, and D. W. Laird
Down-regulation of Cx43 by Retroviral Delivery of Small Interfering RNA Promotes an Aggressive Breast Cancer Cell Phenotype
Cancer Res., April 1, 2005; 65(7): 2705 - 2711.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
Y. Nakamura, C.-C. Chang, T. Mori, K. Sato, K. Ohtsuki, B. L. Upham, and J. E. Trosko
Augmentation of differentiation and gap junction function by kaempferol in partially differentiated colon cancer cells
Carcinogenesis, March 1, 2005; 26(3): 665 - 671.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Leithe and E. Rivedal
Ubiquitination and Down-regulation of Gap Junction Protein Connexin-43 in Response to 12-O-Tetradecanoylphorbol 13-Acetate Treatment
J. Biol. Chem., November 26, 2004; 279(48): 50089 - 50096.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Fiorini, B. Mograbi, L. Cronier, I. Bourget, X. Decrouy, M. Nebout, B. Ferrua, A. Malassine, M. Samson, P. Fenichel, et al.
Dominant negative effect of connexin33 on gap junctional communication is mediated by connexin43 sequestration
J. Cell Sci., September 15, 2004; 117(20): 4665 - 4672.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. T. Fu, J. F. Bechberger, M. A. Ozog, B. Perbal, and C. C. Naus
CCN3 (NOV) Interacts with Connexin43 in C6 Glioma Cells: POSSIBLE MECHANISM OF CONNEXIN-MEDIATED GROWTH SUPPRESSION
J. Biol. Chem., August 27, 2004; 279(35): 36943 - 36950.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Leithe and E. Rivedal
Epidermal growth factor regulates ubiquitination, internalization and proteasome-dependent degradation of connexin43
J. Cell Sci., March 1, 2004; 117(7): 1211 - 1220.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. B. Alexander, H. Ichikawa, J. F. Bechberger, V. Valiunas, M. Ohki, C. C. G. Naus, T. Kunimoto, H. Tsuda, W. T. Miller, and G. S. Goldberg
Normal Cells Control the Growth of Neighboring Transformed Cells Independent of Gap Junctional Communication and Src Activity
Cancer Res., February 15, 2004; 64(4): 1347 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-W. Zhang, M. Kaneda, and I. Morita
The Gap Junction-independent Tumor-suppressing Effect of Connexin 43
J. Biol. Chem., November 7, 2003; 278(45): 44852 - 44856.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J.-T. Chen, Y.-W. Cheng, M.-C. Chou, T. Sen-Lin, W.-W. Lai, W. L. Ho, and H. Lee
The Correlation between Aberrant Connexin 43 mRNA Expression Induced by Promoter Methylation and Nodal Micrometastasis in Non-Small Cell Lung Cancer
Clin. Cancer Res., September 15, 2003; 9(11): 4200 - 4204.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
B. Mograbi, E. Corcelle, N. Defamie, M. Samson, M. Nebout, D. Segretain, P. Fenichel, and G. Pointis
Aberrant Connexin 43 endocytosis by the carcinogen lindane involves activation of the ERK/mitogen-activated protein kinase pathway
Carcinogenesis, August 1, 2003; 24(8): 1415 - 1423.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. P. Stains, F. Lecanda, J. Screen, D. A. Towler, and R. Civitelli
Gap Junctional Communication Modulates Gene Transcription by Altering the Recruitment of Sp1 and Sp3 to Connexin-response Elements in Osteoblast Promoters
J. Biol. Chem., June 27, 2003; 278(27): 24377 - 24387.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Y.-W. Zhang, K. Nakayama, K.-I. Nakayama, and I. Morita
A Novel Route for Connexin 43 to Inhibit Cell Proliferation: Negative Regulation of S-Phase Kinase-associated Protein (Skp 2)
Cancer Res., April 1, 2003; 63(7): 1623 - 1630.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. H.-C. Lin, J. Yang, S. Liu, T. Takano, X. Wang, Q. Gao, K. Willecke, and M. Nedergaard
Connexin Mediates Gap Junction-Independent Resistance to Cellular Injury
J. Neurosci., January 15, 2003; 23(2): 430 - 441.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Qin, Q. Shao, H. Curtis, J. Galipeau, D. J. Belliveau, T. Wang, M. A. Alaoui-Jamali, and D. W. Laird
Retroviral Delivery of Connexin Genes to Human Breast Tumor Cells Inhibits in Vivo Tumor Growth by a Mechanism That Is Independent of Significant Gap Junctional Intercellular Communication
J. Biol. Chem., August 2, 2002; 277(32): 29132 - 29138.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. A. Ozog, J. F. Bechberger, and C. C. G. Naus
Ciliary Neurotrophic Factor (CNTF) in Combination with Its Soluble Receptor (CNTFR{alpha}) Increases Connexin43 Expression and Suppresses Growth of C6 Glioma Cells
Cancer Res., June 1, 2002; 62(12): 3544 - 3548.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. Li, S. Brodsky, S. Kumari, V. Valiunas, P. Brink, J.-I. Kaide, A. Nasjletti, and M. S. Goligorsky
Paradoxical overexpression and translocation of connexin43 in homocysteine-treated endothelial cells
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2124 - H2133.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Huang, Y. Lin, C. C. Wang, J. Gano, B. Lin, Q. Shi, A. Boynton, J. Burke, and R.-P. Huang
Connexin 43 Suppresses Human Glioblastoma Cell Growth by Down-Regulation of Monocyte Chemotactic Protein 1, as Discovered Using Protein Array Technology
Cancer Res., May 1, 2002; 62(10): 2806 - 2812.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
A. Muramatsu, M. Iwai, T. Morikawa, S. Tanaka, T. Mori, Y. Harada, and T. Okanoue
Influence of transfection with connexin 26 gene on malignant potential of human hepatoma cells
Carcinogenesis, February 1, 2002; 23(2): 351 - 358.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
G. Li, K. Satyamoorthy, and M. Herlyn
DYNAMICS OF CELL INTERACTIONS AND COMMUNICATIONS DURING MELANOMA DEVELOPMENT
Critical Reviews in Oral Biology & Medicine, January 1, 2002; 13(1): 62 - 70.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
F. Princen, P. Robe, D. Gros, T. Jarry-Guichard, J. Gielen, M.-P. Merville, and V. Bours
Rat gap junction connexin-30 inhibits proliferation of glioma cell lines
Carcinogenesis, March 1, 2001; 22(3): 507 - 513.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. S. Goldberg, J. F. Bechberger, Y. Tajima, M. Merritt, Y. Omori, M. A. Gawinowicz, R. Narayanan, Y. Tan, Y. Sanai, H. Yamasaki, et al.
Connexin43 Suppresses MFG-E8 While Inducing Contact Growth Inhibition of Glioma Cells
Cancer Res., November 1, 2000; 60(21): 6018 - 6026.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
M. Mesnil and H. Yamasaki
Bystander Effect in Herpes Simplex Virus-Thymidine Kinase/Ganciclovir Cancer Gene Therapy: Role of Gap-junctional Intercellular Communication1
Cancer Res., August 1, 2000; 60(15): 3989 - 3999.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
M Hsu, T Andl, G Li, J. Meinkoth, and M Herlyn
Cadherin repertoire determines partner-specific gap junctional communication during melanoma progression
J. Cell Sci., January 5, 2000; 113(9): 1535 - 1542.
[Abstract] [PDF]

Home page
 CarcinogenesisHome page
R.-P. Huang, A. Peng, M. Z. Hossain, Y. Fan, A. Jagdale, and A. L. Boynton
Tumor promotion by hydrogen peroxide in rat liver epithelial cells
Carcinogenesis, March 1, 1999; 20(3): 485 - 492.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
H. Li, S. Brodsky, S. Kumari, V. Valiunas, P. Brink, J.-I. Kaide, A. Nasjletti, and M. S. Goligorsky
Paradoxical overexpression and translocation of connexin43 in homocysteine-treated endothelial cells
Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2124 - H2133.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 1998 by the American Association for Cancer Research.