.JPG?ad=18503&adview=true)
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Cellular Defense and Carcinogenesis Section, Basic Research Laboratory, Division of Basic Sciences [H. P. C., G. C. Y.] and Intramural Research Support Program, SAIC Frederick [P. J. D.], National Cancer Institute-Frederick Cancer Research and Development Center, NIH, Frederick, Maryland 21702-1201
Resveratrol, a compound present in a variety of plants, was recently shown to have potent chemopreventive activity against aryl hydrocarbon-induced tumorigenesis in mice. Therefore, in the present study, we examined the effect of resveratrol on the function of the aryl hydrocarbon receptor (AHR) and the transcription of CYP1A1 in human HepG2 hepatoma cells. Resveratrol inhibited the increase in cytochrome P450 (CYP) 1A1 mRNA caused by the AHR ligand 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a concentration-dependent manner. The induction of transcription of an aryl hydrocarbon-responsive reporter vector containing the CYP1A1 promoter by TCDD was likewise inhibited by resveratrol. Resveratrol also inhibited the constitutive level of CYP1A1 mRNA and reporter vector transcription in HepG2 cells. The increase in CYP1A1 enzyme activity induced by TCDD was inhibited by resveratrol. Resveratrol prevented the TCDD-induced transformation of the cytosolic AHR to its nuclear DNA-binding form. However, resveratrol had no effect on the binding of TCDD to the cytosolic AHR. These data demonstrate that resveratrol inhibits CYP1A1 expression in vitro, and that it does this by preventing the binding of the AHR to promoter sequences that regulate CYP1A1 transcription. This activity may be an important part of the chemopreventive activity of resveratrol.
1 Supported in part by federal funds from the National Cancer Institute, NIH, under Contract No. NO1-CO-56000.
2 To whom requests for reprints should be addressed, at Basic Research Laboratory, National Cancer Institute-Frederick Cancer Research and Development Center, Building 560/Room 12-05, P. O. Box B, Frederick, MD 21702-1201. Phone: (301) 846-5160; Fax: (301) 846-6093; E-mail: hciolino@mail.ncifcrf.gov.
Received 7/ 8/98. Accepted 10/15/98.
This article has been cited by other articles:
|
|
 |
|
  S. A. Gatz and L. Wiesmuller Take a break--resveratrol in action on DNA Carcinogenesis, February 1, 2008; 29(2): 321 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sienkiewicz, H. P. Ciolino, B. J. Leslie, P. J. Hergenrother, K. Singletary, and G. C. Yeh A novel synthetic analogue of a constituent of Isodon excisus inhibits transcription of CYP1A1, -1A2 and -1B1 by preventing activation of the aryl hydrocarbon receptor Carcinogenesis, May 1, 2007; 28(5): 1052 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ebert, A. Seidel, and A. Lampen Phytochemicals Induce Breast Cancer Resistance Protein in Caco-2 Cells and Enhance the Transport of Benzo[a]pyrene-3-sulfate Toxicol. Sci., April 1, 2007; 96(2): 227 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Schreiber, C. Kohle, F. Buckler, S. Schmohl, A. Braeuning, A. Schmiechen, M. Schwarz, and P. A. Munzel REGULATION OF CYP1A1 GENE EXPRESSION BY THE ANTIOXIDANT TERT-BUTYLHYDROQUINONE Drug Metab. Dispos., July 1, 2006; 34(7): 1096 - 1101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Petersen, S. Krishnan, and E. D. Hudgens The Aryl Hydrocarbon Receptor Pathway and Sexual Differentiation of Neuroendocrine Functions Endocrinology, June 1, 2006; 147(6): s33 - s42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Aluru and M. M. Vijayan Aryl Hydrocarbon Receptor Activation Impairs Cortisol Response to Stress in Rainbow Trout by Disrupting the Rate-Limiting Steps in Steroidogenesis Endocrinology, April 1, 2006; 147(4): 1895 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Chun, S.-K. Lee, and M. Y. Kim MODULATION OF HUMAN CYTOCHROME P450 1B1 EXPRESSION BY 2,4,3',5'-TETRAMETHOXYSTILBENE Drug Metab. Dispos., December 1, 2005; 33(12): 1771 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wen and T. Walle Preferential induction of CYP1B1 by benzo[a]pyrene in human oral epithelial cells: impact on DNA adduct formation and prevention by polyphenols Carcinogenesis, October 1, 2005; 26(10): 1774 - 1781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wen, U.K. Walle, and T. Walle 5,7-Dimethoxyflavone downregulates CYP1A1 expression and benzo[a]pyrene-induced DNA binding in Hep G2 cells Carcinogenesis, April 1, 2005; 26(4): 803 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-H. Chen, Y.-J. Hurh, H.-K. Na, J.-H. Kim, Y.-J. Chun, D.-H. Kim, K.-S. Kang, M.-H. Cho, and Y.-J. Surh Resveratrol inhibits TCDD-induced expression of CYP1A1 and CYP1B1 and catechol estrogen-mediated oxidative DNA damage in cultured human mammary epithelial cells Carcinogenesis, October 1, 2004; 25(10): 2005 - 2013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. PERVAIZ Resveratrol: from grapevines to mammalian biology FASEB J, November 1, 2003; 17(14): 1975 - 1985. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vernhet, N. Allain, M. Le Vee, F. Morel, A. Guillouzo, and O. Fardel Blockage of Multidrug Resistance-Associated Proteins Potentiates the Inhibitory Effects of Arsenic Trioxide on CYP1A1 Induction by Polycyclic Aromatic Hydrocarbons J. Pharmacol. Exp. Ther., January 1, 2003; 304(1): 145 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Liu, R. M. Russell, and X.-D. Wang Exposing Ferrets to Cigarette Smoke and a Pharmacological Dose of {beta}-Carotene Supplementation Enhance In Vitro Retinoic Acid Catabolism in Lungs via Induction of Cytochrome P450 Enzymes J. Nutr., January 1, 2003; 133(1): 173 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Banerjee, C. Bueso-Ramos, and B. B. Aggarwal Suppression of 7,12-Dimethylbenz(a)anthracene-induced Mammary Carcinogenesis in Rats by Resveratrol: Role of Nuclear Factor-{kappa}B, Cyclooxygenase 2, and Matrix Metalloprotease 9 Cancer Res., September 1, 2002; 62(17): 4945 - 4954. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Le Ferrec, D. Lagadic-Gossmann, C. Rauch, C. Bardiau, K. Maheo, F. Massiere, M. Le Vee, A. Guillouzo, and F. Morel Transcriptional Induction of CYP1A1 by Oltipraz in Human Caco-2 Cells Is Aryl Hydrocarbon Receptor- and Calcium-dependent J. Biol. Chem., June 28, 2002; 277(27): 24780 - 24787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Simopoulos The Mediterranean Diets: What Is So Special about the Diet of Greece? The Scientific Evidence J. Nutr., November 1, 2001; 131(11): 3065S - 3073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Allen, L. Mueller, S. N. Williams, L. C. Quattrochi, and J. Raucy The Use of a High-Volume Screening Procedure to Assess the Effects of Dietary Flavonoids on Human CYP1A1 Expression Drug Metab. Dispos., August 1, 2001; 29(8): 1074 - 1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gusman, H. Malonne, and G. Atassi A reappraisal of the potential chemopreventive and chemotherapeutic properties of resveratrol Carcinogenesis, August 1, 2001; 22(8): 1111 - 1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Nazarenko, S. D. Dertinger, and T. A. Gasiewicz In Vivo Antagonism of AhR-Mediated Gene Induction by 3'-Methoxy-4'-nitroflavone in TCDD-Responsive lacZ Mice Toxicol. Sci., June 1, 2001; 61(2): 256 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kimura and H. Okuda Resveratrol Isolated from Polygonum cuspidatum Root Prevents Tumor Growth and Metastasis to Lung and Tumor-Induced Neovascularization in Lewis Lung Carcinoma-Bearing Mice J. Nutr., June 1, 2001; 131(6): 1844 - 1849. [Abstract] [Full Text]
|
|
|
|
|
|
C. J. MacDonald, H. P. Ciolino, and G. C. Yeh Dibenzoylmethane Modulates Aryl Hydrocarbon Receptor Function and Expression of Cytochromes P450 1A1, 1A2, and 1B1 Cancer Res., May 1, 2001; 61(10): 3919 - 3924. [Abstract] [Full Text]
|
|
|
|
|
|
X. Coumoul, M. Diry, C. Robillot, and R. Barouki Differential Regulation of Cytochrome P450 1A1 and 1B1 by a Combination of Dioxin and Pesticides in the Breast Tumor Cell Line MCF-7 Cancer Res., May 1, 2001; 61(10): 3942 - 3948. [Abstract] [Full Text]
|
|
|
|
|
|
J.-E. Lee and S. Safe 3',4'-Dimethoxyflavone as an Aryl Hydrocarbon Receptor Antagonist in Human Breast Cancer Cells Toxicol. Sci., December 1, 2000; 58(2): 235 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Quadri, A. N. Qadri, M. E. Hahn, K. K. Mann, and D. H. Sherr The Bioflavonoid Galangin Blocks Aryl Hydrocarbon Receptor Activation and Polycyclic Aromatic Hydrocarbon-Induced Pre-B Cell Apoptosis Mol. Pharmacol., September 1, 2000; 58(3): 515 - 525. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. K. Manna, A. Mukhopadhyay, and B. B. Aggarwal Resveratrol Suppresses TNF-Induced Activation of Nuclear Transcription Factors NF-{kappa}B, Activator Protein-1, and Apoptosis: Potential Role of Reactive Oxygen Intermediates and Lipid Peroxidation J. Immunol., June 15, 2000; 164(12): 6509 - 6519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. P. Ciolino and G. C. Yeh The Steroid Hormone Dehydroepiandrosterone Inhibits CYP1A1 Expression in Vitro By a Post-transcriptional Mechanism J. Biol. Chem., December 3, 1999; 274(49): 35186 - 35190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. P. Ciolino and G. C. Yeh Inhibition of Aryl Hydrocarbon-Induced Cytochrome P-450 1A1 Enzyme Activity and CYP1A1 Expression by Resveratrol Mol. Pharmacol., October 1, 1999; 56(4): 760 - 767. [Abstract] [Full Text]
|
|
|
|
|
|
R. F. Casper, M. Quesne, I. M. Rogers, T. Shirota, A. Jolivet, E. Milgrom, and J.-F. Savouret Resveratrol Has Antagonist Activity on the Aryl Hydrocarbon Receptor: Implications for Prevention of Dioxin Toxicity Mol. Pharmacol., October 1, 1999; 56(4): 784 - 790. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
