.JPG?ad=18503&adview=true)
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Division of Clinical Genetics, Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Suita City, Osaka 565 [K. I., Y. N., Y. M.]; Departments of Surgery [S. N., M. K., S. Im.] and Pathology [S. Is.], Osaka Medical Center for Cancer and Cardiovascular Diseases, Higashinari-ku, Osaka City, Osaka 537; Otsuka Assay Laboratory, Otsuka Pharmaceutical Co. Ltd., Kawauchi Cho, Tokushima City, Tokushima 771-01 [M. K., T. F.]; and Laboratory of Molecular Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108 [Y. N.], Japan
Among 222 primary colorectal cancers we examined, 58 showed no detectable APC mutations by the protein truncation test. We screened those 58 tumors for somatic mutations in the ß-catenin gene. Although amino acid substitutions in serine or threonine residues in exon 3 had been reported, we found no such mutations; however, in seven tumors, we detected somatic interstitial deletions of 234–760 bp, each of which included all or part of exon 3. Short nucleotide sequences at both ends of each deletion were either identical or complementary, indicating that repeated or inversely repeated sequences were involved in the somatic rearrangements. Reverse transcription-PCR experiments using RNAs isolated from three of these seven tumors detected transcripts that lacked exon 3, in addition to the normal transcript. In one of these cases, we confirmed accumulation of aberrant ß-catenin protein in cytoplasm and nuclei of cancer cells by Western and immunohistochemical analyses. This result suggested that, in the absence of a peptide encoded by exon 3, ß-catenin is stabilized and has a dominant oncogenic effect on colorectal tumorigenesis.
1 This work was supported in part by a special grant for Strategic Advanced Research on Cancer from the Ministry of Education, Science, Sports and Culture of Japan, and Research for the Future Program Grant 96L00102 from the Japan Society for the Promotion of Science.
2 Present address: Second Department of Surgery, Osaka University Medical School, 2-2 Yamadaoka, Suita City, Osaka 565, Japan.
3 To whom requests for reprints should be addressed. Phone: 81-3-5449-5372; Fax: 81-3-5449-5433; E-mail: yusuke@ims.u-tokyo.ac.jp.
Received 9/22/97. Accepted 1/ 6/98.
This article has been cited by other articles:
|
|
 |
|
  A. Otto, C. Schmidt, G. Luke, S. Allen, P. Valasek, F. Muntoni, D. Lawrence-Watt, and K. Patel Canonical Wnt signalling induces satellite-cell proliferation during adult skeletal muscle regeneration J. Cell Sci., September 1, 2008; 121(17): 2939 - 2950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nosho, H. Yamamoto, T. Takahashi, M. Mikami, H. Taniguchi, N. Miyamoto, Y. Adachi, Y. Arimura, F. Itoh, K. Imai, et al. Genetic and epigenetic profiling in early colorectal tumors and prediction of invasive potential in pT1 (early invasive) colorectal cancers Carcinogenesis, June 1, 2007; 28(6): 1364 - 1370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Seki, H. Yamamoto, C. Yee Ngan, M. Yasui, N. Tomita, K. Kitani, I. Takemasa, M. Ikeda, M. Sekimoto, N. Matsuura, et al. Construction of a novel DNA decoy that inhibits the oncogenic {beta}-catenin/T-cell factor pathway. Mol. Cancer Ther., April 1, 2006; 5(4): 985 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Nosho, H Yamamoto, M Mikami, T Takahashi, Y Adachi, T Endo, K Hirata, K Imai, and Y Shinomura Laterally spreading tumour in which interstitial deletion of {beta}-catenin exon 3 was detected Gut, October 1, 2005; 54(10): 1504 - 1505. [Full Text] [PDF]
|
|
|
|
|
|
V Johnson, E Volikos, S E Halford, E T Eftekhar Sadat, S Popat, I Talbot, K Truninger, J Martin, J Jass, R Houlston, et al. Exon 3 {beta}-catenin mutations are specifically associated with colorectal carcinomas in hereditary non-polyposis colorectal cancer syndrome Gut, February 1, 2005; 54(2): 264 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.C. Sills, H.L. Hong, G. Flake, C. Moomaw, N. Clayton, G.A. Boorman, J. Dunnick, and T.R. Devereux o-Nitrotoluene-induced large intestinal tumors in B6C3F1 mice model human colon cancer in their molecular pathogenesis Carcinogenesis, April 1, 2004; 25(4): 605 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. C. Wong, E. S. F. Lo, K. C. Lee, J. K. C. Chan, and W. L. W. Hsiao Prognostic and Diagnostic Significance of {beta}-Catenin Nuclear Immunostaining in Colorectal Cancer Clin. Cancer Res., February 15, 2004; 10(4): 1401 - 1408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S C C Wong, E S F Lo, A K C Chan, K C Lee, and W L Hsiao Nuclear {beta} catenin as a potential prognostic and diagnostic marker in patients with colorectal cancer from Hong Kong Mol. Pathol., December 1, 2003; 56(6): 347 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shiina, M. Igawa, J. Breault, L. Ribeiro-Filho, D. Pookot, S. Urakami, M. Terashima, M. Deguchi, M. Yamanaka, M. Shirai, et al. The Human T-Cell Factor-4 Gene Splicing Isoforms, Wnt Signal Pathway, and Apoptosis in Renal Cell Carcinoma Clin. Cancer Res., June 1, 2003; 9(6): 2121 - 2132. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mareel and A. Leroy Clinical, Cellular, and Molecular Aspects of Cancer Invasion Physiol Rev, April 1, 2003; 83(2): 337 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nabais, J. C. Machado, C. Lopes, R. Seruca, F. Carneiro, and M. Sobrinho-Simoes Patterns of {beta}-Catenin Expression in Gastric Carcinoma: Clinicopathological Relevance and Mutation Analysis International Journal of Surgical Pathology, January 1, 2003; 11(1): 1 - 9. [Abstract] [PDF]
|
|
|
|
 |
|
 T. A. Chan, Z. Wang, L. H. Dang, B. Vogelstein, and K. W. Kinzler Targeted inactivation of CTNNB1 reveals unexpected effects of beta -catenin mutation PNAS, June 11, 2002; 99(12): 8265 - 8270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Harada, H. Miyoshi, N. Murai, H. Oshima, Y. Tamai, M. Oshima, and M. M. Taketo Lack of Tumorigenesis in the Mouse Liver after Adenovirus-mediated Expression of a Dominant Stable Mutant of {beta}-Catenin Cancer Res., April 1, 2002; 62(7): 1971 - 1977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Albuquerque, M Cravo, C Cruz, P Lage, P Chaves, P Fidalgo, A Suspiro, and C Nobre Leitao Genetic characterisation of patients with multiple colonic polyps J. Med. Genet., April 1, 2002; 39(4): 297 - 302. [Full Text] [PDF]
|
|
|
|
|
|
S. Inagawa, M. Itabashi, S. Adachi, T. Kawamoto, M. Hori, J. Shimazaki, F. Yoshimi, and K. Fukao Expression and Prognostic Roles of {beta}-Catenin in Hepatocellular Carcinoma: Correlation with Tumor Progression and Postoperative Survival Clin. Cancer Res., February 1, 2002; 8(2): 450 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Carothers, K. A. Melstrom Jr., J. D. Mueller, M. J. Weyant, and M. M. Bertagnolli Progressive Changes in Adherens Junction Structure during Intestinal Adenoma Formation in Apc Mutant Mice J. Biol. Chem., October 12, 2001; 276(42): 39094 - 39102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Liu, J. Chen, S. Salahshor, S. Kuismanen, E. Holmberg, H. Gronberg, P. Peltomaki, and A. Lindblom Screening families with endometrial and colorectal cancers for germline mutations J. Med. Genet., September 1, 2001; 38(9): e29 - 29. [Full Text] [PDF]
|
|
|
|
|
|
W. Szeto, W. Jiang, D. A. Tice, B. Rubinfeld, P. G. Hollingshead, S. E. Fong, D. L. Dugger, T. Pham, D. G. Yansura, T. A. Wong, et al. Overexpression of the Retinoic Acid-responsive Gene Stra6 in Human Cancers and Its Synergistic Induction by Wnt-1 and Retinoic Acid Cancer Res., May 1, 2001; 61(10): 4197 - 4205. [Abstract] [Full Text]
|
|
|
|
|
|
J. H. Sellin, S. Umar, J. Xiao, and A. P. Morris Increased {beta}-Catenin Expression and Nuclear Translocation Accompany Cellular Hyperproliferation in Vivo Cancer Res., April 1, 2001; 61(7): 2899 - 2906. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. C. Abraham, B. Nobukawa, F. M. Giardiello, S. R. Hamilton, and T.-T. Wu Sporadic Fundic Gland Polyps : Common Gastric Polyps Arising Through Activating Mutations in the {beta}-Catenin Gene Am. J. Pathol., March 1, 2001; 158(3): 1005 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Abraham, E. A. Montgomery, F. M. Giardiello, and T.-T. Wu Frequent {beta}-Catenin Mutations in Juvenile Nasopharyngeal Angiofibromas Am. J. Pathol., March 1, 2001; 158(3): 1073 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fujita, Y. Furukawa, Y. Nagasawa, M. Ogawa, and Y. Nakamura Down-Regulation of Monocyte Chemotactic Protein-3 by Activated {beta}-Catenin Cancer Res., December 1, 2000; 60(23): 6683 - 6687. [Abstract] [Full Text]
|
|
|
|
|
|
S. Maiti, R. Alam, C. I. Amos, and V. Huff Frequent Association of {beta}-Catenin and WT1 Mutations in Wilms Tumors Cancer Res., November 1, 2000; 60(22): 6288 - 6292. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Polakis Wnt signaling and cancer Genes & Dev., August 1, 2000; 14(15): 1837 - 1851. [Full Text]
|
|
|
|
|
|
F. T. Kolligs, B. Kolligs, K. M. Hajra, G. Hu, M. Tani, K. R. Cho, and E. R. Fearon gamma -Catenin is regulated by the APC tumor suppressor and its oncogenic activity is distinct from that of beta -catenin Genes & Dev., June 1, 2000; 14(11): 1319 - 1331. [Abstract] [Full Text]
|
|
|
|
|
|
H. S. Cooper, S. Murthy, K. Kido, H. Yoshitake, and A. Flanigan Dysplasia and cancer in the dextran sulfate sodium mouse colitis model. Relevance to colitis-associated neoplasia in the human: a study of histopathology, B-catenin and p53 expression and the role of inflammation Carcinogenesis, April 1, 2000; 21(4): 757 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bilim, T. Kawasaki, A. Katagiri, S.-j. Wakatsuki, K. Takahashi, and Y. Tomita Altered Expression of {beta}-Catenin in Renal Cell Cancer and Transitional Cell Cancer with the Absence of {beta}-catenin Gene Mutations Clin. Cancer Res., February 1, 2000; 6(2): 460 - 466. [Abstract] [Full Text]
|
|
|
|
|
|
H. Huang, H. Fujii, A. Sankila, B. M. Mahler-Araujo, M. Matsuda, G. Cathomas, and H. Ohgaki {beta}-Catenin Mutations Are Frequent in Human Hepatocellular Carcinomas Associated with Hepatitis C Virus Infection Am. J. Pathol., December 1, 1999; 155(6): 1795 - 1801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyaki, T. Iijima, J. Kimura, M. Yasuno, T. Mori, Y. Hayashi, M. Koike, N. Shitara, T. Iwama, and T. Kuroki Frequent Mutation of {beta}-Catenin and APC Genes in Primary Colorectal Tumors from Patients with Hereditary Nonpolyposis Colorectal Cancer Cancer Res., September 1, 1999; 59(18): 4506 - 4509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. V. Nhieu, C. A. Renard, Y. Wei, D. Cherqui, E. S. Zafrani, and M. A. Buendia Nuclear Accumulation of Mutated {beta}-Catenin in Hepatocellular Carcinoma Is Associated with Increased Cell Proliferation Am. J. Pathol., September 1, 1999; 155(3): 703 - 710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Munné, M. Fabre, M. L. Mariñoso, M. Gallén, F. X. Real, and for the Colon Cancer Team at IMAS Nuclear {beta}-catenin in Colorectal Tumors: To Freeze or Not To Freeze? J. Histochem. Cytochem., August 1, 1999; 47(8): 1089 - 1094. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Gradl, M. Kuhl, and D. Wedlich The Wnt/Wg Signal Transducer beta -Catenin Controls Fibronectin Expression Mol. Cell. Biol., August 1, 1999; 19(8): 5576 - 5587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nagasawa, Y. Miyoshi, K. Iwao, Y. Shinomura, Y. Matsuzawa, and Y. Nakamura Transformation and Morphological Changes of Murine L Cells by Transfection with a Mutated Form of {beta}-Catenin Cancer Res., August 1, 1999; 59(15): 3539 - 3542. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Romagnolo, D. Berrebi, S. Saadi-Keddoucci, A. Porteu, A.-l. Pichard, M. Peuchmaur, A. Vandewalle, A. Kahn, and C. Perret Intestinal Dysplasia and Adenoma in Transgenic Mice after Overexpression of an Activated {beta}-Catenin Cancer Res., August 1, 1999; 59(16): 3875 - 3879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gamallo, J. Palacios, G. Moreno, J. Calvo de Mora, A. Suarez, and A. Armas {beta}-Catenin Expression Pattern in Stage I and II Ovarian Carcinomas : Relationship with {beta}-Catenin Gene Mutations,Clinicopathological Features, and Clinical Outcome Am. J. Pathol., August 1, 1999; 155(2): 527 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mirabelli-Primdahl, R. Gryfe, H. Kim, A. Millar, C. Luceri, D. Dale, E. Holowaty, B. Bapat, S. Gallinger, and M. Redston {beta}-Catenin Mutations Are Specific for Colorectal Carcinomas with Microsatellite Instability but Occur in Endometrial Carcinomas Irrespective of Mutator Pathway Cancer Res., July 1, 1999; 59(14): 3346 - 3351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. DOBBIE and H. MÜLLER Germline mutations in the beta -catenin gene are not associated with the FAP phenotype without an APC mutation J. Med. Genet., July 1, 1999; 36(7): 573 - 574. [Full Text]
|
|
|
|
 |
|
 K. Caca, F. T. Kolligs, X. Ji, M. Hayes, J.-m. Qian, A. Yahanda, D. L. Rimm, J. Costa, and E. R. Fearon {beta}- and {{gamma}}-Catenin Mutations, but not E-Cadherin Inactivation, Underlie T-Cell Factor/Lymphoid Enhancer Factor Transcriptional Deregulation in Gastric and Pancreatic Cancer Cell Growth Differ., June 1, 1999; 10(6): 369 - 376. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. Pötter, C. Bergwitz, and G. Brabant The Cadherin-Catenin System: Implications for Growth and Differentiation of Endocrine Tissues Endocr. Rev., April 1, 1999; 20(2): 207 - 239. [Abstract] [Full Text]
|
|
|
|
|
|
W. S. Samowitz, M. D. Powers, L. N. Spirio, F. Nollet, F. van Roy, and M. L. Slattery {beta}-Catenin Mutations Are More Frequent in Small Colorectal Adenomas Than in Larger Adenomas and Invasive Carcinomas Cancer Res., April 1, 1999; 59(7): 1442 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Garcia-Rostan, G. Tallini, A. Herrero, T. G. D'Aquila, M. L. Carcangiu, and D. L. Rimm Frequent Mutation and Nuclear Localization of {beta}-Catenin in Anaplastic Thyroid Carcinoma Cancer Res., April 1, 1999; 59(8): 1811 - 1815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Yokoya, N. Imamoto, T. Tachibana, and Y. Yoneda beta -Catenin Can Be Transported into the Nucleus in a Ran-unassisted Manner Mol. Biol. Cell, April 1, 1999; 10(4): 1119 - 1131. [Abstract] [Full Text]
|
|
|
|
|
|
D. L. Rimm, K. Caca, G. Hu, F. B. Harrison, and E. R. Fearon Frequent Nuclear/Cytoplasmic Localization of ß-Catenin without Exon 3 Mutations in Malignant Melanoma Am. J. Pathol., February 1, 1999; 154(2): 325 - 329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Koch, D. Denkhaus, S. Albrecht, I. Leuschner, D. von Schweinitz, and T. Pietsch Childhood Hepatoblastomas Frequently Carry a Mutated Degradation Targeting Box of the {beta}-Catenin Gene Cancer Res., January 1, 1999; 59(2): 269 - 273. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
