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Increased ß-Actin Expression in an Invasive Moloney Sarcoma Virus-transformed MDCK Cell Variant Concentrates to the Tips of Multiple Pseudopodia¹

Département de Pathologie et Biologie Cellulaire, Université de Montréal, Montréal, Québec, Canada H3T 1J4

An invasive variant of Moloney sarcoma virus-transformed MDCK cells (MSV-MDCK-INV), which was isolated by the repeated selection of cells that successfully traversed a Matrigel-coated filter, exhibits increased motile ability and presents an elongated cell shape and numerous pseudopodia. Although stress fibers are present in both MDCK and MSV-MDCK cells, MSV-MDCK-INV cells contain no stress fibers and exhibit a dense concentration of actin at the tips of pseudopodia. Relative to both MDCK and MSV-MDCK cells, the MSV-MDCK-INV cells exhibit increased expression of ß-actin and redistribution of ß-actin to the tips of pseudopodia. These actin concentrations are enriched in both F- and G-actin and, thus, represent dynamic regions of actin cytoskeleton remodeling. The acquisition of invasive properties by epithelial transformants is, therefore, associated with the increased expression of ß-actin and its concentration in actin-rich domains, which may drive pseudopodial extension and facilitate tumor cell invasion.

¹ These studies were supported by grants from the National Cancer Institute of Canada (to I. R. N.) with money raised by the Canadian Cancer Society and from the Medical Research Council of Canada (to N. L. and I. R. N.) and by salary support from the Fonds de la Recherche en Santé du Québec (to N. L. and I. R. N.).

² To whom requests for reprints should be addressed, at Département de Pathologie et Biologie Cellulaire, N-812, Université de Montréal, 2900 Edouard Montpetit, Montréal, Québec, Canada H3T 1J4. Phone: (514) 343-6291; E-mail: nabii@ere.umontreal.ca.

Received 12/16/97. Accepted 3/ 3/98.

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