This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Grassilli, E. Articles by Calabretta, B. Search for Related Content PubMed PubMed Citation Articles by Grassilli, E. Articles by Calabretta, B.

Resistance to Apoptosis in CTLL-2 Cells Overexpressing B-Myb Is Associated with B-Myb-dependent bcl-2 Induction¹

Department of Microbiology and Immunology and Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 [E. G., P. S., D. P., B. C.]; and Department of Biomedical Sciences, Section of General Pathology, University of Modena, 41100, Modena, Italy [E. G., P. S., C. F.]

Transcriptional regulators of the Myb family play important roles in cell proliferation, differentiation, and survival. To investigate the role of Myb proteins in the regulation of apoptosis, we studied the apoptotic response of interleukin 2-dependent CTLL-2 cells stably transfected with B-Myb. B-Myb-overexpressing cells showed a diminished cytokine dependence and were resistant to apoptosis induced by doxorubicin, ceramide, and dexamethasone. Overexpression of B-Myb was associated with enhanced expression of bcl-2, which was dependent, at least in part, on increased transcription. In transient transfection assays in T-lymphoblastic cells, B-Myb was able to stimulate the promoter activity of the bcl-2 5' flanking region linked to the chloramphenicol acetyltransferase reporter gene. A segment of the bcl-2 promoter (nucleotides +34 to +58 relative to the transcription initiation site) contained a putative Myb-binding site and was shown to specifically interact with B-Myb and to confer B-Myb responsiveness to a bcl-2/chloramphenicol acetyltransferase reporter construct. These results indicate that B-Myb promotes T cells survival by enhancing the expression of bcl-2 and identify bcl-2 as a B-Myb target gene regulated in a DNA binding-dependent manner.

This article has been cited by other articles:

				T. Rampias, C. Sasaki, P. Weinberger, and A. Psyrri E6 and E7 Gene Silencing and Transformed Phenotype of Human Papillomavirus 16-Positive Oropharyngeal Cancer Cells J Natl Cancer Inst, March 18, 2009; 101(6): 412 - 423. [Abstract] [Full Text] [PDF]

				G. Santilli, R. Schwab, R. Watson, C. Ebert, B. J. Aronow, and A. Sala Temperature-dependent Modification and Activation of B-MYB: IMPLICATIONS FOR CELL SURVIVAL J. Biol. Chem., April 22, 2005; 280(16): 15628 - 15634. [Abstract] [Full Text] [PDF]

				D. X. Liu, S. C. Biswas, and L. A. Greene B-Myb and C-Myb Play Required Roles in Neuronal Apoptosis Evoked by Nerve Growth Factor Deprivation and DNA Damage J. Neurosci., October 6, 2004; 24(40): 8720 - 8725. [Abstract] [Full Text] [PDF]

				Q. Wu, P. Kirschmeier, T. Hockenberry, T.-Y. Yang, D. L. Brassard, L. Wang, T. McClanahan, S. Black, G. Rizzi, M. L. Musco, et al. Transcriptional Regulation during p21WAF1/CIP1-induced Apoptosis in Human Ovarian Cancer Cells J. Biol. Chem., September 20, 2002; 277(39): 36329 - 36337. [Abstract] [Full Text] [PDF]

				A. Lauder, A. Castellanos, and K. Weston c-myb Transcription Is Activated by Protein Kinase B (PKB) following Interleukin 2 Stimulation of T Cells and Is Required for PKB-Mediated Protection from Apoptosis Mol. Cell. Biol., September 1, 2001; 21(17): 5797 - 5805. [Abstract] [Full Text] [PDF]

				M. A. Powzaniuk, R. Trotta, M. J. Loza, A. Harth, R. V. Iozzo, L. C. Eisenlohr, B. Perussia, and B. Calabretta B-Myb Overexpression Results in Activation and Increased Fas/Fas Ligand-Mediated Cytotoxicity of T and NK Cells J. Immunol., July 1, 2001; 167(1): 242 - 249. [Abstract] [Full Text] [PDF]

				P. G. Lutz, A. Houzel-Charavel, C. Moog-Lutz, and Y. E. Cayre Myeloblastin is an Myb target gene: mechanisms of regulation in myeloid leukemia cells growth-arrested by retinoic acid Blood, April 15, 2001; 97(8): 2449 - 2456. [Abstract] [Full Text] [PDF]

				C. Muller-Tidow, W. Wang, G. E. Idos, S. Diederichs, R. Yang, C. Readhead, W. E. Berdel, H. Serve, M. Saville, R. Watson, et al. Cyclin A1 directly interacts with B-myb and cyclin A1/cdk2 phosphorylate B-myb at functionally important serine and threonine residues: tissue-specific regulation of B-myb function Blood, April 1, 2001; 97(7): 2091 - 2097. [Abstract] [Full Text] [PDF]

				G. Gao and Q. P. Dou G1 Phase-Dependent Expression of Bcl-2 mRNA and Protein Correlates with Chemoresistance of Human Cancer Cells Mol. Pharmacol., November 1, 2000; 58(5): 1001 - 1010. [Abstract] [Full Text]

				C. A. Heckman, J. W. Mehew, G.-G. Ying, M. Introna, J. Golay, and L. M. Boxer A-Myb Up-regulates Bcl-2 through a Cdx Binding Site in t(14;18) Lymphoma Cells J. Biol. Chem., February 25, 2000; 275(9): 6499 - 6508. [Abstract] [Full Text] [PDF]

				M. Cervellera, G. Raschella, G. Santilli, B. Tanno, A. Ventura, C. Mancini, C. Sevignani, B. Calabretta, and A. Sala Direct Transactivation of the Anti-apoptotic Gene Apolipoprotein J (Clusterin) by B-MYB J. Biol. Chem., July 7, 2000; 275(28): 21055 - 21060. [Abstract] [Full Text] [PDF]