Cancer Research Translational Cancer Medicine 2008: Cancer Clinical Trials and Personalized Medicine Susan G. Komen for the Cure-AACR Outstanding Investigator Award for Breast Cancer Research
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Cell Growth & Differentiation
[Cancer Research 59, 2522-2526, June 1, 1999]
© 1999 American Association for Cancer Research
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakamura, J.
Right arrow Articles by Swenberg, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakamura, J.
Right arrow Articles by Swenberg, J. A.
[Cancer Research 59, 2522-2526, June 1, 1999]
© 1999 American Association for Cancer Research

Advances in Brief

Endogenous Apurinic/Apyrimidinic Sites in Genomic DNA of Mammalian Tissues1

Jun Nakamura and James A. Swenberg2

Department of Environmental Sciences and Engineering, The University of North Carolina, Chapel Hill, North Carolina 27599

Apurinic/apyrimidinic (AP) sites are one of the most frequent lesions in DNA. Using a highly sensitive slot blot assay, we determined the number and condition of endogenous AP sites in normal tissues of rats and human liver. The number of AP sites (50,000–200,000 per mammalian cell) was greatest in brain, followed by colon and heart, and then liver, lung, and kidney. The majority of endogenous AP sites were cleaved 5' to the AP site. These data suggest that removal of the deoxyribosyl phosphate moiety is the rate-limiting step in base excision and AP site repair in vivo.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. Xiao, Y. Su, R. C. M. Simmen, and F. A. Simmen
Dietary soy protein inhibits DNA damage and cell survival of colon epithelial cells through attenuated expression of fatty acid synthase
Am J Physiol Gastrointest Liver Physiol, April 1, 2008; 294(4): G868 - G876.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
C. G. Woods, A. M. Burns, B. U. Bradford, P. K. Ross, O. Kosyk, J. A. Swenberg, M. L. Cunningham, and I. Rusyn
WY-14,643 Induced Cell Proliferation and Oxidative Stress in Mouse Liver are Independent of NADPH Oxidase
Toxicol. Sci., August 1, 2007; 98(2): 366 - 374.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Fiala and Z. Suo
Sloppy Bypass of an Abasic Lesion Catalyzed by a Y-family DNA Polymerase
J. Biol. Chem., March 16, 2007; 282(11): 8199 - 8206.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Wang, C. C. Shupenko, L. F. Melo, and P. R. Strauss
DNA Repair Protein Involved in Heart and Blood Development
Mol. Cell. Biol., December 1, 2006; 26(23): 9083 - 9093.
[Abstract] [Full Text] [PDF]

Home page
 Radiat Prot DosimetryHome page
L. B. Smilenov, E. J. Hall, W. M. Bonner, and O. A. Sedelnikova
A microbeam study of DNA double-strand breaks in bystander primary human fibroblasts
Radiat Prot Dosimetry, December 1, 2006; 122(1-4): 256 - 259.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
P. D. Chastain II, J. Nakamura, J. Swenberg, and D. Kaufman
Nonrandom AP site distribution in highly proliferative cells
FASEB J, December 1, 2006; 20(14): 2612 - 2614.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
C. L. Powell, O. Kosyk, P. K. Ross, R. Schoonhoven, G. Boysen, J. A. Swenberg, A. N. Heinloth, G. A. Boorman, M. L. Cunningham, R. S. Paules, et al.
Phenotypic Anchoring of Acetaminophen-Induced Oxidative Stress with Gene Expression Profiles in Rat Liver
Toxicol. Sci., September 1, 2006; 93(1): 213 - 222.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Jin, W. S. May, F. Gao, T. Flagg, and X. Deng
Bcl2 Suppresses DNA Repair by Enhancing c-Myc Transcriptional Activity
J. Biol. Chem., May 19, 2006; 281(20): 14446 - 14456.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Takeuchi, T. Ruike, R.-i. Nakamura, K. Shimanouchi, Y. Kanai, Y. Abe, A. Ihara, and K. Sakaguchi
Drosophila DNA Polymerase {zeta} Interacts with Recombination Repair Protein 1, the Drosophila Homologue of Human Abasic Endonuclease 1
J. Biol. Chem., April 28, 2006; 281(17): 11577 - 11585.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. Seiple, P. Jaruga, M. Dizdaroglu, and J. T. Stivers
Linking uracil base excision repair and 5-fluorouracil toxicity in yeast
Nucleic Acids Res., January 10, 2006; 34(1): 140 - 151.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-S. Sung, M. S. DeMott, and B. Demple
Long-patch Base Excision DNA Repair of 2-Deoxyribonolactone Prevents the Formation of DNA-Protein Cross-links with DNA Polymerase {beta}
J. Biol. Chem., November 25, 2005; 280(47): 39095 - 39103.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y.-C. Jeong, J. Nakamura, P. B. Upton, and J. A. Swenberg
Pyrimido[1,2-a]-purin-10(3H)-one, M1G, is less prone to artifact than base oxidation
Nucleic Acids Res., November 10, 2005; 33(19): 6426 - 6434.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
C. M. Ulrich, K. Curtin, J. D. Potter, J. Bigler, B. Caan, and M. L. Slattery
Polymorphisms in the Reduced Folate Carrier, Thymidylate Synthase, or Methionine Synthase and Risk of Colon Cancer
Cancer Epidemiol. Biomarkers Prev., November 1, 2005; 14(11): 2509 - 2516.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
C. M. Ulrich
Nutrigenetics in Cancer Research--Folate Metabolism and Colorectal Cancer
J. Nutr., November 1, 2005; 135(11): 2698 - 2702.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Madhusudan, F. Smart, P. Shrimpton, J. L. Parsons, L. Gardiner, S. Houlbrook, D. C. Talbot, T. Hammonds, P. A. Freemont, M. J. E. Sternberg, et al.
Isolation of a small molecule inhibitor of DNA base excision repair
Nucleic Acids Res., August 19, 2005; 33(15): 4711 - 4724.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. F. Harrison, S. B. Hollensworth, D. R. Spitz, W. C. Copeland, G. L. Wilson, and S. P. LeDoux
Oxidative stress-induced apoptosis in neurons correlates with mitochondrial DNA base excision repair pathway imbalance
Nucleic Acids Res., August 17, 2005; 33(14): 4660 - 4671.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Sossou, C. Flohr-Beckhaus, I. Schulz, F. Daboussi, B. Epe, and J. P. Radicella
APE1 overexpression in XRCC1-deficient cells complements the defective repair of oxidative single strand breaks but increases genomic instability
Nucleic Acids Res., January 12, 2005; 33(1): 298 - 306.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. G. Georgakilas, P. V. Bennett, D. M. Wilson III, and B. M. Sutherland
Processing of bistranded abasic DNA clusters in {gamma}-irradiated human hematopoietic cells
Nucleic Acids Res., October 19, 2004; 32(18): 5609 - 5620.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Huamani, C. A. McMahan, D. C. Herbert, R. Reddick, J. R. McCarrey, M. I. MacInnes, D. J. Chen, and C. A. Walter
Spontaneous Mutagenesis Is Enhanced in Apex Heterozygous Mice
Mol. Cell. Biol., September 15, 2004; 24(18): 8145 - 8153.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J. Zielinska-Park, J. Nakamura, J. A. Swenberg, and M. D. Aitken
Aldehydic DNA lesions in calf thymus DNA and HeLa S3 cells produced by bacterial quinone metabolites of fluoranthene and pyrene
Carcinogenesis, September 1, 2004; 25(9): 1727 - 1733.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wong and B. Demple
Modulation of the 5'-Deoxyribose-5-phosphate Lyase and DNA Synthesis Activities of Mammalian DNA Polymerase {beta} by Apurinic/Apyrimidinic Endonuclease 1
J. Biol. Chem., June 11, 2004; 279(24): 25268 - 25275.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
R. De Bont and N. van Larebeke
Endogenous DNA damage in humans: a review of quantitative data
Mutagenesis, May 1, 2004; 19(3): 169 - 185.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. Raffoul, D. C. Cabelof, J. Nakamura, L. B. Meira, E. C. Friedberg, and A. R. Heydari
Apurinic/Apyrimidinic Endonuclease (APE/REF-1) Haploinsufficient Mice Display Tissue-specific Differences in DNA Polymerase {beta}-Dependent Base Excision Repair
J. Biol. Chem., April 30, 2004; 279(18): 18425 - 18433.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Ribar, T. Izumi, and S. Mitra
The major role of human AP-endonuclease homolog Apn2 in repair of abasic sites in Schizosaccharomyces pombe
Nucleic Acids Res., January 2, 2004; 32(1): 115 - 126.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
I. Zurer, L. J. Hofseth, Y. Cohen, M. Xu-Welliver, S. P. Hussain, C. C. Harris, and V. Rotter
The role of p53 in base excision repair following genotoxic stress
Carcinogenesis, January 1, 2004; 25(1): 11 - 19.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Kokoska, S. D. McCulloch, and T. A. Kunkel
The Efficiency and Specificity of Apurinic/Apyrimidinic Site Bypass by Human DNA Polymerase {eta} and Sulfolobus solfataricus Dpo4
J. Biol. Chem., December 12, 2003; 278(50): 50537 - 50545.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Guillet and S. Boiteux
Origin of Endogenous DNA Abasic Sites in Saccharomyces cerevisiae
Mol. Cell. Biol., November 15, 2003; 23(22): 8386 - 8394.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
A. Barbin, H. Ohgaki, J. Nakamura, M. Kurrer, P. Kleihues, and J. A. Swenberg
Endogenous Deoxyribonucleic Acid (DNA) Damage in Human Tissues: A Comparison of Ethenobases with Aldehydic DNA Lesions
Cancer Epidemiol. Biomarkers Prev., November 1, 2003; 12(11): 1241 - 1247.
[Abstract] [Full Text]

Home page
 Nucleic Acids ResHome page
K. L. Meadows, B. Song, and P. W. Doetsch
Characterization of AP lyase activities of Saccharomyces cerevisiae Ntg1p and Ntg2p: implications for biological function
Nucleic Acids Res., October 1, 2003; 31(19): 5560 - 5567.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wong, M. S. DeMott, and B. Demple
Modulation of the 3'->5'-Exonuclease Activity of Human Apurinic Endonuclease (Ape1) by Its 5'-incised Abasic DNA Product
J. Biol. Chem., September 19, 2003; 278(38): 36242 - 36249.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
L. Calderon-Garciduenas, R. R. Maronpot, R. Torres-Jardon, C. Henriquez-Roldan, R. Schoonhoven, H. Acuna-Ayala, A. Villarreal-Calderon, J. Nakamura, R. Fernando, W. Reed, et al.
DNA Damage in Nasal and Brain Tissues of Canines Exposed to Air Pollutants Is Associated with Evidence of Chronic Brain Inflammation and Neurodegeneration
Toxicol Pathol, August 1, 2003; 31(5): 524 - 538.
[Abstract] [PDF]

Home page
 CarcinogenesisHome page
P.-H. Lin, J. Nakamura, S. Yamaguchi, S. Asakura, and J. A. Swenberg
Aldehydic DNA lesions induced by catechol estrogens in calf thymus DNA
Carcinogenesis, June 1, 2003; 24(6): 1133 - 1141.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Kamiya
Mutagenic potentials of damaged nucleic acids produced by reactive oxygen/nitrogen species: approaches using synthetic oligonucleotides and nucleotides: SURVEY AND SUMMARY
Nucleic Acids Res., January 15, 2003; 31(2): 517 - 531.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. R. Silber, M. S. Bobola, A. Blank, K. D. Schoeler, P. D. Haroldson, M. B. Huynh, and D. D. Kolstoe
The Apurinic/Apyrimidinic Endonuclease Activity of Ape1/Ref-1 Contributes to Human Glioma Cell Resistance to Alkylating Agents and Is Elevated by Oxidative Stress
Clin. Cancer Res., September 1, 2002; 8(9): 3008 - 3018.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. G. Georgakilas, P. V. Bennett, and B. M. Sutherland
High efficiency detection of bi-stranded abasic clusters in {gamma}-irradiated DNA by putrescine
Nucleic Acids Res., July 1, 2002; 30(13): 2800 - 2808.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. M. Ulrich, J. Bigler, R. Bostick, L. Fosdick, and J. D. Potter
Thymidylate Synthase Promoter Polymorphism, Interaction with Folate Intake, and Risk of Colorectal Adenomas
Cancer Res., June 1, 2002; 62(12): 3361 - 3364.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. W. Sobol, D. E. Watson, J. Nakamura, F. M. Yakes, E. Hou, J. K. Horton, J. Ladapo, B. Van Houten, J. A. Swenberg, K. R. Tindall, et al.
Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress
PNAS, May 14, 2002; 99(10): 6860 - 6865.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Z. Fan, P. J. Beresford, D. Zhang, and J. Lieberman
HMG2 Interacts with the Nucleosome Assembly Protein SET and Is a Target of the Cytotoxic T-Lymphocyte Protease Granzyme A
Mol. Cell. Biol., April 15, 2002; 22(8): 2810 - 2820.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. M. Flaherty, M. M. Monick, and G. W. Hunninghake
AP Endonucleases and the Many Functions of Ref-1
Am. J. Respir. Cell Mol. Biol., December 1, 2001; 25(6): 664 - 667.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Wilstermann and N. Osheroff
Base Excision Repair Intermediates as Topoisomerase II Poisons
J. Biol. Chem., November 30, 2001; 276(49): 46290 - 46296.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Fung, R. A. O. Bennett, and B. Demple
Key Role of a Downstream Specificity Protein 1 Site in Cell Cycle-regulated Transcription of the AP Endonuclease Gene APE1/APEX in NIH3T3 Cells
J. Biol. Chem., November 2, 2001; 276(45): 42011 - 42017.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
P.-H. Lin, J. Nakamura, S. Yamaguchi, P. B. Upton, D. K. La, and J. A. Swenberg
Oxidative damage and direct adducts in calf thymus DNA induced by the pentachlorophenol metabolites, tetrachlorohydroquinone and tetrachloro-1,4-benzoquinone
Carcinogenesis, April 1, 2001; 22(4): 627 - 634.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
P.-H. Lin, J. Nakamura, S. Yamaguchi, D. K. La, P. B. Upton, and J. A. Swenberg
Induction of direct adducts, apurinic/apyrimidinic sites and oxidized bases in nuclear DNA of human HeLa S3 tumor cells by tetrachlorohydroquinone
Carcinogenesis, April 1, 2001; 22(4): 635 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. R. Kelley, L. Cheng, R. Foster, R. Tritt, J. Jiang, J. Broshears, and M. Koch
Elevated and Altered Expression of the Multifunctional DNA Base Excision Repair and Redox Enzyme Ape1/ref-1 in Prostate Cancer
Clin. Cancer Res., April 1, 2001; 7(4): 824 - 830.
[Abstract] [Full Text]

Home page
 CarcinogenesisHome page
M. N. Rios-Blanco, T. H. Faller, J. Nakamura, W. Kessler, P. E. Kreuzer, A. Ranasinghe, J. G. Filser, and J. A. Swenberg
Quantitation of DNA and hemoglobin adducts and apurinic/apyrimidinic sites in tissues of F344 rats exposed to propylene oxide by inhalation
Carcinogenesis, November 1, 2000; 21(11): 2011 - 2018.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Sabourin and N. Osheroff
Sensitivity of human type II topoisomerases to DNA damage: stimulation of enzyme-mediated DNA cleavage by abasic, oxidized and alkylated lesions
Nucleic Acids Res., May 1, 2000; 28(9): 1947 - 1954.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
L. J. Marnett
Oxyradicals and DNA damage
Carcinogenesis, March 1, 2000; 21(3): 361 - 370.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Nakamura, D. K. La, and J. A. Swenberg
5'-Nicked Apurinic/Apyrimidinic Sites Are Resistant to beta -Elimination by beta -Polymerase and Are Persistent in Human Cultured Cells after Oxidative Stress
J. Biol. Chem., February 25, 2000; 275(8): 5323 - 5328.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. K. Horton, R. Prasad, E. Hou, and S. H. Wilson
Protection against Methylation-induced Cytotoxicity by DNA Polymerase beta -Dependent Long Patch Base Excision Repair
J. Biol. Chem., January 21, 2000; 275(3): 2211 - 2218.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Atamna, I. Cheung, and B. N. Ames
A method for detecting abasic sites in living cells: Age-dependent changes in base excision repair
PNAS, January 18, 2000; 97(2): 686 - 691.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. A. Ranalli, M. S. DeMott, and R. A. Bambara
Mechanism Underlying Replication Protein A Stimulation of DNA Ligase I
J. Biol. Chem., January 11, 2002; 277(3): 1719 - 1727.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Cell Growth & Differentiation
Copyright © 1999 by the American Association for Cancer Research.