Cancer Research Grants Advances in Breast Cancer Research
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
[Cancer Research 59, 2787-2790, June 1, 1999]
© 1999 American Association for Cancer Research
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Degeorges, A.
Right arrow Articles by Sikes, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Degeorges, A.
Right arrow Articles by Sikes, R. A.
[Cancer Research 59, 2787-2790, June 15, 1999]
© 1999 American Association for Cancer Research

Advances in Brief

Human Prostate Cancer Expresses the Low Affinity Insulin-like Growth Factor Binding Protein IGFBP-rP11

Armelle Degeorges, Fuan Wang, Henry F. Frierson, Jr., Arun Seth, Leland W. K. Chung and Robert A. Sikes2

Department of Urology, Molecular Urology and Therapeutics Program [A. D., F. W., L. W. K. C., R. A. S.] and Department of Pathology [H. F. F.], University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, and Department of Laboratory Medicine and Pathobiology, MRC Group in Periodontal Physiology and Women’s College Hospital, University of Toronto, Toronto ON M5S 1B2, Canada [A. S.]

Many of the alterations in the insulin-like growth factor (IGF) axis in prostatic disease have been associated with changes in the insulin-like growth factor binding proteins (IGFBPs), a multigene family of proteins that are thought to mediate the action of IGFs on target tissues. IGFBP-related protein 1 (rP1), also known as IGFBP-7 or mac25, is a recently described member of the IGFBP family, the biological function of which has yet to be completely ascertained. In this study, we analyzed the localization of IGFBP-rP1 in prostate cancer and benign prostate tissues using immunohistochemistry and a polyclonal antibody, T1A12, that is specific for IGFBP-rP1. The most intense staining was observed in nerves, whereas smooth muscle cells in the prostate stained weakly. Lymphocytes were always negative. When normal prostatic secretory epithelium was present, staining was usually absent. The lining secretory epithelium stained positively in 0 of 12 (0%) cases of benign prostatic hyperplasia, 57 of 63 (90.5%) primary adenocarcinomas, and 7 of 7 (100%) prostate cancer metastases. Prostatic intraepithelial neoplasia showed a similar pattern of staining to that observed for the invasive tumors. Analysis of Northern blots showed that none of the prostate cancer cell lines (LNCaP, C4, C4-2, C4-2B4, 9069E3, DU145, and PC3) expressed IGFBP-rP1 mRNA. This lack of expression was confirmed by immunohistochemistry of s.c.-generated tumor xenografts of LNCaP and C4-2 and by immunoblot on serum-free-conditioned media from all prostatic cell lines. In contrast to these results, tumor xenografts generated by direct intraosseous injection of LNCaP or C4-2 to bone marrow space resulted in tumors that stained positively for IGFBP-rP1. Our results show that IGFBP-rP1 is expressed in both in situ and invasive prostate neoplasms, but not typically in normal secretory or BPH epithelium; furthermore, the expression of IGFBP-rP1 can be induced in human prostate cancer cell lines in vivo on interaction with an appropriate host environment.




This article has been cited by other articles:


Home page
 J. Clin. Endocrinol. Metab.Home page
A. Lopez-Bermejo, J. Khosravi, C. L. Corless, R. G. Krishna, A. Diamandi, U. Bodani, E. M. Kofoed, D. L. Graham, V. Hwa, and R. G. Rosenfeld
Generation of Anti-Insulin-Like Growth Factor-Binding Protein-Related Protein 1 (IGFBP-rP1/MAC25) Monoclonal Antibodies and Immunoassay: Quantification of IGFBP-rP1 in Human Serum and Distribution in Human Fluids and Tissues
J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3401 - 3408.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pathol.Home page
M Maillard, B Cadot, R Y Ball, K Sethia, D R Edwards, B Perbal, and R Tatoud
Differential expression of the ccn3 (nov) proto-oncogene in human prostate cell lines and tissues
Mol. Pathol., August 1, 2001; 54(4): 275 - 280.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Lopez-Bermejo, C. K. Buckway, G. R. Devi, V. Hwa, S. R. Plymate, Y. Oh, and R. G. Rosenfeld
Characterization of Insulin-Like Growth Factor-Binding Protein-Related Proteins (IGFBP-rPs) 1, 2, and 3 in Human Prostate Epithelial Cells: Potential Roles for IGFBP-rP1 and 2 in Senescence of the Prostatic Epithelium
Endocrinology, November 1, 2000; 141(11): 4072 - 4080.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. Degeorges, F. Wang, H. F. Frierson , Jr., A. Seth, and R. A. Sikes
Distribution of IGFBP-rP1 in Normal Human Tissues
J. Histochem. Cytochem., June 1, 2000; 48(6): 747 - 754.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 1999 by the American Association for Cancer Research.