Cancer Research Versailles No Abst Advances in Breast Cancer Research
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
[Cancer Research 59, 2869-2874, June 1, 1999]
© 1999 American Association for Cancer Research
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burg, M. A.
Right arrow Articles by Stallcup, W. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burg, M. A.
Right arrow Articles by Stallcup, W. B.
[Cancer Research 59, 2869-2874, June 15, 1999]
© 1999 American Association for Cancer Research

Carcinogenesis

NG2 Proteoglycan-binding Peptides Target Tumor Neovasculature1

Michael A. Burg2, Renata Pasqualini, Wadih Arap, Erkki Ruoslahti and William B. Stallcup3

Cancer Research Center, The Burnham Institute, La Jolla, California 92037

NG2 is the rat homologue of the human melanoma proteoglycan, also known as the high molecular weight melanoma-associated antigen. This developmentally regulated membrane-spanning chondroitin sulfate proteoglycan is expressed primarily by glial, muscle, and cartilage progenitor cells. Upon maturation, these cell types down-regulate NG2 expression. In adult animals, the expression of NG2 is restricted to tumor cells and angiogenic tumor vasculature, making this proteoglycan a potential target for directing therapeutic agents to relevant sites of action. To this end, we have identified specific NG2-binding peptides by screening a phage-displayed random peptide library on purified NG2. Several rounds of biopanning on NG2 resulted in the specific enrichment of two phage-displayed decapeptides, TAASGVRSMH and LTLRWVGLMS. The binding of these phages to NG2 was inhibitable both by soluble NG2 and by glutathione S-transferase (GST) fusion proteins containing the cognate peptide sequences. In addition, direct binding between GST-TAASGVRSMH and GST-LTLRWVGLMS fusion proteins and NG2 was demonstrated in solid-phase binding assays. Interestingly, these NG2-binding fusion proteins cross-inhibited each other’s binding to NG2, suggesting that the two sequences bind to the same or overlapping sites on the proteoglycan. Upon injection into tumor-bearing mice, NG2-binding phages specifically homed to tumor vasculature in wild-type mice but did not localize to the tumor vasculature in NG2 knockout mice. The in vivo targeting capability of these sequences suggests that they can be used for tumor targeting.




This article has been cited by other articles:


Home page
 FASEB J.Home page
J. Lahdenranta, R. L. Sidman, R. Pasqualini, and W. Arap
Treatment of hypoxia-induced retinopathy with targeted proapoptotic peptidomimetic in a mouse model of disease
FASEB J, October 1, 2007; 21(12): 3272 - 3278.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. L. Arbiser, T. Kau, M. Konar, K. Narra, R. Ramchandran, S. A. Summers, C. J. Vlahos, K. Ye, B. N. Perry, W. Matter, et al.
Solenopsin, the alkaloidal component of the fire ant (Solenopsis invicta), is a naturally occurring inhibitor of phosphatidylinositol-3-kinase signaling and angiogenesis
Blood, January 15, 2007; 109(2): 560 - 565.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
Y. Oh, I. Mohiuddin, Y. Sun, J. B. Putnam Jr, W. K. Hong, W. Arap, and R. Pasqualini
Phenotypic Diversity of the Lung Vasculature in Experimental Models of Metastases
Chest, December 1, 2005; 128(6_suppl): 596S - 600S.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
Y. Oh, I. Mohiuddin, Y. Sun, J. B. Putnam Jr, W. K. Hong, W. Arap, and R. Pasqualini
Phenotypic Diversity of the Lung Vasculature in Experimental Models of Metastases
Chest, December 1, 2005; 128(6_suppl): 596S - 600S.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. Kessler, R. Bieker, T. Padro, C. Schwoppe, T. Persigehl, C. Bremer, M. Kreuter, W. E. Berdel, and R. M. Mesters
Inhibition of Tumor Growth by RGD Peptide-Directed Delivery of Truncated Tissue Factor to the Tumor Vasculature
Clin. Cancer Res., September 1, 2005; 11(17): 6317 - 6324.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
S. Zitzmann, W. Mier, A. Schad, R. Kinscherf, V. Askoxylakis, S. Kramer, A. Altmann, M. Eisenhut, and U. Haberkorn
A New Prostate Carcinoma Binding Peptide (DUP-1) for Tumor Imaging and Therapy
Clin. Cancer Res., January 1, 2005; 11(1): 139 - 146.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
S. Hughes and T. Chan-Ling
Characterization of Smooth Muscle Cell and Pericyte Differentiation in the Rat Retina In Vivo
Invest. Ophthalmol. Vis. Sci., August 1, 2004; 45(8): 2795 - 2806.
[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
Y Li, M C Madigan, K Lai, R M Conway, F A Billson, R Crouch, and B J Allen
Human uveal melanoma expresses NG2 immunoreactivity
Br. J. Ophthalmol., May 1, 2003; 87(5): 629 - 632.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
T. Nakamura, K. Sato, and H. Hamada
Reduction of Natural Adenovirus Tropism to the Liver by both Ablation of Fiber-Coxsackievirus and Adenovirus Receptor Interaction and Use of Replaceable Short Fiber
J. Virol., February 15, 2003; 77(4): 2512 - 2521.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Sevigny, C. Sundberg, N. Braun, O. Guckelberger, E. Csizmadia, I. Qawi, M. Imai, H. Zimmermann, and S. C. Robson
Differential catalytic properties and vascular topography of murine nucleoside triphosphate diphosphohydrolase 1 (NTPDase1) and NTPDase2 have implications for thromboregulation
Blood, April 15, 2002; 99(8): 2801 - 2809.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
W. B. Stallcup and K. Dahlin-Huppe
Chondroitin sulfate and cytoplasmic domain-dependent membrane targeting of the NG2 proteoglycan promotes retraction fiber formation and cell polarization
J. Cell Sci., March 8, 2002; 114(12): 2315 - 2325.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Essler and E. Ruoslahti
Molecular specialization of breast vasculature: A breast-homing phage-displayed peptide binds to aminopeptidase P in breast vasculature
PNAS, February 19, 2002; 99(4): 2252 - 2257.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. Sundberg, J. A. Nagy, L. F. Brown, D. Feng, I. A. Eckelhoefer, E. J. Manseau, A. M. Dvorak, and H. F. Dvorak
Glomeruloid Microvascular Proliferation Follows Adenoviral Vascular Permeability Factor/Vascular Endothelial Growth Factor-164 Gene Delivery
Am. J. Pathol., March 1, 2001; 158(3): 1145 - 1160.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Pasqualini, E. Koivunen, R. Kain, J. Lahdenranta, M. Sakamoto, A. Stryhn, R. A. Ashmun, L. H. Shapiro, W. Arap, and E. Ruoslahti
Aminopeptidase N Is a Receptor for Tumor-homing Peptides and a Target for Inhibiting Angiogenesis
Cancer Res., February 1, 2000; 60(3): 722 - 727.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
L. Goretzki, C. R. Lombardo, and W. B. Stallcup
Binding of the NG2 Proteoglycan to Kringle Domains Modulates the Functional Properties of Angiostatin and Plasmin(ogen)
J. Biol. Chem., September 8, 2000; 275(37): 28625 - 28633.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 1999 by the American Association for Cancer Research.