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Rapid Isolation of Chromosomal Breakpoints from Patients with t(4;11) Acute Lymphoblastic Leukemia

Implications for Basic and Clinical Research¹

Chair of Genetics, University of Erlangen-Nürnberg, D-91058 Erlangen [M. R., E. G., I. B., G. H. F., R. M.], and Department of Pediatrics, University of Erlangen-Nürnberg, D-91054 Erlangen [R. R., J. G., J. D. B.], Germany

Chromosomal translocations t(4;11)(q21;q23) are associated with a group of acute lymphoblastic leukemias with very poor prognosis. From the complete sequences of the breakpoint cluster regions of the human MLL and AF-4 translocation partner genes, a novel set of 66 oligonucleotides that facilitates the rapid identification of translocation breakpoints by PCR analysis of genomic DNA was designed. For each breakpoint, a pair of optimally suited primers can be assigned, which improves the monitoring of the disease during treatment. Comparison of the breakpoints with the corresponding parental sequences also contributes to our better understanding of the illegitimate recombination events leading to these translocations.

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