This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jin, W. Articles by Cote, G. J. Search for Related Content PubMed PubMed Citation Articles by Jin, W. Articles by Cote, G. J.

Glioblastoma Cell-specific Expression of Fibroblast Growth Factor Receptor-1ß Requires an Intronic Repressor of RNA Splicing¹

Section of Endocrine Neoplasia and Hormonal Disorders, Department of Medical Specialties, The University of Texas, M. D. Anderson Cancer Center, Houston, Texas 77030

The fibroblast growth factor receptor-1 (FGFR-1) primary transcript is alternatively processed to produce receptors that vary in their ligand affinity and specificity. A high affinity form of this receptor—FGFR-1ß—that lacks the exon is observed on the neoplastic transformation of glial cells. In this study, we have identified a 62-bp sequence located 97 bp downstream from the exon that is required for the exclusion of this exon in a human glioblastoma cell line. Deletion or mutation of this sequence is sufficient to allow enhanced inclusion of the exon or a heterologous exon in glioblastoma cells. Therefore, it would appear that this sequence element plays a key role in the glioblastoma-specific splicing to form FGFR-1ß mRNA.

This article has been cited by other articles:

				R. Klinck, A. Bramard, L. Inkel, G. Dufresne-Martin, J. Gervais-Bird, R. Madden, E. R. Paquet, C. Koh, J. P. Venables, P. Prinos, et al. Multiple Alternative Splicing Markers for Ovarian Cancer Cancer Res., February 1, 2008; 68(3): 657 - 663. [Abstract] [Full Text] [PDF]

				M. Sironi, G. Menozzi, G. P. Comi, R. Cagliani, N. Bresolin, and U. Pozzoli Analysis of intronic conserved elements indicates that functional complexity might represent a major source of negative selection on non-coding sequences Hum. Mol. Genet., September 1, 2005; 14(17): 2533 - 2546. [Abstract] [Full Text] [PDF]

				I. E. McCutcheon, S. J. Hentschel, G. N. Fuller, W. Jin, and G. J. Cote Expression of the splicing regulator polypyrimidine tract-binding protein in normal and neoplastic brain Neuro-oncol, January 1, 2004; 6(1): 9 - 14. [Abstract] [PDF]

				T. A. Cooper Highlights of Alternative Splicing Regulation Session: Yes, No, Maybe--A History of Paradigm Shifts Sci. Signal., October 23, 2001; 2001(105): pe35 - pe35. [Abstract] [Full Text] [PDF]

				H. Lou and R. F. Gagel Alternative Ribonucleic Acid Processing in Endocrine Systems Endocr. Rev., April 1, 2001; 22(2): 205 - 225. [Abstract] [Full Text]

				B. H. Joshi, G. E. Plautz, and R. K. Puri Interleukin-13 Receptor {{alpha}} Chain: A Novel Tumor-associated Transmembrane Protein in Primary Explants of Human Malignant Gliomas Cancer Res., March 1, 2000; 60(5): 1168 - 1172. [Abstract] [Full Text]

				W. Jin, I. E. McCutcheon, G. N. Fuller, E. S-C. Huang, and G. J. Cote Fibroblast Growth Factor Receptor-1 {{alpha}}-Exon Exclusion and Polypyrimidine Tract-binding Protein in Glioblastoma Multiforme Tumors Cancer Res., March 1, 2000; 60(5): 1221 - 1224. [Abstract] [Full Text]

				W. Jin, E. S.-C. Huang, W. Bi, and G. J. Cote Redundant Intronic Repressors Function to Inhibit Fibroblast Growth Factor Receptor-1 alpha -Exon Recognition in Glioblastoma Cells J. Biol. Chem., September 24, 1999; 274(39): 28035 - 28041. [Abstract] [Full Text] [PDF]