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Comparative Genomic Hybridization and Loss of Heterozygosity Analyses Identify a Common Region of Deletion at 15q11.1–15 in Human Malignant Mesothelioma¹

Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 [B. R. B., D. W. B., G. S., A. D. R., J. R. T.]; Institut de Genetique et de Biologie Moleculaire et Cellulaire, Institut National de la Santé et de la Recherche Médicale, Strasbourg, France [S. d. M.]; and Department of Human Genetics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021 [S. C. J.]

Comparative genomic hybridization analysis was performed to identify chromosomal imbalances in 24 human malignant mesothelioma (MM) cell lines derived from untreated primary tumors. Chromosomal losses accounted for the majority of genomic imbalances. The most frequent underrepresented segments were 22q (58%) and 15q11.1–21 (54%); other recurrent sites of chromosomal loss included 1p12–22 (42%), 13q12–14 (42%), 14q24–qter (42%), 6q25–qter (38%), and 9p21 (38%). The most commonly overrepresented segment was 5p (54%). DNA sequence amplification at 3p12–13 was observed in two cases. Whereas some of the regions of copy number decreases (i.e., segments in 1p, 6q, 9p, and 22q) have previously been shown to be common sites of karyotypic and allelic loss in MM, our comparative genomic hybridization analyses identified a new recurrent site of chromosomal loss within 15q in this malignancy. To more precisely map the region of 15q deletion, loss of heterozygosity analyses were performed with a panel of polymorphic microsatellite markers distributed along 15q, which defined a minimal region of chromosomal loss at 15q11.1–15. The identification of frequent losses of a discrete segment in 15q suggests that this region harbors a putative tumor suppressor gene whose loss/inactivation may contribute to the pathogenesis of many MMs.

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