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Cell Cycle Regulation of Menin Expression¹

Departments of Medicine [H. K., L. C., D. G., G. N. H.], Physiology [D. G., G. N. H.], and Human Genetics [G. N. H.], McGill University, Montreal, Quebec, H3A 1A1 Canada

The multiple endocrine neoplasia type 1 gene product, menin, interacts with Jun D. The physiological role of menin in cell cycle control and the manner in which its inactivation contributes to tumorigenesis remain unknown. In the present study, the expression of menin was examined at various cell cycle stages in GH4C1 cells, a rat pituitary cell line. Cells synchronized at the G₁-S-phase boundary expressed menin at a lower level than G₀-G₁-synchronized cells. The expression of menin increased as the cells entered S phase, at which time Jun D expression also increased. In contrast, cells synchronized at the G₂-M phase expressed lower levels of menin. At G₀-G₁, G₁-S, and G₂-M phases of the cell cycle, menin was found predominantly in the nucleus. In summary, we show that in pituitary cells, menin is a nuclear protein whose expression is cell-cycle regulated. The data suggest that menin has an important role in cell growth regulation.

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