This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wiedle, G. Articles by Imhof, B. A. Search for Related Content PubMed PubMed Citation Articles by Wiedle, G. Articles by Imhof, B. A.

A Chimeric Cell Adhesion Molecule Mediates Homing of Lymphocytes to Vascularized Tumors¹

Department of Pathology, Centre Médical Universitaire, CH-1211 Geneva 4, Switzerland

To facilitate tumor colonization by adoptively transferred cells of the immune system, we created a chimeric cell adhesion molecule that mediates tumor-specific homing by binding to the integrin v3 on angiogenic endothelial cells. A high-affinity cell adhesion molecule for integrin v3 was generated by fusing the disintegrin kistrin to the transmembrane adhesion molecule CD31/PECAM-1. This chimeric cell adhesion molecule, termed KISS31, mediates adhesion of lymphoid cells to soluble recombinant integrin v3 and to endotheliomal monolayers in vitro. KISS31-expressing lymphoid cells accumulate in angiogenic tumors in two in vivo models, in B16/129 melanoma xenografts on the chick chorioallantois and in s.c. growing Lewis lung carcinoma in mice.

Our data indicate that expression of KISS31 on lymphoid cells confers tumor-specific homing. This is, to our knowledge, the first example of an experimental mechanism that targets living cells to tumors by redirecting their homing pattern.

This article has been cited by other articles:

				C. Lamagna, P. Meda, G. Mandicourt, J. Brown, R. J.C. Gilbert, E. Y. Jones, F. Kiefer, P. Ruga, B. A. Imhof, and M. Aurrand-Lions Dual Interaction of JAM-C with JAM-B and {alpha}M{beta}2 Integrin: Function in Junctional Complexes and Leukocyte Adhesion Mol. Biol. Cell, October 1, 2005; 16(10): 4992 - 5003. [Abstract] [Full Text] [PDF]

				M. Aurrand-Lions, C. Johnson-Leger, C. Wong, L. Du Pasquier, and B. A. Imhof Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members Blood, December 15, 2001; 98(13): 3699 - 3707. [Abstract] [Full Text] [PDF]

				D. Legler, G Wiedle, F. Ross, and B. Imhof Superactivation of integrin (&agr;)v(&bgr;)3 by low antagonist concentrations J. Cell Sci., January 4, 2001; 114(8): 1545 - 1553. [Abstract] [PDF]

				M. Aurrand-Lions, L. Duncan, C. Ballestrem, and B. A. Imhof JAM-2, a Novel Immunoglobulin Superfamily Molecule, Expressed by Endothelial and Lymphatic Cells J. Biol. Chem., January 19, 2001; 276(4): 2733 - 2741. [Abstract] [Full Text] [PDF]