Cancer Research Prevention Award Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
[Cancer Research 59, 5554-5559, November 1, 1999]
© 1999 American Association for Cancer Research
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vissers, J. L. M.
Right arrow Articles by Adema, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vissers, J. L. M.
Right arrow Articles by Adema, G. J.
[Cancer Research 59, 5554-5559, November 1, 1999]
© 1999 American Association for Cancer Research

Immunology

The Renal Cell Carcinoma-associated Antigen G250 Encodes a Human Leukocyte Antigen (HLA)-A2.1-restricted Epitope Recognized by Cytotoxic T Lymphocytes1

Joost L. M. Vissers, I. Jolanda M. De Vries, Marco W. J. Schreurs, Linda P. H. Engelen, Egbert Oosterwijk, Carl G. Figdor and Gosse J. Adema2

Tumor Immunology Laboratory [J. L. M. V., I. J. M. D. V., M. W. J. S., L. P. H. E., C. G. F., G. J. A.] and Department of Urology [E. O.], University Hospital Nijmegen St. Radboud, 6525 EX Nijmegen, the Netherlands

Evidence has accumulated that the immune system can play a significant role in the defense against tumors in humans. Especially melanoma and renal cell carcinoma (RCC) are considered immunogenic tumors. In contrast to melanoma, hardly any RCC-associated antigens have been identified as targets for RCC-reactive T cells. Here, we report the identification of a human leukocyte antigen (HLA)-A2.1-restricted T-cell epitope within the G250 antigen. This antigen is expressed in 85% of RCCs but not by neighboring normal kidney tissue and has recently been molecularly defined and shown to be identical to MN/CA IX. Computer-aided motif prediction revealed the presence of 60 potential HLA-A2.1-binding peptides within the G250 antigen. Subsequent binding analysis showed that 13 of these peptides bound to HLA-A2.1 with high-to-intermediate affinity. Analysis of their immunogenicity in HLA-A2.1Kb transgenic mice indicated that 4 of the 13 peptides gave rise to cytotoxic T lymphocytes (CTLs) capable of lysing peptide-loaded target cells. However, only the G250 peptide 254–262 induced CTLs that recognized target cells that endogenously expressed the G250 antigen. Similarly, we were also able to raise human CTLs against the G250 peptide 254–262, which lysed target cells that endogenously expressed the G250 antigen. These findings and the high prevalence of this antigen in RCC patients makes G250 a potential target for anti-RCC immunotherapy.




This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
J. Greiner, L. Bullinger, B.-a. Guinn, H. Dohner, and M. Schmitt
Leukemia-Associated Antigens Are Critical for the Proliferation of Acute Myeloid Leukemia Cells
Clin. Cancer Res., November 15, 2008; 14(22): 7161 - 7166.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Greiner, M. Schmitt, L. Li, K. Giannopoulos, K. Bosch, A. Schmitt, K. Dohner, R. F. Schlenk, J. R. Pollack, H. Dohner, et al.
Expression of tumor-associated antigens in acute myeloid leukemia: implications for specific immunotherapeutic approaches
Blood, December 15, 2006; 108(13): 4109 - 4117.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Kausche, T. Wehler, E. Schnurer, V. Lennerz, W. Brenner, S. Melchior, M. Grone, M. Nonn, S. Strand, R. Meyer, et al.
Superior Antitumor In vitro Responses of Allogeneic Matched Sibling Compared with Autologous Patient CD8+ T Cells
Cancer Res., December 1, 2006; 66(23): 11447 - 11454.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
E. Tassi, V. Facchinetti, S. Seresini, A. Borri, G. Dell'Antonio, C. Garavaglia, G. Casorati, and M. P. Protti
Peptidome from Renal Cell Carcinoma Contains Antigens Recognized by CD4+ T Cells and Shared among Tumors of Different Histology.
Clin. Cancer Res., August 15, 2006; 12(16): 4949 - 4957.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. Zhou, D. Y. Jun, A. M. Thomas, X. Huang, L.-Q. Huang, J. Mautner, W. Mo, P. F. Robbins, D. M. Pardoll, and E. M. Jaffee
Diverse CD8+ T-Cell Responses to Renal Cell Carcinoma Antigens in Patients Treated with an Autologous Granulocyte-Macrophage Colony-Stimulating Factor Gene-Transduced Renal Tumor Cell Vaccine
Cancer Res., February 1, 2005; 65(3): 1079 - 1088.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
N. Uchida, T. Tsunoda, S. Wada, Y. Furukawa, Y. Nakamura, and H. Tahara
Ring Finger Protein 43 as a New Target for Cancer Immunotherapy
Clin. Cancer Res., December 15, 2004; 10(24): 8577 - 8586.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
D. Avigan
Dendritic Cell-Tumor Fusion Vaccines for Renal Cell Carcinoma
Clin. Cancer Res., September 15, 2004; 10(18): 6347S - 6352S.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. R. Parkhurst, J. P. Riley, T. Igarashi, Y. Li, P. F. Robbins, and S. A. Rosenberg
Immunization of Patients with the hTERT:540-548 Peptide Induces Peptide-Reactive T Lymphocytes That Do Not Recognize Tumors Endogenously Expressing Telomerase
Clin. Cancer Res., July 15, 2004; 10(14): 4688 - 4698.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Wang, H. Chen, X. Jiang, M. Zhang, T. Wan, N. Li, X. Zhou, Y. Wu, F. Yang, Y. Yu, et al.
Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein
Blood, July 1, 2004; 104(1): 200 - 206.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
H. Mukouyama, N. K. Janzen, J. M. Hernandez, J. S. Lam, R. Caliliw, A. Y. Wang, R. A. Figlin, A. S. Belldegrun, and G. Zeng
Generation of Kidney Cancer-Specific Antitumor Immune Responses Using Peripheral Blood Monocytes Transduced With a Recombinant Adenovirus Encoding Carbonic Anhydrase 9
Clin. Cancer Res., February 15, 2004; 10(4): 1421 - 1429.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. J. Pantuck, G. Zeng, A. S. Belldegrun, and R. A. Figlin
Pathobiology, Prognosis, and Targeted Therapy for Renal Cell Carcinoma: Exploiting the Hypoxia-Induced Pathway
Clin. Cancer Res., October 15, 2003; 9(13): 4641 - 4652.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. M. Hernandez, M. H. T. Bui, K.-r. Han, H. Mukouyama, D. G. Freitas, D. Nguyen, R. Caliliw, P. I. Shintaku, S. H. Paik, C.-L. Tso, et al.
Novel Kidney Cancer Immunotherapy Based on the Granulocyte- Macrophage Colony-stimulating Factor and Carbonic Anhydrase IX Fusion Gene
Clin. Cancer Res., May 1, 2003; 9(5): 1906 - 1916.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. H. T. Bui, D. Seligson, K.-r. Han, A. J. Pantuck, F. J. Dorey, Y. Huang, S. Horvath, B. C. Leibovich, S. Chopra, S.-Y. Liao, et al.
Carbonic Anhydrase IX Is an Independent Predictor of Survival in Advanced Renal Clear Cell Carcinoma: Implications for Prognosis and Therapy
Clin. Cancer Res., February 1, 2003; 9(2): 802 - 811.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Weinschenk, C. Gouttefangeas, M. Schirle, F. Obermayr, S. Walter, O. Schoor, R. Kurek, W. Loeser, K.-H. Bichler, D. Wernet, et al.
Integrated Functional Genomics Approach for the Design of Patient-individual Antitumor Vaccines
Cancer Res., October 15, 2002; 62(20): 5818 - 5827.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. M. Krackhardt, M. Witzens, S. Harig, F. S. Hodi, A. J. Zauls, M. Chessia, P. Barrett, and J. G. Gribben
Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX
Blood, August 28, 2002; 100(6): 2123 - 2131.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C.-L. Tso, A. Zisman, A. Pantuck, R. Calilliw, J. M. Hernandez, S. Paik, D. Nguyen, B. Gitlitz, P. I. Shintaku, J. de Kernion, et al.
Induction of G250-targeted and T-Cell-mediated Antitumor Activity against Renal Cell Carcinoma Using a Chimeric Fusion Protein Consisting of G250 and Granulocyte/Monocyte-Colony Stimulating Factor
Cancer Res., November 1, 2001; 61(21): 7925 - 7933.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K.-i. Hanada, D. M. Perry-Lalley, G. A. Ohnmacht, M. P. Bettinotti, and J. C. Yang
Identification of Fibroblast Growth Factor-5 as an Overexpressed Antigen in Multiple Human Adenocarcinomas
Cancer Res., July 1, 2001; 61(14): 5511 - 5516.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
G. Li, K. Passebosc-Faure, C. Lambert, A. Gentil-Perret, F. Blanc, E. Oosterwijk, J.-F. Mosnier, C. Genin, and J. Tostain
The Expression of G250/MN/CA9 Antigen by Flow Cytometry: Its Possible Implication for Detection of Micrometastatic Renal Cancer Cells
Clin. Cancer Res., January 1, 2001; 7(1): 89 - 92.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
M. W. J. Schreurs, A. A. O. Eggert, A. J. de Boer, J. L. M. Vissers, T. van Hall, R. Offringa, C. G. Figdor, and G. J. Adema
Dendritic Cells Break Tolerance and Induce Protective Immunity against a Melanocyte Differentiation Antigen in an Autologous Melanoma Model
Cancer Res., December 1, 2000; 60(24): 6995 - 7001.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 1999 by the American Association for Cancer Research.