| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Tumor Biology |
Division of Nutrition [W. Y., K. I., Q. Y. L., K. K.] and Department of Zoology [W. Y., Q. Y. L., B. G. S.], University of Texas at Austin, Austin, Texas 78712; and The University of Texas M. D. Anderson Cancer Center, Science Park-Research Division, Smithville, Texas 78957 [C. M. A.]
Fas (CD95/APO-1) is an important mediator of apoptosis. We show that Fas-resistant MCF-7, MDA-MB-231, and MDA-MB-435 human breast cancer cells become responsive to anti-Fas (CD95) agonistic antibody-triggered apoptosis after pretreatment or cotreatment with vitamin E succinate (VES; RRR-
-tocopheryl succinate). In contrast, no enhancement of anti-Fas agonistic antibody-triggered apoptosis was observed following VES pretreatment or cotreatment with Fas-sensitive primary cultures of human mammary epithelial cells, immortalized MCF-10A cells, or T47D human breast cancer cells. Although VES is itself a potent apoptotic triggering agent, the 6-h pretreatment procedure for Fas sensitization did not initiate VES-mediated apoptosis. The combination of VES plus anti-Fas in pretreatment protocols was synergistic, inducing 2.8-, 3.0-, and 6.3-fold enhanced apoptosis in Fas-resistant MCF-7, MDA-MB-231, and MDA-MB-435 cells, respectively. Likewise, cotreatment of Fas-resistant MCF-7, MDA-MB-231, and MDA-MB-435 cells with VES plus anti-Fas enhanced apoptosis 1.9-, 2.0-, and 2.6-fold, respectively. Functional knockout of Fas-mediated signaling with either Fas-neutralizing antibody (MCF-7-, MDA-MB-231-, and MDA-MB-435-treated cells) or Fas antisense oligomers (MDA-MB-435-treated cells only), reduced VES-triggered apoptosis by 
50%. Analyses of whole cell extracts from Fas-sensitive cells revealed high constitutive expression of Mr 43,000 Fas, whereas Fas-resistant cells expressed low levels that were confined to the cytosolic fraction. VES treatment of the Fas-resistant cells caused a depletion of cytosolic Mr 43,000 Fas with a concomitant increase in Mr 43,000 membrane Fas. These data show that VES can convert Fas-resistant human breast cancer cells to a Fas-sensitive phenotype, perhaps by translocation of cytosolic Mr 43,000 Fas to the membrane and show that VES-mediated apoptosis involves Mr 43,000 Fas signaling.
This article has been cited by other articles:
|
|
 |
|
  M. Shim and T. E. Eling Vitamin E succinate induces NAG-1 expression in a p38 kinase-dependent mechanism Mol. Cancer Ther., April 1, 2008; 7(4): 961 - 971. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Neuzil, M. Tomasetti, Y. Zhao, L.-F. Dong, M. Birringer, X.-F. Wang, P. Low, K. Wu, B. A. Salvatore, and S. J. Ralph Vitamin E Analogs, a Novel Group of "Mitocans," as Anticancer Agents: The Importance of Being Redox-Silent Mol. Pharmacol., May 1, 2007; 71(5): 1185 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yin, J. Ni, M. Chen, M. A. DiMaggio, Y. Guo, and S. Yeh The Therapeutic and Preventive Effect of RRR-{alpha}-Vitamin E Succinate on Prostate Cancer via Induction of Insulin-Like Growth Factor Binding Protein-3 Clin. Cancer Res., April 1, 2007; 13(7): 2271 - 2280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Donapaty, S. Louis, E. Horvath, J. Kun, S. M. Sebti, and M. P. Malafa RRR-{alpha}-Tocopherol succinate down-regulates oncogenic Ras signaling. Mol. Cancer Ther., February 1, 2006; 5(2): 309 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kline, W. Yu, and B. G. Sanders Vitamin E and Breast Cancer J. Nutr., December 1, 2004; 134(12): 3458S - 3462S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Adams and D. Teegarden 1,25-Dihydroxycholecalciferol Inhibits Apoptosis in C3H10T1/2 Murine Fibroblast Cells Through Activation of Nuclear Factor {kappa}B J. Nutr., November 1, 2004; 134(11): 2948 - 2952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Lawson, K. Anderson, M. Simmons-Menchaca, J. Atkinson, L. Sun, B. G. Sanders, and K. Kline Comparison of Vitamin E Derivatives {alpha}-TEA and VES in Reduction of Mouse Mammary Tumor Burden and Metastasis Experimental Biology and Medicine, October 1, 2004; 229(9): 954 - 963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shah and P. W. Sylvester Tocotrienol-Induced Caspase-8 Activation Is Unrelated to Death Receptor Apoptotic Signaling in Neoplastic Mammary Epithelial Cells Experimental Biology and Medicine, September 1, 2004; 229(8): 745 - 755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Anderson, M. Simmons-Menchaca, K. A. Lawson, J. Atkinson, B. G. Sanders, and K. Kline Differential Response of Human Ovarian Cancer Cells to Induction of Apoptosis by Vitamin E Succinate and Vitamin E Analogue, {alpha}-TEA Cancer Res., June 15, 2004; 64(12): 4263 - 4269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Zu and C. Ip Synergy between Selenium and Vitamin E in Apoptosis Induction Is Associated with Activation of Distinctive Initiator Caspases in Human Prostate Cancer Cells Cancer Res., October 15, 2003; 63(20): 6988 - 6995. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bradford, J. Atkinson, N. Fuller, and R. P. Rand The effect of vitamin E on the structure of membrane lipid assemblies J. Lipid Res., October 1, 2003; 44(10): 1940 - 1945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yu, B. G. Sanders, and K. Kline RRR-{alpha}-Tocopheryl Succinate-induced Apoptosis of Human Breast Cancer Cells Involves Bax Translocation to Mitochondria Cancer Res., May 15, 2003; 63(10): 2483 - 2491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Lawson, K. Anderson, M. Menchaca, J. Atkinson, L. Sun, V. Knight, B. E. Gilbert, C. Conti, B. G. Sanders, and K. Kline Novel Vitamin E Analogue Decreases Syngeneic Mouse Mammary Tumor Burden and Reduces Lung Metastasis Mol. Cancer Ther., May 1, 2003; 2(5): 437 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. N. Prasad, B. Kumar, X.-D. Yan, A. J. Hanson, and W. C. Cole {alpha}-Tocopheryl Succinate, the Most Effective Form of Vitamin E for Adjuvant Cancer Treatment: A Review J. Am. Coll. Nutr., April 1, 2003; 22(2): 108 - 117. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Brigelius-Flohe, F. J Kelly, J. T Salonen, J. Neuzil, J.-M. Zingg, and A. Azzi The European perspective on vitamin E: current knowledge and future research Am. J. Clinical Nutrition, October 1, 2002; 76(4): 703 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Kumar, M. N. Jha, W. C. Cole, J. S. Bedford, and K. N. Prasad D-Alpha-Tocopheryl Succinate (Vitamin E) Enhances Radiation-Induced Chromosomal Damage Levels in Human Cancer Cells, but Reduces it in Normal Cells J. Am. Coll. Nutr., August 1, 2002; 21(4): 339 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Weber, M. Lu, L. Andera, H. Lahm, N. Gellert, M. W. Fariss, V. Korinek, W. Sattler, D. S. Ucker, A. Terman, et al. Vitamin E Succinate Is a Potent Novel Antineoplastic Agent with High Selectivity and Cooperativity with Tumor Necrosis Factor-related Apoptosis-inducing Ligand (Apo2 Ligand) in Vivo Clin. Cancer Res., March 1, 2002; 8(3): 863 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. N. Prasad, W. C. Cole, B. Kumar, and K. C. Prasad Scientific Rationale for Using High-Dose Multiple Micronutrients as an Adjunct to Standard and Experimental Cancer Therapies J. Am. Coll. Nutr., October 1, 2001; 20(90005): 450S - 463. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. You, W. Yu, B. G. Sanders, and K. Kline RRR-{alpha}-Tocopheryl Succinate Induces MDA-MB-435 and MCF-7 Human Breast Cancer Cells to Undergo Differentiation Cell Growth Differ., September 1, 2001; 12(9): 471 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yu, Q. Y. Liao, F. M. Hantash, B. G. Sanders, and K. Kline Activation of Extracellular Signal-regulated Kinase and c-Jun-NH2-terminal Kinase but not p38 Mitogen-activated Protein Kinases Is Required for RRR-{alpha}-Tocopheryl Succinate-induced Apoptosis of Human Breast Cancer Cells Cancer Res., September 1, 2001; 61(17): 6569 - 6576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kline, W. Yu, and B. G. Sanders Vitamin E: Mechanisms of Action as Tumor Cell Growth Inhibitors J. Nutr., January 1, 2001; 131(1): 161S - 163. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
