This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shestakova, E. A. Articles by Condeelis, J. Search for Related Content PubMed PubMed Citation Articles by Shestakova, E. A. Articles by Condeelis, J.

Correlation of ß-Actin Messenger RNA Localization with Metastatic Potential in Rat Adenocarcinoma Cell Lines¹

Departments of Anatomy and Structural Biology [E. A. S., J. W., R. H. S., J. C.] and Pathology [J. J.], Albert Einstein College of Medicine, Bronx, New York 10461

The actin cytoskeleton is involved in the motility of tumor cells. It has been shown in several cell types that ß-actin mRNA is localized in the protrusions of cells in which actin is actively polymerized, and the ability to localize mRNA is correlated with the efficiency of motility. In this context, we studied the distribution of ß-actin mRNA in two different tumor cell lines and correlated it with their metastatic potential. The two cell lines used were the highly metastatic MTLn3 cells and nonmetastatic MTC cells. Nonmetastatic MTC cells have two different pools of ß-actin mRNA (perinuclear and at the leading edge), whereas highly metastatic MTLn3 cells have only a perinuclear distribution of ß-actin mRNA. These differences in mRNA localization are correlated with profound differences in the polarity and plasticity of cell motility of these cells in culture and the histopathology of primary breast tumors derived from these cells. In particular, MTLn3 cells are unpolarized by all cell shape and motility criteria in culture and in their histopathological organization in primary tumors. By comparison, MTC cells are polarized in all identical measurements. These results suggest that the increased plasticity of cell locomotion and the invasiveness of MTLn3 cells result from the failure of metastatic cells to localize ß-actin mRNA properly, causing them to be less polarized and therefore more flexible in their direction of motility. Thus, differences in the polarized organization of cells in the primary tumor that are correlated with ß-actin mRNA localization may have prognostic value in predicting metastatic potential.

This article has been cited by other articles:

				M. Sidani, D. Wessels, G. Mouneimne, M. Ghosh, S. Goswami, C. Sarmiento, W. Wang, S. Kuhl, M. El-Sibai, J. M. Backer, et al. Cofilin determines the migration behavior and turning frequency of metastatic cancer cells J. Cell Biol., November 19, 2007; 179(4): 777 - 791. [Abstract] [Full Text] [PDF]

				K. Lapidus, J. Wyckoff, G. Mouneimne, M. Lorenz, L. Soon, J. S. Condeelis, and R. H. Singer ZBP1 enhances cell polarity and reduces chemotaxis J. Cell Sci., September 15, 2007; 120(18): 3173 - 3178. [Abstract] [Full Text] [PDF]

				Q. Luo, L. Siconolfi-Baez, P. Annamaneni, M. T. Bielawski, P. M. Novikoff, and R. H. Angeletti Altered protein expression at early-stage rat hepatic neoplasia Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1272 - G1282. [Abstract] [Full Text] [PDF]

				A. J. Rodriguez, S. M. Shenoy, R. H. Singer, and J. Condeelis Visualization of mRNA translation in living cells J. Cell Biol., October 9, 2006; 175(1): 67 - 76. [Abstract] [Full Text] [PDF]

				W. Wang, S. Goswami, K. Lapidus, A. L. Wells, J. B. Wyckoff, E. Sahai, R. H. Singer, J. E. Segall, and J. S. Condeelis Identification and Testing of a Gene Expression Signature of Invasive Carcinoma Cells within Primary Mammary Tumors Cancer Res., December 1, 2004; 64(23): 8585 - 8594. [Abstract] [Full Text] [PDF]

				M. Ghosh, X. Song, G. Mouneimne, M. Sidani, D. S. Lawrence, and J. S. Condeelis Cofilin Promotes Actin Polymerization and Defines the Direction of Cell Motility Science, April 30, 2004; 304(5671): 743 - 746. [Abstract] [Full Text] [PDF]

				K. L. Farina, S. Huttelmaier, K. Musunuru, R. Darnell, and R. H. Singer Two ZBP1 KH domains facilitate {beta}-actin mRNA localization, granule formation, and cytoskeletal attachment J. Cell Biol., January 2, 2003; 160(1): 77 - 87. [Abstract] [Full Text] [PDF]

				W. Wang, J. B. Wyckoff, V. C. Frohlich, Y. Oleynikov, S. Huttelmaier, J. Zavadil, L. Cermak, E. P. Bottinger, R. H. Singer, J. G. White, et al. Single Cell Behavior in Metastatic Primary Mammary Tumors Correlated with Gene Expression Patterns Revealed by Molecular Profiling Cancer Res., November 1, 2002; 62(21): 6278 - 6288. [Abstract] [Full Text] [PDF]

				V. DesMarais, I. Ichetovkin, J. Condeelis, and S. E. Hitchcock-DeGregori Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge J. Cell Sci., January 12, 2002; 115(23): 4649 - 4660. [Abstract] [Full Text] [PDF]

				N. Zebda, O. Bernard, M. Bailly, S. Welti, D. S. Lawrence, and J. S. Condeelis Phosphorylation of ADF/Cofilin Abolishes EGF-induced Actin Nucleation at the Leading Edge and Subsequent Lamellipod Extension J. Cell Biol., November 20, 2000; 151(5): 1119 - 1128. [Abstract] [Full Text] [PDF]

				J. B. Wyckoff, J. E. Segall, and J. S. Condeelis The Collection of the Motile Population of Cells from a Living Tumor Cancer Res., October 1, 2000; 60(19): 5401 - 5404. [Abstract] [Full Text]

				J. B. Wyckoff, J. G. Jones, J. S. Condeelis, and J. E. Segall A Critical Step in Metastasis: In Vivo Analysis of Intravasation at the Primary Tumor Cancer Res., May 1, 2000; 60(9): 2504 - 2511. [Abstract] [Full Text]

				A. Chan, M Kloc, and L. Etkin fatvg encodes a new localized RNA that uses a 25-nucleotide element (FVLE1) to localize to the vegetal cortex of Xenopus oocytes Development, January 11, 1999; 126(22): 4943 - 4953. [Abstract] [PDF]