| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biochemistry |
Department of Internal Medicine I [H. B., I. H. E., K. S-O., S. W.] and Department of Physical Biochemistry of the Institute of Physiological Chemistry [W. V.], Eberhard-Karls-University, D-72076 Tübingen, Germany
Mistletoe lectin I (ML-I) is a major active component in plant extracts of Viscum album that is increasingly used in adjuvant cancer therapy. ML-I exerts potent immunomodulating and cytotoxic effects, although its mechanism of action is largely unknown. We show that treatment of leukemic T- and B-cell lines with ML-I induced apoptosis, which required the prior activation of proteases of the caspase family. The involvement of caspases is demonstrated because (a) a peptide caspase inhibitor almost completely prevented ML-I-induced cell death and (b) proteolytic activation of caspase-8, caspase-9, and caspase-3 was observed. Because caspase-8 has been implicated as a regulator of apoptosis mediated by death receptors, we further investigated a potential receptor involvement in ML-I-induced effects. Cell death triggered by ML-I was neither attenuated in cell clones resistant to CD95 nor in cells that were rendered refractory to other death receptors by overexpressing a dominant-negative FADD mutant. In contrast, ML-I triggered a receptor-independent mitochondria-controlled apoptotic pathway because it rapidly induced the release of cytochrome c into the cytosol. Because ML-I was also observed to enhance the cytotoxic effect of chemotherapeutic drugs, these data may provide a molecular basis for clinical trials using MLs in anticancer therapy.
This article has been cited by other articles:
|
|
 |
|
  A. Bagaria, K. Surendranath, U. A. Ramagopal, S. Ramakumar, and A. A. Karande Structure-Function Analysis and Insights into the Reduced Toxicity of Abrus precatorius Agglutinin I in Relation to Abrin J. Biol. Chem., November 10, 2006; 281(45): 34465 - 34474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nencioni, F. Hua, C. P. Dillon, R. Yokoo, C. Scheiermann, M. H. Cardone, E. Barbieri, I. Rocco, A. Garuti, S. Wesselborg, et al. Evidence for a protective role of Mcl-1 in proteasome inhibitor-induced apoptosis Blood, April 15, 2005; 105(8): 3255 - 3262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hajto, K. Hostanska, T. Berki, L. Palinkas, F. Boldizsar, and P. Nemeth Oncopharmacological Perspectives of a Plant Lectin (Viscum album Agglutinin-I): Overview of Recent Results from In vitro Experiments and In vivo Animal Models, and Their Possible Relevance for Clinical Applications Evid. Based Complement. Altern. Med., March 1, 2005; 2(1): 59 - 67. [Full Text] [PDF]
|
|
|
|
 |
|
 P. Schoffski, S. Riggert, P. Fumoleau, M. Campone, O. Bolte, S. Marreaud, D. Lacombe, B. Baron, M. Herold, H. Zwierzina, et al. Phase I trial of intravenous aviscumine (rViscumin) in patients with solid tumors: a study of the European Organization for Research and Treatment of Cancer New Drug Development Group Ann. Onc., December 1, 2004; 15(12): 1816 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-H. Kim, W. B. Park, B. Gao, and M. H. Jung Critical Role of Reactive Oxygen Species and Mitochondrial Membrane Potential in Korean Mistletoe Lectin-Induced Apoptosis in Human Hepatocarcinoma Cells Mol. Pharmacol., December 1, 2004; 66(6): 1383 - 1396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Neumeister, M. Faigle, K. Lauber, H. Northoff, and S. Wesselborg Legionella pneumophila induces apoptosis via the mitochondrial death pathway Microbiology, November 1, 2002; 148(11): 3639 - 3650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Varghese, H. Sade, P. Vandenabeele, and A. Sarin Head Involution Defective (Hid)-triggered Apoptosis Requires Caspase-8 but Not FADD (Fas-associated Death Domain) and Is Regulated by Erk in Mammalian Cells J. Biol. Chem., September 13, 2002; 277(38): 35097 - 35104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Lavastre, M. Pelletier, R. Saller, K. Hostanska, and D. Girard Mechanisms Involved in Spontaneous and Viscum album Agglutinin-I-Induced Human Neutrophil Apoptosis: Viscum album Agglutinin-I Accelerates the Loss of Antiapoptotic Mcl-1 Expression and the Degradation of Cytoskeletal Paxillin and Vimentin Proteins Via Caspases J. Immunol., February 1, 2002; 168(3): 1419 - 1427. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chatterjee, I. Schmitz, A. Krueger, K. Yeung, S. Kirchhoff, P. H. Krammer, M. E. Peter, J. H. Wyche, and P. Pantazis Induction of Apoptosis in 9-Nitrocamptothecin-treated DU145 Human Prostate Carcinoma Cells Correlates with de Novo Synthesis of CD95 and CD95 Ligand and Down-Regulation of c-FLIPshort Cancer Res., October 1, 2001; 61(19): 7148 - 7154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-M. Boneberg and T. Hartung Mistletoe Lectin-1 Increases Tumor Necrosis Factor-alpha Release in Lipopolysaccharide-Stimulated Whole Blood via Inhibition of Interleukin-10 Production J. Pharmacol. Exp. Ther., September 1, 2001; 298(3): 996 - 1000. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Kurita-Ochiai, K. Ochiai, and K. Fukushima Butyric Acid-Induced T-Cell Apoptosis Is Mediated by Caspase-8 and -9 Activation in a Fas-Independent Manner Clin. Vaccine Immunol., March 1, 2001; 8(2): 325 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wieder, F. Essmann, A. Prokop, K. Schmelz, K. Schulze-Osthoff, R. Beyaert, B. Dorken, and P. T. Daniel Activation of caspase-8 in drug-induced apoptosis of B-lymphoid cells is independent of CD95/Fas receptor-ligand interaction and occurs downstream of caspase-3 Blood, March 1, 2001; 97(5): 1378 - 1387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Inman and M. J. Allday Apoptosis Induced by TGF-{beta}1 in Burkitt's Lymphoma Cells Is Caspase 8 Dependent But Is Death Receptor Independent J. Immunol., September 1, 2000; 165(5): 2500 - 2510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Walczak, A. Bouchon, H. Stahl, and P. H. Krammer Tumor Necrosis Factor-related Apoptosis-inducing Ligand Retains Its Apoptosis-inducing Capacity on Bcl-2- or Bcl-xL-overexpressing Chemotherapy-resistant Tumor Cells Cancer Res., June 1, 2000; 60(11): 3051 - 3057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W Liu, M Kato, A. Akhand, A Hayakawa, H Suzuki, T Miyata, K Kurokawa, Y Hotta, N Ishikawa, and I Nakashima 4-hydroxynonenal induces a cellular redox status-related activation of the caspase cascade for apoptotic cell death J. Cell Sci., January 2, 2000; 113(4): 635 - 641. [Abstract] [PDF]
|
|
|
|
|
|
K. Lauber, H. A. E. Appel, S. F. Schlosser, M. Gregor, K. Schulze-Osthoff, and S. Wesselborg The Adapter Protein Apoptotic Protease-activating Factor-1 (Apaf-1) Is Proteolytically Processed during Apoptosis J. Biol. Chem., August 3, 2001; 276(32): 29772 - 29781. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
