| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Virology |
Department of Pathology, Section Molecular Pathology [A. z. H., A. A. T. P. B, J. M. M., C. J. L. M. M., A. J. C. v. d. B.], and Department of Gastroenterology [M. E. C.], University Hospital Vrije Universiteit, 1007 MB Amsterdam, the Netherlands
Approximately 10% of gastric adenocarcinomas worldwide are associated with human EBV. These carcinomas generally do not express the latent membrane protein 1 (LMP1), the major known EBV oncogene.
Recently, another EBV gene [i.e., BARF1 (BamHI A rightward open reading frame)] was shown to have transforming and immortalizing capacities. Therefore, in this study, we investigated the expression of BARF1 in EBV-carrying gastric adenocarcinomas in relation to the expression of other latent EBV transcripts.
In the present study, 10 of 132 gastric adenocarcinomas tested positive for EBV using EBER1/2-RNA in situ hybridization. We demonstrate BARF1 gene transcription in nine EBV-carrying gastric adenocarcinomas (with sufficient RNA quality) using the BARF1-specific nucleic acid sequence-based amplification assay. In addition, we also detected other latent EBV transcripts (i.e., BARF0-, LMP2A-, and Q/K-driven EBNA1 transcripts in these carcinomas using reverse transcription-PCR analysis. No expression of LMP1, EBNA2, and ZEBRA (either at transcription or protein level) was found. In addition, two cases were positive for BHRF1 transcripts, the viral bcl-2 homologue. Thus, together with BARF1 transcription, a unique and distinct EBV latency type has been found in EBV-associated gastric adenocarcinomas.
Because BARF1 exerts immortalizing effects on human epithelial cells in vitro and EBV-carrying gastric adenocarcinomas lack the expression of LMP1, the BARF1 gene might act as the viral oncogene in EBV-carrying gastric carcinomas. The BARF1 gene offers an alternative way for EBV-mediated oncogenesis other than LMP1.
This article has been cited by other articles:
|
|
 |
|
  M. L. Gulley and W. Tang Laboratory Assays for Epstein-Barr Virus-Related Disease J. Mol. Diagn., July 1, 2008; 10(4): 279 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Hino, H. Uozaki, Y. Inoue, Y. Shintani, T. Ushiku, T. Sakatani, K. Takada, and M. Fukayama Survival Advantage of EBV-Associated Gastric Carcinoma: Survivin Up-regulation by Viral Latent Membrane Protein 2A Cancer Res., March 1, 2008; 68(5): 1427 - 1435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H-J Delecluse, R Feederle, B O'Sullivan, and P Taniere Epstein Barr virus-associated tumours: an update for the attention of the working pathologist J. Clin. Pathol., December 1, 2007; 60(12): 1358 - 1364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. de Turenne-Tessier and T. Ooka Post-translational modifications of Epstein Barr virus BARF1 oncogene-encoded polypeptide J. Gen. Virol., October 1, 2007; 88(10): 2656 - 2661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Houali, X. Wang, Y. Shimizu, D. Djennaoui, J. Nicholls, S. Fiorini, A. Bouguermouh, and T. Ooka A New Diagnostic Marker for Secreted Epstein-Barr Virus Encoded LMP1 and BARF1 Oncoproteins in the Serum and Saliva of Patients with Nasopharyngeal Carcinoma Clin. Cancer Res., September 1, 2007; 13(17): 4993 - 5000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. C. Stevens, S. A. W. M. Verkuijlen, B. Hariwiyanto, Harijadi, J. Fachiroh, D. K. Paramita, I. B. Tan, S. M. Haryana, and J. M. Middeldorp Diagnostic Value of Measuring Epstein-Barr Virus (EBV) DNA Load and Carcinoma-Specific Viral mRNA in Relation to Anti-EBV Immunoglobulin A (IgA) and IgG Antibody Levels in Blood of Nasopharyngeal Carcinoma Patients from Indonesia J. Clin. Microbiol., July 1, 2005; 43(7): 3066 - 3073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A zur Hausen, B P van Rees, J van Beek, M E Craanen, E Bloemena, G J A Offerhaus, C J L M Meijer, and A J C van den Brule Epstein-Barr virus in gastric carcinomas and gastric stump carcinomas: a late event in gastric carcinogenesis J. Clin. Pathol., May 1, 2004; 57(5): 487 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. van Beek, A. zur Hausen, E. Klein Kranenbarg, C. J.H. van de Velde, J. M. Middeldorp, A. J.C. van den Brule, C. J.L.M. Meijer, and E. Bloemena EBV-Positive Gastric Adenocarcinomas: A Distinct Clinicopathologic Entity With a Low Frequency of Lymph Node Involvement J. Clin. Oncol., February 15, 2004; 22(4): 664 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. zur Hausen, J. van Beek, E. Bloemena, F. J. ten Kate, C. J. L. M. Meijer, and A. J. C. van den Brule No role for Epstein-Barr virus in Dutch hepatocellular carcinoma: a study at the DNA, RNA and protein levels J. Gen. Virol., July 1, 2003; 84(7): 1863 - 1869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. van Rees, E. Caspers, A. zur Hausen, A. van den Brule, P. Drillenburg, M. A. J. Weterman, and G. J. A. Offerhaus Different Pattern of Allelic Loss in Epstein-Barr Virus-Positive Gastric Cancer with Emphasis on the p53 Tumor Suppressor Pathway Am. J. Pathol., October 1, 2002; 161(4): 1207 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q N Vo, J Geradts, M L Gulley, D A Boudreau, J C Bravo, and B G Schneider Epstein-Barr virus in gastric adenocarcinomas: association with ethnicity and CDKN2A promoter methylation J. Clin. Pathol., September 1, 2002; 55(9): 669 - 675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Gulley Molecular Diagnosis of Epstein-Barr Virus-Related Diseases J. Mol. Diagn., February 1, 2001; 3(1): 1 - 10. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Winberg, L. Matskova, F. Chen, P. Plant, D. Rotin, G. Gish, R. Ingham, I. Ernberg, and T. Pawson Latent Membrane Protein 2A of Epstein-Barr Virus Binds WW Domain E3 Protein-Ubiquitin Ligases That Ubiquitinate B-Cell Tyrosine Kinases Mol. Cell. Biol., November 15, 2000; 20(22): 8526 - 8535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K Takada Epstein-Barr virus and gastric carcinoma Mol. Pathol., October 1, 2000; 53(5): 255 - 261. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
