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[Cancer Research 60, 2770-2774, June 1, 2000]
© 2000 American Association for Cancer Research

Advances in Brief

Telomere Erosion Varies during in Vitro Aging of Normal Human Fibroblasts from Young and Adult Donors1

Reynel Figueroa2, Heike Lindenmaier, Manfred Hergenhahn, Kirsten Vang Nielsen and Petra Boukamp3

Divisions of Carcinogenesis and Differentiation [R. F., H. L., P. B.] and Genetic Alterations in Carcinogenesis [M. H.], German Cancer Research Center, D-69120 Heidelberg, Germany, and DAKO A/S, DK-2600 Glostrup, Denmark [K. V. N.]

The life span of normal fibroblasts in vitro (Hayflick limit) depends on donor age, and telomere shortening has been proposed as a potential mechanism. By quantitative fluorescence in situ hybridization and Southern blot analysis, we show progressive telomere loss to about 5 kb mean telomere restriction fragment length in fibroblasts from two adult donors within 40 population doublings, whereas in fibroblasts from two infant donors, telomere erosion is reduced, leaving a mean telomere restriction fragment length of ~7 kb at senescence (after ~60 population doublings). Aging of fibroblasts from both infant and adult donors was not accompanied by chromosomal abnormalities but was correlated with increased telomere repeat-binding factor 2 expression at both the protein and transcriptional level.




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Copyright © 2000 by the American Association for Cancer Research.