Cancer Research Meeting Calendar Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Richard, S. M.
Right arrow Articles by Bianchi, N. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Richard, S. M.
Right arrow Articles by Bianchi, N. O.
[Cancer Research 60, 4231-4237, August 1, 2000]
© 2000 American Association for Cancer Research

Molecular Biology and Genetics

Nuclear and Mitochondrial Genome Instability in Human Breast Cancer1

Silvina M. Richard, Graciela Bailliet, Gerardo L. Páez, Martha S. Bianchi, Päivi Peltomäki and Néstor O. Bianchi2

Instituto Multidisciplinario de Biología Celular, La Plata, Argentina [S. M. R., G. B., G. L. P., M. S. B., N. O. B.], and Division of Human Cancer Genetics, Ohio State University, Columbus, Ohio [P. P.]

We analyzed 40 pairs of breast normal/cancer tissues for the presence of mitochondrial (mt) genome instability and nuclear MSI in tumor cells. As mt, markers we used a (CA)n mt microsatellite (MS) starting at the 514-bp position of the D loop region and 4 informative MnlI sites located between the 16,108- and 16,420-bp positions of the D loop region. Nuclear microsatallite instability (MSI) was tested with 8 (CA)n MS, syntenic for the 13q chromosome arm. Moreover, we tested the spontaneous frequency of mtMSI and mt-MnlI mutations in 459 mother/descendant events. Mutations of mt-MnlI sites were found in 19 of 40 (47.5%) breast tumors, representing a 216-fold increase over the spontaneous rate in the female germline. Instability of the mtMS occurred in 17 of 40 (42.5%) breast cancers, which implies a 16-fold increase over the rate of spontaneous mutations. Nuclear MSI was found in 20 of 40 (50%) cases. In 15 of these cases the MSI was restricted to one locus, whereas in 5 instances the change of alleles was detected in 2 or 3 loci. Analysis of the correlation between mt and nuclear mutations showed no significant associations, suggesting that different systems are responsible for mt and nuclear genome instability in tumor cells. We propose that the two main mechanisms producing mtRFLP and mtMSI are damage by free radicals and error repair by the polymerase {gamma}, the first mechanism being a major cause of MnlI mutations and a secondary cause of mtMSI.




This article has been cited by other articles:


Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
C. Ye, Y.-T. Gao, W. Wen, J. P. Breyer, X. O. Shu, J. R. Smith, W. Zheng, and Q. Cai
Association of Mitochondrial DNA Displacement Loop (CA)n Dinucleotide Repeat Polymorphism with Breast Cancer Risk and Survival among Chinese Women
Cancer Epidemiol. Biomarkers Prev., August 1, 2008; 17(8): 2117 - 2122.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
W.-W. Jiang, E. Rosenbaum, E. Mambo, M. Zahurak, B. Masayesva, A. L. Carvalho, S. Zhou, W. H. Westra, A. J. Alberg, D. Sidransky, et al.
Decreased Mitochondrial DNA Content in Posttreatment Salivary Rinses from Head and Neck Cancer Patients
Clin. Cancer Res., March 1, 2006; 12(5): 1564 - 1569.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
J. P. Jakupciak, W. Wang, M. E. Markowitz, D. Ally, M. Coble, S. Srivastava, A. Maitra, P. E. Barker, D. Sidransky, and C. D. O'Connell
Mitochondrial DNA as a Cancer Biomarker
J. Mol. Diagn., May 1, 2005; 7(2): 258 - 267.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
W.-W. Jiang, B. Masayesva, M. Zahurak, A. L. Carvalho, E. Rosenbaum, E. Mambo, S. Zhou, K. Minhas, N. Benoit, W. H. Westra, et al.
Increased Mitochondrial DNA Content in Saliva Associated with Head and Neck Cancer
Clin. Cancer Res., April 1, 2005; 11(7): 2486 - 2491.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
W. Zhu, W. Qin, P. Bradley, A. Wessel, C. L. Puckett, and E. R. Sauter
Mitochondrial DNA mutations in breast cancer tissue and in matched nipple aspirate fluid
Carcinogenesis, January 1, 2005; 26(1): 145 - 152.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
A. Maitra, Y. Cohen, S. E.D. Gillespie, E. Mambo, N. Fukushima, M. O. Hoque, N. Shah, M. Goggins, J. Califano, D. Sidransky, et al.
The Human MitoChip: A High-Throughput Sequencing Microarray for Mitochondrial Mutation Detection
Genome Res., May 1, 2004; 14(5): 812 - 819.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
L-J C Wong, D-J Tan, R-K Bai, K-T Yeh, and J Chang
Molecular alterations in mitochondrial DNA of hepatocellular carcinomas: is there a correlation with clinicopathological profile?
J. Med. Genet., May 1, 2004; 41(5): e65 - e65.
[Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Tang, S. Baez, M. Pruyas, A. Diaz, A. Calvo, E. Riquelme, and I. I. Wistuba
Mitochondrial DNA Mutation at the D310 (Displacement Loop) Mononucleotide Sequence in the Pathogenesis of Gallbladder Carcinoma
Clin. Cancer Res., February 1, 2004; 10(3): 1041 - 1046.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Suzuki, S. Toyooka, K. Miyajima, T. Iizasa, T. Fujisawa, N. B. Bekele, and A. F. Gazdar
Alterations in the Mitochondrial Displacement Loop in Lung Cancers
Clin. Cancer Res., November 15, 2003; 9(15): 5636 - 5641.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
V. Maximo, P. Soares, J. Lima, J. Cameselle-Teijeiro, and M. Sobrinho-Simoes
Mitochondrial DNA Somatic Mutations (Point Mutations and Large Deletions) and Mitochondrial DNA Variants in Human Thyroid Pathology : A Study with Emphasis on Hurthle Cell Tumors
Am. J. Pathol., May 1, 2002; 160(5): 1857 - 1865.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Nekhaeva, N. D. Bodyak, Y. Kraytsberg, S. B. McGrath, N. J. Van Orsouw, A. Pluzhnikov, J. Y. Wei, J. Vijg, and K. Khrapko
Clonally expanded mtDNA point mutations are abundant in individual cells of human tissues
PNAS, April 16, 2002; 99(8): 5521 - 5526.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D.-J. Tan, R.-K. Bai, and L.-J. C. Wong
Comprehensive Scanning of Somatic Mitochondrial DNA Mutations in Breast Cancer
Cancer Res., February 1, 2002; 62(4): 972 - 976.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. Parrella, Y. Xiao, M. Fliss, M. Sanchez-Cespedes, P. Mazzarelli, M. Rinaldi, T. Nicol, E. Gabrielson, C. Cuomo, D. Cohen, et al.
Detection of Mitochondrial DNA Mutations in Primary Breast Cancer and Fine-Needle Aspirates
Cancer Res., October 1, 2001; 61(20): 7623 - 7626.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Nekhaeva, N. D. Bodyak, Y. Kraytsberg, S. B. McGrath, N. J. Van Orsouw, A. Pluzhnikov, J. Y. Wei, J. Vijg, and K. Khrapko
Clonally expanded mtDNA point mutations are abundant in individual cells of human tissues
PNAS, April 16, 2002; 99(8): 5521 - 5526.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2000 by the American Association for Cancer Research.