This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kang, S. Articles by Kennedy, G. C. Search for Related Content PubMed PubMed Citation Articles by Kang, S. Articles by Kennedy, G. C.

PCD1, a Novel Gene Containing PDZ and LIM Domains, Is Overexpressed in Several Human Cancers

Chiron Corp., Emeryville, California 94608

In an effort to discover novel genes differentially expressed in human pancreatic cancer, we have identified a gene named PCD1 (pancreatic cancer derived) that is up-regulated in pancreatic dysplasia and cancer relative to normal pancreatic ductal epithelium. We cloned the full length (4572 bp) of this gene, which encodes a novel protein of 1064 amino acids containing a PDZ domain and a LIM domain. An alternatively spliced form with a deletion of 30 bp in the coding region was also found. In situ hybridization results showed that PCD1 is highly expressed in a significant percentage of colon, breast, liver, lung, pancreas, stomach, and prostate tumor tissues but is expressed in very few normal tissues. Northern blot hybridization confirmed the overexpression of PCD1 in colon and breast tumor tissues and also showed strong expression of PCD1 in the heart as well as in HeLa cells. Real-time quantitative reverse transcription-PCR verified the overexpression of PCD1 in primary colon tumors or in liver metastases relative to normal colon tissues in five of eight patients. The PCD1 gene maps to human chromosome 13q21.33. Because of its high levels of expression in neoplastic tissues and the presence of both PDZ and LIM domains, we suggest that PCD1 may play an important role in cytoskeletal reorganization during carcinogenesis.

This article has been cited by other articles:

				J. Hollunder, M. Friedel, A. Beyer, C. T. Workman, and T. Wilhelm DASS: efficient discovery and p-value calculation of substructures in unordered data Bioinformatics, January 1, 2007; 23(1): 77 - 83. [Abstract] [Full Text] [PDF]

				B. Ryu, J. Jones, N. J. Blades, G. Parmigiani, M. A. Hollingsworth, R. H. Hruban, and S. E. Kern Relationships and Differentially Expressed Genes among Pancreatic Cancers Examined by Large-scale Serial Analysis of Gene Expression Cancer Res., February 1, 2002; 62(3): 819 - 826. [Abstract] [Full Text] [PDF]

				H. Chaib, M. A. Rubin, N. R. Mucci, L. Li, J. M. G. Taylor, M. L. Day, J. S. Rhim, and J. A. Macoska Activated in Prostate Cancer: A PDZ Domain-containing Protein Highly Expressed in Human Primary Prostate Tumors Cancer Res., March 1, 2001; 61(6): 2390 - 2394. [Abstract] [Full Text]