Cancer Research Infection and Cancer: Biology, Therapeutics, and Prevention
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cinti, C.
Right arrow Articles by Giordano, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cinti, C.
Right arrow Articles by Giordano, A.
[Cancer Research 60, 383-389, January 15, 2000]
© 2000 American Association for Cancer Research

Molecular Biology and Genetics

Genetic Alterations Disrupting the Nuclear Localization of the Retinoblastoma-related Gene RB2/p130 in Human Tumor Cell Lines and Primary Tumors1

Caterina Cinti, Pier Paolo Claudio, Candace M. Howard, Luca Maria Neri, Yan Fu, Lorenzo Leoncini, Gian Marco Tosi, Nadir Mario Maraldi and Antonio Giordano2

Istituto di Citomorfologia Normale e Patologica, CNR, 40136 Bologna, Italy [C. C., L. M. N., N. M. M.]; Departments of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 [P. P. C., C. M. H., Y. F., A. G.]; Dipartimento di Scienze Odontostomatologiche e Maxillo-Facciali, Università di Napoli "Federico II," 80100 Naples, Italy [P. P. C.]; Istituto di Anatomia Umana Normale, Università di Ferrara, 44100 Ferrara, Italy [L. M. N.]; Laboratorio di Biologia Cellulare e Microscopia Elettronica, Istituti di Ricerca "Codivilla Putti," IOR, 40100 Bologna, Italy [N. M. M.]; and Istituto di Anatomia e Istologia Patologica [L. L.] and Dipartimento di Scienze Oftalmologiche e Neurochirurgiche [G. M. T.], Università di Siena, 53100 Siena, Italy

The prototypic tumor suppressor gene, the retinoblastoma gene (RB/p105), is mutated in a variety of human tumors. However, to date, mutational data on retinoblastoma family members p107 and RB2/p130 in tumors is lacking. We studied the expression of pRb2/p130 by immunocytochemistry and Western blot analysis in a panel of human osteosarcoma and lymphoid cell lines. Only the lymphoid cell lines showed an abnormal cytoplasmic localization of pRb2/p130, suggesting possible alterations within the region of nuclear localization signaling. We screened these cell lines for genetic alterations of the RB2/p130 gene in the region of the putative bipartite nuclear localization signal (NLS). This region is highly homologous with that of the RB/p105 gene. In addition, we screened four primary Burkitt’s lymphomas for genetic alterations in the RB2/p130 gene. Naturally occurring mutations, which disrupt the putative bipartite NLS, were found in lymphoma cell lines and primary tumors, but not in the osteosarcoma cell lines, where normal nuclear localization of the protein was detectable. Site-directed mutagenesis and transfection assay using NLS mutants displayed markedly reduced biological activity as measured by flow cytometric analysis. This study clearly describes RB2/p130as an important target for mutations and subsequent inactivation in lymphoma pathogenesis, thus validating that RB2/p130 is a classical tumor suppressor gene.




This article has been cited by other articles:


Home page
 Postgrad. Med. J.Home page
G Brady, G J MacArthur, and P J Farrell
Epstein-Barr virus and Burkitt lymphoma
Postgrad. Med. J., July 1, 2008; 84(993): 372 - 377.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. L. Nguyen, A. N. Loveland, and W. Gibson
Nuclear Localization Sequences in Cytomegalovirus Capsid Assembly Proteins (UL80 Proteins) Are Required for Virus Production: Inactivating NLS1, NLS2, or Both Affects Replication to Strikingly Different Extents
J. Virol., June 1, 2008; 82(11): 5381 - 5389.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. J. Genovese, N. S. Banerjee, T. R. Broker, and L. T. Chow
Casein Kinase II Motif-Dependent Phosphorylation of Human Papillomavirus E7 Protein Promotes p130 Degradation and S-Phase Induction in Differentiated Human Keratinocytes
J. Virol., May 15, 2008; 82(10): 4862 - 4873.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. Rossi, V. Caracciolo, G. Russo, K. Reiss, and A. Giordano
Medulloblastoma: From Molecular Pathology to Therapy
Clin. Cancer Res., February 15, 2008; 14(4): 971 - 976.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
G Brady, G J MacArthur, and P J Farrell
Epstein Barr virus and Burkitt lymphoma
J. Clin. Pathol., December 1, 2007; 60(12): 1397 - 1402.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. De Falco, E. Leucci, D. Lenze, P. P. Piccaluga, P. P. Claudio, A. Onnis, G. Cerino, J. Nyagol, W. Mwanda, C. Bellan, et al.
Gene-expression analysis identifies novel RBL2/p130 target genes in endemic Burkitt lymphoma cell lines and primary tumors
Blood, August 15, 2007; 110(4): 1301 - 1307.
[Abstract] [Full Text] [PDF]

Home page
 ASH Education BookHome page
G. L. Kelly and A. B. Rickinson
Burkitt Lymphoma: Revisiting the Pathogenesis of a Virus-Associated Malignancy
Hematology, January 1, 2007; 2007(1): 277 - 284.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
G. Russo, A. Zamparelli, C. M. Howard, C. Minimo, C. Bellan, G. Carillo, L. Califano, L. Leoncini, A. Giordano, and P. P. Claudio
Expression of Cell Cycle-Regulated Proteins pRB2/p130, p107, E2F4, p27, and pCNA in Salivary Gland Tumors: Prognostic and Diagnostic Implications
Clin. Cancer Res., May 1, 2005; 11(9): 3265 - 3273.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
H. Huynh
Overexpression of tumour suppressor retinoblastoma 2 protein (pRb2/p130) in hepatocellular carcinoma
Carcinogenesis, August 1, 2004; 25(8): 1485 - 1494.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
C Bellan, S Lazzi, G De Falco, A Nyongo, A Giordano, and L Leoncini
Burkitt's lymphoma: new insights into molecular pathogenesis
J. Clin. Pathol., March 1, 2003; 56(3): 188 - 192.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
C. Bellan, G. De Falco, G. M. Tosi, S. Lazzi, F. Ferrari, G. Morbini, S. Bartolomei, P. Toti, P. Mangiavacchi, G. Cevenini, et al.
Missing Expression of pRb2/p130 in Human Retinoblastomas Is Associated with Reduced Apoptosis and Lesser Differentiation
Invest. Ophthalmol. Vis. Sci., December 1, 2002; 43(12): 3602 - 3608.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Caputi, A. M. Groeger, V. Esposito, A. De Luca, V. Masciullo, A. Mancini, F. Baldi, E. Wolner, and A. Giordano
Loss of pRb2/p130 Expression Is Associated with Unfavorable Clinical Outcome in Lung Cancer
Clin. Cancer Res., December 1, 2002; 8(12): 3850 - 3856.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
L Leoncini, S Lazzi, C Bellan, and P Tosi
Cell kinetics and cell cycle regulation in lymphomas
J. Clin. Pathol., September 1, 2002; 55(9): 648 - 655.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pathol.Home page
A J Alvi, R Hogg, J S Rader, M J Kuo, E R Maher, and F Latif
Mutation screening analysis of the retinoblastoma related gene RB2/p130 in sporadic ovarian cancer and head and neck squamous cell cancer
Mol. Pathol., June 1, 2002; 55(3): 153 - 155.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
P. P. Claudio, A. Zamparelli, F. U. Garcia, L. Claudio, G. Ammirati, A. Farina, A. Bovicelli, G. Russo, G. G. Giordano, D. E. McGinnis, et al.
Expression of Cell-Cycle-regulated Proteins pRb2/p130, p107, p27kip1, p53, mdm-2, and Ki-67 (MIB-1) in Prostatic Gland Adenocarcinoma
Clin. Cancer Res., June 1, 2002; 8(6): 1808 - 1815.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Chestukhin, L. Litovchick, K. Rudich, and J. A. DeCaprio
Nucleocytoplasmic Shuttling of p130/RBL2: Novel Regulatory Mechanism
Mol. Cell. Biol., January 15, 2002; 22(2): 453 - 468.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. S. Dimitrova and R. Berezney
The spatio-temporal organization of DNA replication sites is identical in primary, immortalized and transformed mammalian cells
J. Cell Sci., January 11, 2002; 115(21): 4037 - 4051.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. G. Paggi and A. Giordano
Who Is the Boss in the Retinoblastoma Family? The Point of View of Rb2/p130, the Little Brother
Cancer Res., June 1, 2001; 61(12): 4651 - 4654.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. M. Howard, P. P. Claudio, Antonio De Luca, P. Stiegler, F. P. Jori, N. M. Safdar, M. Caputi, K. Khalili, and A. Giordano
Inducible pRb2/p130 Expression and Growth-suppressive Mechanisms: Evidence of a pRb2/p130, p27Kip1, and Cyclin E Negative Feedback Regulatory Loop
Cancer Res., May 1, 2000; 60(10): 2737 - 2744.
[Abstract] [Full Text]

Home page
 Clin. Cancer Res.Home page
P. P. Claudio, M. Caputi, and A. Giordano
The RB2/p130 Gene: The Latest Weapon in the War against Lung Cancer?
Clin. Cancer Res., March 1, 2000; 6(3): 754 - 764.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
C. Cinti, L. Leoncini, A. Nyongo, F. Ferrari, S. Lazzi, C. Bellan, R. Vatti, A. Zamparelli, G. Cevenini, G. M. T osi, et al.
Genetic Alterations of the Retinoblastoma-Related Gene RB2/p130 Identify Different Pathogenetic Mechanisms in and among Burkitt’s Lymphoma Subtypes
Am. J. Pathol., March 1, 2000; 156(3): 751 - 760.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2000 by the American Association for Cancer Research.