| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Biochemistry and Biophysics |
Treatment Elevates Caspase-8 Expression and Sensitizes Human Breast Tumor Cells to a Death Receptor-induced Mitochondria-operated Apoptotic Program1
Instituto de Parasitología y Biomedicina Consejo Superior de Investigaciones Centificas, 18001 Granada, Spain
In this report, we have assessed the role of IFN-
as a sensitizing
agent in apoptosis mediated by activation of death receptor CD95 in
breast tumor cells. Treatment of the tumor cell lines MCF-7 and
MDA-MB231 with IFN- significantly facilitated apoptosis induced by
CD95 receptor ligation at the plasma membrane, independently of
p53 status. In contrast, IFN- treatment did not enhance the
apoptotic effect of the DNA-damaging drug, doxorubicin. Analysis of
apoptosis regulators indicated that caspase-8 mRNA and protein levels
were up-regulated in both of the cell lines after treatment with
IFN-. Furthermore, IFN- sensitized MCF-7 and MDA-MB231 cells to
CD95-mediated activation of caspase-8, induction of cytochrome
c release from mitochondria, and processing of
caspase-9. Release of cytochrome c, caspases activation,
and apoptosis were prevented in MCF-7 cells overexpressing Bcl-2.
Altogether these results indicate that IFN-, maybe through the
elevation of caspase-8 levels, sensitizes human breast tumor cells to a
death receptor-mediated, mitochondria-operated pathway of apoptosis.
This article has been cited by other articles:
|
|
 |
|
  T. Yoshimoto, N. Morishima, I. Mizoguchi, M. Shimizu, H. Nagai, S. Oniki, M. Oka, C. Nishigori, and J. Mizuguchi Antiproliferative Activity of IL-27 on Melanoma J. Immunol., May 15, 2008; 180(10): 6527 - 6535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lissat, T. Vraetz, M. Tsokos, R. Klein, M. Braun, N. Koutelia, P. Fisch, M. E. Romero, L. Long, P. Noellke, et al. Interferon-{gamma} Sensitizes Resistant Ewing's Sarcoma Cells to Tumor Necrosis Factor Apoptosis-Inducing Ligand-Induced Apoptosis by Up-Regulation of Caspase-8 Without Altering Chemosensitivity Am. J. Pathol., June 1, 2007; 170(6): 1917 - 1930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M.A. Rogers, M. Thomas, L. Galligan, T. R. Wilson, W. L. Allen, H. Sakai, P. G. Johnston, and D. B. Longley Cellular FLICE-inhibitory protein regulates chemotherapy-induced apoptosis in breast cancer cells Mol. Cancer Ther., May 1, 2007; 6(5): 1544 - 1551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. SCOLA, M. VAGLICA, A. CRIVELLO, L. PALMERI, G. I. FORTE, M. C. MACALUSO, A. GIACALONE, L. D. NOTO, A. BONGIOVANNI, C. RAIMONDI, et al. Cytokine Gene Polymorphisms and Breast Cancer Susceptibility Ann. N.Y. Acad. Sci., November 1, 2006; 1089(1): 104 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wu, X. Xiao, P. Zhao, G. Xue, Y. Zhu, X. Zhu, L. Zheng, Y. Zeng, and W. Huang Minicircle-IFN{gamma} Induces Antiproliferative and Antitumoral Effects in Human Nasopharyngeal Carcinoma Clin. Cancer Res., August 1, 2006; 12(15): 4702 - 4713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tesfaigzi Roles of Apoptosis in Airway Epithelia Am. J. Respir. Cell Mol. Biol., May 1, 2006; 34(5): 537 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Bausero, R. Gastpar, G. Multhoff, and A. Asea Alternative Mechanism by which IFN-{gamma} Enhances Tumor Recognition: Active Release of Heat Shock Protein 72 J. Immunol., September 1, 2005; 175(5): 2900 - 2912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. B. Bouker, T. C. Skaar, R. B. Riggins, D. S. Harburger, D. R. Fernandez, A. Zwart, A. Wang, and R. Clarke Interferon regulatory factor-1 (IRF-1) exhibits tumor suppressor activities in breast cancer associated with caspase activation and induction of apoptosis Carcinogenesis, September 1, 2005; 26(9): 1527 - 1535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Siegmund, A. Wicovsky, I. Schmitz, K. Schulze-Osthoff, S. Kreuz, M. Leverkus, O. Dittrich-Breiholz, M. Kracht, and H. Wajant Death Receptor-Induced Signaling Pathways Are Differentially Regulated by Gamma Interferon Upstream of Caspase 8 Processing Mol. Cell. Biol., August 1, 2005; 25(15): 6363 - 6379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pignatelli, J. Sanchez-Rodriguez, A. Santos, and A. Perez-Castillo 15-Deoxy-{Delta}-12,14-prostaglandin J2 induces programmed cell death of breast cancer cells by a pleiotropic mechanism Carcinogenesis, January 1, 2005; 26(1): 81 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Mrass, M. Rendl, M. Mildner, F. Gruber, B. Lengauer, C. Ballaun, L. Eckhart, and E. Tschachler Retinoic Acid Increases the Expression of p53 and Proapoptotic Caspases and Sensitizes Keratinocytes to Apoptosis: A Possible Explanation for Tumor Preventive Action of Retinoids Cancer Res., September 15, 2004; 64(18): 6542 - 6548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. Bouker, T. C. Skaar, D. R. Fernandez, K. A. O'Brien, R. B. Riggins, D. Cao, and R. Clarke Interferon Regulatory Factor-1 Mediates the Proapoptotic but Not Cell Cycle Arrest Effects of the Steroidal Antiestrogen ICI 182,780 (Faslodex, Fulvestrant) Cancer Res., June 1, 2004; 64(11): 4030 - 4039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ruiz-Ruiz, C. Ruiz de Almodovar, A. Rodriguez, G. Ortiz-Ferron, J. M. Redondo, and A. Lopez-Rivas The Up-regulation of Human Caspase-8 by Interferon-{gamma} in Breast Tumor Cells Requires the Induction and Action of the Transcription Factor Interferon Regulatory Factor-1 J. Biol. Chem., May 7, 2004; 279(19): 19712 - 19720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Burchert, D. Cai, L. C. Hofbauer, M. K. R. Samuelsson, E. P. Slater, J. Duyster, M. Ritter, A. Hochhaus, R. Muller, M. Eilers, et al. Interferon consensus sequence binding protein (ICSBP; IRF-8) antagonizes BCR/ABL and down-regulates bcl-2 Blood, May 1, 2004; 103(9): 3480 - 3489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Porras, S. Zuluaga, E. Black, A. Valladares, A. M. Alvarez, C. Ambrosino, M. Benito, and A. R. Nebreda p38{alpha} Mitogen-activated Protein Kinase Sensitizes Cells to Apoptosis Induced by Different Stimuli Mol. Biol. Cell, February 1, 2004; 15(2): 922 - 933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hoshiya, V. Gupta, H. Kawakubo, E. Brachtel, J. L. Carey, L. Sasur, A. Scott, P. K. Donahoe, and S. Maheswaran Mullerian Inhibiting Substance Promotes Interferon {gamma}-induced Gene Expression and Apoptosis in Breast Cancer Cells J. Biol. Chem., December 19, 2003; 278(51): 51703 - 51712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Bai and J. L. Merchant Transcription factor ZBP-89 is required for STAT1 constitutive expression Nucleic Acids Res., December 15, 2003; 31(24): 7264 - 7270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pingoud-Meier, D. Lang, A. J. Janss, L. B. Rorke, P. C. Phillips, T. Shalaby, and M. A. Grotzer Loss of Caspase-8 Protein Expression Correlates with Unfavorable Survival Outcome in Childhood Medulloblastoma Clin. Cancer Res., December 15, 2003; 9(17): 6401 - 6409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Geller, I. Petak, K. S. Szucs, K. Nagy, D. M. Tillman, and J. A. Houghton Interferon-{gamma}-Induced Sensitization of Colon Carcinomas to ZD9331 Targets Caspases, Downstream of Fas, Independent of Mitochondrial Signaling and the Inhibitor of Apoptosis Survivin Clin. Cancer Res., December 15, 2003; 9(17): 6504 - 6515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhou, Y. Zhang, J. A. Ardans, and L. M. Wahl Interferon-{gamma} Differentially Regulates Monocyte Matrix Metalloproteinase-1 and -9 through Tumor Necrosis Factor-{alpha} and Caspase 8 J. Biol. Chem., November 14, 2003; 278(46): 45406 - 45413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Simstein, M. Burow, A. Parker, C. Weldon, and B. Beckman Apoptosis, Chemoresistance, and Breast Cancer: Insights From the MCF-7 Cell Model System Experimental Biology and Medicine, October 1, 2003; 228(9): 995 - 1003. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Suarez, L. Gonzalez, A. Cuadrado, M. Berciano, M. Lafarga, and A. Munoz Kahalalide F, a new marine-derived compound, induces oncosis in human prostate and breast cancer cells Mol. Cancer Ther., September 1, 2003; 2(9): 863 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ruiz-Ruiz, G. Robledo, E. Cano, J. M. Redondo, and A. Lopez-Rivas Characterization of p53-mediated Up-regulation of CD95 Gene Expression upon Genotoxic Treatment in Human Breast Tumor Cells J. Biol. Chem., August 22, 2003; 278(34): 31667 - 31675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kalai, M. Lamkanfi, G. Denecker, M. Boogmans, S. Lippens, A. Meeus, W. Declercq, and P. Vandenabeele Regulation of the expression and processing of caspase-12 J. Cell Biol., August 4, 2003; 162(3): 457 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Loher, K. Schmall, P. Freytag, N. Landauer, R. Hallwachs, C. Bauer, B. Siegmund, F. Rieder, H.-A. Lehr, M. Dauer, et al. The Specific Type-4 Phosphodiesterase Inhibitor Mesopram Alleviates Experimental Colitis in Mice J. Pharmacol. Exp. Ther., May 1, 2003; 305(2): 549 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Munoz-Pinedo, C. Ruiz-Ruiz, C. Ruiz de Almodovar, C. Palacios, and A. Lopez-Rivas Inhibition of Glucose Metabolism Sensitizes Tumor Cells to Death Receptor-triggered Apoptosis through Enhancement of Death-inducing Signaling Complex Formation and Apical Procaspase-8 Processing J. Biol. Chem., April 4, 2003; 278(15): 12759 - 12768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Li, M. S. Kluger, L. A. Madge, L. Zheng, A. L. M. Bothwell, and J. S. Pober Interferon-{gamma} Augments CD95(APO-1/Fas) and Pro-Caspase-8 Expression and Sensitizes Human Vascular Endothelial Cells to CD95-Mediated Apoptosis Am. J. Pathol., October 1, 2002; 161(4): 1485 - 1495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. O-Quan Shi, M. J. Fischer, G. T. De Sanctis, M. R. Schuyler, and Y. Tesfaigzi IFN-{gamma}, But Not Fas, Mediates Reduction of Allergen-Induced Mucous Cell Metaplasia by Inducing Apoptosis J. Immunol., May 1, 2002; 168(9): 4764 - 4771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kumar-Sinha, S. Varambally, A. Sreekumar, and A. M. Chinnaiyan Molecular Cross-talk between the TRAIL and Interferon Signaling Pathways J. Biol. Chem., January 4, 2002; 277(1): 575 - 585. [Abstract] [Full Text]
|
|
|
|
|
|
N. Varela, C. Munoz-Pinedo, C. Ruiz-Ruiz, G. Robledo, M. Pedroso, and A. Lopez-Rivas Interferon-gamma Sensitizes Human Myeloid Leukemia Cells to Death Receptor-mediated Apoptosis by a Pleiotropic Mechanism J. Biol. Chem., May 18, 2001; 276(21): 17779 - 17787. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
