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[Cancer Research 60, 6230-6235, November 15, 2000]
© 2000 American Association for Cancer Research

Advances in Brief

Frequent Amplification of the Telomerase Reverse Transcriptase Gene in Human Tumors1

Anju Zhang2, Chengyun Zheng2, Charlotta Lindvall2, Mi Hou, Jessica Ekedahl, Rolf Lewensohn, Zhongqun Yan, Xiaoyan Yang, Marie Henriksson, Elisabeth Blennow, Magnus Nordenskjöld, Anders Zetterberg, Magnus Björkholm, Astrid Gruber and Dawei Xu3

Department of Oncology and Pathology [A. Zh., J. E., R. L., A. Ze.], Department of Medicine, Division of Hematology [C. Z., M. Ho., Z. Y., M. B., A. G., D. X., X. Y.], Department of Molecular Medicine [E. B., C. L., M. N.], and Microbiology & Tumor Biology Center [M. He.], Karolinska Hospital and Institutet, SE-171 76 Stockholm, Sweden

Activation of telomerase is a crucial step during cellular immortalization and malignant transformation of human cells and requires the induction of the catalytic component, human telomerase reverse transcriptase (hTERT), encoded by the hTERT gene. It is poorly understood how the hTERT gene is activated in human cancer cells. In the present study, we examined the hTERT gene copy number in human cancer cell lines and in primary tumor tissues. Amplification of the hTERT gene was observed in 8 of 26 (31%) tumor cell lines and 17 of 58 (30%) primary tumors examined (8 of 21 lung tumors, 3 of 10 cervical tumors, 5 of 19 breast carcinomas, and 1 of 8 neuroblastomas). In addition, 13 of 26 (50%) cell lines and 13 of 58 (22%) primary tumors displayed gain of hTERT gene copies with 3–4 copies/cell. The present findings imply that the hTERT locus may be a frequent target for amplification during tumorigenesis and that this genetic event probably contributes to the dysregulation of telomerase activity occurring in human tumors.




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