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[Cancer Research 60, 6581-6584, December 1, 2000]
© 2000 American Association for Cancer Research

Advances in Brief

DLC-1 Is Deleted in Primary Hepatocellular Carcinoma and Exerts Inhibitory Effects on the Proliferation of Hepatoma Cell Lines with Deleted DLC-11

Irene Oi-lin Ng2, Zheng-dong Liang, Liang Cao and Terence Kin-wah Lee

Departments of Pathology [I. O. N., Z-D. L., T. K. L.] and Microbiology [L. C.], University of Hong Kong, Queen Mary Hospital, Hong Kong, China

We investigated the expression and deletion of DLC-1 (frequently deleted in liver cancer gene), first reported in 1998 and having a high homology with rat p122RhoGAP in hepatocellular carcinoma (HCC). Six (20%) of 30 human HCC samples and 2 (40%) of 5 HCC cell lines were found to have no detectable DLC-1 expression by reverse transcription-PCR. Homozygous DLC-1 deletion was detected by Southern blotting in two of six HCC samples and in both HCC cell lines with no DLC-1 expression. Transfection of DLC-1 into 5 HCC cell lines (two with DLC-1 deletion and three with intact DLC-1) showed significant growth inhibition in these two HCC cell lines with deleted DLC-1 with both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays but not in three other HCC cell lines with intact DLC-1. Our findings suggest that DLC-1 may play an important role in hepatocarcinogenesis.




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