Cancer Research Grants Advances in Breast Cancer Research
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boczkowski, D.
Right arrow Articles by Gilboa, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boczkowski, D.
Right arrow Articles by Gilboa, E.
[Cancer Research 60, 1028-1034, February 15, 2000]
© 2000 American Association for Cancer Research

Immunology

Induction of Tumor Immunity and Cytotoxic T Lymphocyte Responses Using Dendritic Cells Transfected with Messenger RNA Amplified from Tumor Cells1

David Boczkowski, Smita K. Nair, Jong-Hee Nam, H. Kim Lyerly and Eli Gilboa2

Center for Genetic and Cellular Therapies, Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710

Unique patient-specific tumor antigens may constitute the dominant antigens in the antitumor immune response. Hence, vaccination with the patient’s own repertoire of tumor antigens may offer a superior strategy to elicit protective immunity. We have shown previously that dendritic cells transfected with mRNA isolated from tumor cells stimulate potent CTL responses and engender protective immunity in tumor-bearing mice. In the current study, we demonstrate that tumor mRNA, isolated from murine tumor cell lines or from primary human tumor cells microdissected from frozen tissue sections, can be amplified without loss of function. This study provides the foundations for an effective and broadly applicable treatment that does not require the characterization of the relevant antigenic profile in each patient and will not be limited by tumor tissue availability for antigen preparation.




This article has been cited by other articles:


Home page
 BloodHome page
S.-i. Fujii, A. Goto, and K. Shimizu
Antigen mRNA-transfected, allogeneic fibroblasts loaded with NKT-cell ligand confer antitumor immunity
Blood, April 30, 2009; 113(18): 4262 - 4272.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. Osada, C. Y. Woo, M. McKinney, X. Y. Yang, G. Lei, H. G. LaBreche, Z. C. Hartman, D. Niedzwiecki, N. Chao, A. Amalfitano, et al.
Induction of Wilms' Tumor Protein (WT1)-Specific Antitumor Immunity Using a Truncated WT1-Expressing Adenovirus Vaccine
Clin. Cancer Res., April 15, 2009; 15(8): 2789 - 2796.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. N. Franki, K. K. Steward, D. J. Betting, K. Kafi, R. E. Yamada, and J. M. Timmerman
Dendritic cells loaded with apoptotic antibody-coated tumor cells provide protective immunity against B-cell lymphoma in vivo
Blood, February 1, 2008; 111(3): 1504 - 1511.
[Abstract] [Full Text] [PDF]

Home page
 ASH ANNUAL MEETING ABSTRACTSHome page
X. Ni, H. Richmond, E. Shpall, and M. Duvic
Induction of Polyclonal T-Cell Responses Against Cutaneous T-Cell Lymphomas by Autologous Dendritic Cells Transfected with Amplified Tumor RNA.
Blood (ASH Annual Meeting Abstracts), November 16, 2006; 108(11): 5189 - 5189.
[Abstract] [PDF]

Home page
 Cancer Res.Home page
S.-C. Yang, R. K. Batra, S. Hillinger, K. L. Reckamp, R. M. Strieter, S. M. Dubinett, and S. Sharma
Intrapulmonary Administration of CCL21 Gene-Modified Dendritic Cells Reduces Tumor Burden in Spontaneous Murine Bronchoalveolar Cell Carcinoma.
Cancer Res., March 15, 2006; 66(6): 3205 - 3213.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Fassnacht, J. Lee, C. Milazzo, D. Boczkowski, Z. Su, S. Nair, and E. Gilboa
Induction of CD4+ and CD8+ T-Cell Responses to the Human Stromal Antigen, Fibroblast Activation Protein: Implication for Cancer Immunotherapy
Clin. Cancer Res., August 1, 2005; 11(15): 5566 - 5571.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. Dannull, S. Nair, Z. Su, D. Boczkowski, C. DeBeck, B. Yang, E. Gilboa, and J. Vieweg
Enhancing the immunostimulatory function of dendritic cells by transfection with mRNA encoding OX40 ligand
Blood, April 15, 2005; 105(8): 3206 - 3213.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
F. Ito, Q. Li, A. B. Shreiner, R. Okuyama, M. N. Jure-Kunkel, S. Teitz-Tennenbaum, and A. E. Chang
Anti-CD137 Monoclonal Antibody Administration Augments the Antitumor Efficacy of Dendritic Cell-Based Vaccines
Cancer Res., November 15, 2004; 64(22): 8411 - 8419.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. Xue, E. Stavropoulos, M. Yang, S. Ragno, M. Vordermeier, M. Chambers, G. Hewinson, D. B. Lowrie, M. J. Colston, and R. E. Tascon
RNA Encoding the MPT83 Antigen Induces Protective Immune Responses against Mycobacterium tuberculosis Infection
Infect. Immun., November 1, 2004; 72(11): 6324 - 6329.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. M. Cisco, Z. Abdel-Wahab, J. Dannull, S. Nair, D. S. Tyler, E. Gilboa, J. Vieweg, Y. Daaka, and S. K. Pruitt
Induction of Human Dendritic Cell Maturation Using Transfection with RNA Encoding a Dominant Positive Toll-Like Receptor 4
J. Immunol., June 1, 2004; 172(11): 7162 - 7168.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. Murakami, N. Tokunaga, T. Waku, S. Gomi, S. Kagawa, N. Tanaka, and T. Fujiwara
Antitumor Effect of Intratumoral Administration of Bone Marrow-Derived Dendritic Cells Transduced with Wild-Type p53 Gene
Clin. Cancer Res., June 1, 2004; 10(11): 3871 - 3880.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C. M. Coughlin, B. A. Vance, S. A. Grupp, and R. H. Vonderheide
RNA-transfected CD40-activated B cells induce functional T-cell responses against viral and tumor antigen targets: implications for pediatric immunotherapy
Blood, March 15, 2004; 103(6): 2046 - 2054.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. R. Muller, G. Tsakou, F. Grunebach, S. M. Schmidt, and P. Brossart
Induction of chronic lymphocytic leukemia (CLL)-specific CD4- and CD8-mediated T-cell responses using RNA-transfected dendritic cells
Blood, March 1, 2004; 103(5): 1763 - 1769.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. T. van den Broeke, E. Daschbach, E. K. Thomas, G. Andringa, and J. A. Berzofsky
Dendritic Cell-Induced Activation of Adaptive and Innate Antitumor Immunity
J. Immunol., December 1, 2003; 171(11): 5842 - 5852.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Nair, C. McLaughlin, A. Weizer, Z. Su, D. Boczkowski, J. Dannull, J. Vieweg, and E. Gilboa
Injection of Immature Dendritic Cells into Adjuvant-Treated Skin Obviates the Need for Ex Vivo Maturation
J. Immunol., December 1, 2003; 171(11): 6275 - 6282.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Zhao, D. Boczkowski, S. K. Nair, and E. Gilboa
Inhibition of invariant chain expression in dendritic cells presenting endogenous antigens stimulates CD4+ T-cell responses and tumor immunity
Blood, December 1, 2003; 102(12): 4137 - 4142.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Satoh, T. Saika, S. Ebara, N. Kusaka, T. L. Timme, G. Yang, J. Wang, V. Mouraviev, G. Cao, E. M. A. Fattah, et al.
Macrophages Transduced with an Adenoviral Vector Expressing Interleukin 12 Suppress Tumor Growth and Metastasis in a Preclinical Metastatic Prostate Cancer Model
Cancer Res., November 15, 2003; 63(22): 7853 - 7860.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Santulli-Marotto, S. K. Nair, C. Rusconi, B. Sullenger, and E. Gilboa
Multivalent RNA Aptamers That Inhibit CTLA-4 and Enhance Tumor Immunity
Cancer Res., November 1, 2003; 63(21): 7483 - 7489.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
D. W. Mullins, S. L. Sheasley, R. M. Ream, T. N.J. Bullock, Y.-X. Fu, and V. H. Engelhard
Route of Immunization with Peptide-pulsed Dendritic Cells Controls the Distribution of Memory and Effector T Cells in Lymphoid Tissues and Determines the Pattern of Regional Tumor Control
J. Exp. Med., October 6, 2003; 198(7): 1023 - 1034.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. R. Muller, F. Grunebach, A. Nencioni, and P. Brossart
Transfection of Dendritic Cells with RNA Induces CD4- and CD8-Mediated T Cell Immunity Against Breast Carcinomas and Reveals the Immunodominance of Presented T Cell Epitopes
J. Immunol., June 15, 2003; 170(12): 5892 - 5896.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
I. D. Davis, M. Jefford, P. Parente, and J. Cebon
Rational approaches to human cancer immunotherapy
J. Leukoc. Biol., January 1, 2003; 73(1): 3 - 29.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Z. Su, J. Vieweg, A. Z. Weizer, P. Dahm, D. Yancey, V. Turaga, J. Higgins, D. Boczkowski, E. Gilboa, and J. Dannull
Enhanced Induction of Telomerase-specific CD4+ T Cells Using Dendritic Cells Transfected with RNA Encoding a Chimeric Gene Product
Cancer Res., September 1, 2002; 62(17): 5041 - 5048.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Spisek, P. Chevallier, N. Morineau, N. Milpied, H. Avet-Loiseau, J.-L. Harousseau, K. Meflah, and M. Gregoire
Induction of Leukemia-specific Cytotoxic Response by Cross-Presentation of Late-Apoptotic Leukemic Blasts by Autologous Dendritic Cells of Nonleukemic Origin
Cancer Res., May 1, 2002; 62(10): 2861 - 2868.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Kawarada, R. Ganss, N. Garbi, T. Sacher, B. Arnold, and G. J. Hammerling
NK- and CD8+ T Cell-Mediated Eradication of Established Tumors by Peritumoral Injection of CpG-Containing Oligodeoxynucleotides
J. Immunol., November 1, 2001; 167(9): 5247 - 5253.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. W. Ju, Q. Tao, G. Lou, M. Bai, L. He, Y. Yang, and X. Cao
Interleukin 18 Transfection Enhances Antitumor Immunity Induced by Dendritic Cell-Tumor Cell Conjugates
Cancer Res., May 1, 2001; 61(9): 3735 - 3740.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
S. Paczesny, S. Beranger, J.-L. Salzmann, D. Klatzmann, and B. M. Colombo
Protection of Mice against Leukemia after Vaccination with Bone Marrow-derived Dendritic Cells Loaded with Apoptotic Leukemia Cells
Cancer Res., March 1, 2001; 61(6): 2386 - 2389.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
A. Heiser, M. A. Maurice, D. R. Yancey, N. Z. Wu, P. Dahm, S. K. Pruitt, D. Boczkowski, S. K. Nair, M. S. Ballo, E. Gilboa, et al.
Induction of Polyclonal Prostate Cancer-Specific CTL Using Dendritic Cells Transfected with Amplified Tumor RNA
J. Immunol., March 1, 2001; 166(5): 2953 - 2960.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
J. Baggers, G. Ratzinger, and J. W. Young
Dendritic Cells as Immunologic Adjuvants for the Treatment of Cancer
J. Clin. Oncol., December 1, 2000; 18(23): 3879 - 3882.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2000 by the American Association for Cancer Research.