This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yoon, G.-S. Articles by Lee, I. Search for Related Content PubMed PubMed Citation Articles by Yoon, G.-S. Articles by Lee, I.

Nuclear Matrix of Calreticulin in Hepatocellular Carcinoma¹

Departments of Pathology [G-S. Y., H. L., Y. J., E. Y., I. L.], Internal Medicine [H-B. M.], and Biochemistry [K. S.], University of Ulsan College of Medicine and Asan Institute for Life Sciences, Seoul, Korea

Nuclear matrix protein profiles of malignant cells vary from their normal counterparts. By two-dimensional gel electrophoresis, we analyzed nuclear matrix proteins in 11 hepatocellular carcinomas and compared them with corresponding non-neoplastic liver tissue. Although the compositions were mostly similar, several peptides were noted predominantly in the former. The most prominent one was an acidic protein of apparent M_r 62,000, which was identified to be calreticulin upon NH₂-terminal amino acid sequencing. By immunoblotting, calreticulin was confirmed to be present abundantly in the nuclear matrix fraction of carcinomas but not in that of the nonmalignant liver tissue. Interestingly, the total content of calreticulin was similar between them. By immunofluorescence microscopy, evident nuclear immunostaining was detected in carcinomas. Calreticulin was also found to be in the nuclear matrices of various carcinoma cell lines. We conclude that calreticulin is a component of the nuclear matrix. The formation and/or expansion of the calreticulin-nuclear matrix may be related to the activated cell growth.

This article has been cited by other articles:

				L. B. Nanney, C. D. Woodrell, M. R. Greives, N. L. Cardwell, A. C. Pollins, T. A. Bancroft, A. Chesser, M. Michalak, M. Rahman, J. W. Siebert, et al. Calreticulin Enhances Porcine Wound Repair by Diverse Biological Effects Am. J. Pathol., September 1, 2008; 173(3): 610 - 630. [Abstract] [Full Text] [PDF]

				Y. Hayashida, Y. Urata, E. Muroi, T. Kono, Y. Miyata, K. Nomata, H. Kanetake, T. Kondo, and Y. Ihara Calreticulin Represses E-cadherin Gene Expression in Madin-Darby Canine Kidney Cells via Slug J. Biol. Chem., October 27, 2006; 281(43): 32469 - 32484. [Abstract] [Full Text] [PDF]

				W. M. Hsu, F. J. Hsieh, Y. M. Jeng, M. L. Kuo, C. N. Chen, D. M. Lai, L. J. Hsieh, B. T. Wang, P. N. Tsao, H. Lee, et al. Calreticulin expression in neuroblastoma--a novel independent prognostic factor Ann. Onc., February 1, 2005; 16(2): 314 - 321. [Abstract] [Full Text] [PDF]

				C. G. Levine, D. Mitra, A. Sharma, C. L. Smith, and R. S. Hegde The Efficiency of Protein Compartmentalization into the Secretory Pathway Mol. Biol. Cell, January 1, 2005; 16(1): 279 - 291. [Abstract] [Full Text] [PDF]

				S. Kageyama, T. Isono, H. Iwaki, Y. Wakabayashi, Y. Okada, K. Kontani, K. Yoshimura, A. Terai, Y. Arai, and T. Yoshiki Identification by Proteomic Analysis of Calreticulin as a Marker for Bladder Cancer and Evaluation of the Diagnostic Accuracy of Its Detection in Urine Clin. Chem., May 1, 2004; 50(5): 857 - 866. [Abstract] [Full Text] [PDF]

				G. Brunagel, B. N. Vietmeier, A. J. Bauer, R. E. Schoen, and R. H. Getzenberg Identification of Nuclear Matrix Protein Alterations Associated with Human Colon Cancer Cancer Res., April 1, 2002; 62(8): 2437 - 2442. [Abstract] [Full Text] [PDF]

				F. Le Naour, F. Brichory, D. E. Misek, C. Brechot, S. M. Hanash, and L. Beretta A Distinct Repertoire of Autoantibodies in Hepatocellular Carcinoma Identified by Proteomic Analysis Mol. Cell. Proteomics, March 1, 2002; 1(3): 197 - 203. [Abstract] [Full Text] [PDF]