Cancer Research Infection and Cancer: Biology, Therapeutics, and Prevention Translational Medicine Conference in Israel
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paige, A. J. W.
Right arrow Articles by Watson, J. E. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paige, A. J. W.
Right arrow Articles by Watson, J. E. V.
[Cancer Research 60, 1690-1697, March 15, 2000]
© 2000 American Association for Cancer Research

Molecular Biology and Genetics

A 700-kb Physical Map of a Region of 16q23.2 Homozygously Deleted in Multiple Cancers and Spanning the Common Fragile Site FRA16D1

Adam J. W. Paige, Karen J. Taylor, Aengus Stewart, John G. Sgouros, Hani Gabra, Grant C. Sellar, John F. Smyth, David J. Porteous and J. E. Vivienne Watson2

Imperial Cancer Research Fund [A. J. W. P. , K. J. T., H. G., G. C. S., J. F. S., J. E. V. W.] and MRC Human Genetics Unit [D. J. P.], Western General Hospital, Edinburgh EH4 2XU, United Kingdom, and Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom [A. S., J. G. S.]

We have identified a >600-kb region at 16q23.2 that is homozygously deleted from malignant ovarian ascites using representational difference analysis. Overlapping homozygous deletions were also observed in the colon carcinoma cell line HCT116 and a xenograft established from the small cell lung cancer cell line WX330. This region coincides with that described previously by others as showing loss of heterozygosity in prostate and breast cancers (C. Li et al., Genes Chromosomes Cancer, 24: 175–182, 1999; A. Latil et al., Cancer Res., 57: 1058–1062, 1997; K. Driouch et al., Genes Chromosomes Cancer, 19: 185–191, 1997; A. Iida et al., Br. J. Cancer, 75: 264–267, 1997). In addition, the minimally deleted region spans the common fragile site FRA16D. We have constructed a 700-kb physical map encompassing the deleted region. By fluorescence in situ hybridization of aphidicolin-induced metaphase chromosomes, we have preliminary data to suggest that P1-derived bacterial artificial chromosome clones from the contig lie on both sides of FRA16D. This is confirmed by extensive fluorescence in situ hybridization analysis of the region reported in the accompanying article (M. Mangelsdorf et al., Cancer Res., 60:1683–1689, 2000) and is consistent with an involvement of this common fragile site in the loss of 16q23.2 material in various cancer types. The minimally deleted region of approximately 210 kb has been characterized using our own markers and public domain markers. Eleven distinct expressed sequences mapped to the region, providing a basis for identifying the predicted tumor suppressor gene in this region.




This article has been cited by other articles:


Home page
 Hum Mol GenetHome page
T. W. Glover, M. F. Arlt, A. M. Casper, and S. G. Durkin
Mechanisms of common fragile site instability
Hum. Mol. Genet., October 15, 2005; 14(suppl_2): R197 - R205.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Finnis, S. Dayan, L. Hobson, G. Chenevix-Trench, K. Friend, K. Ried, D. Venter, E. Woollatt, E. Baker, and R. I. Richards
Common chromosomal fragile site FRA16D mutation in cancer cells
Hum. Mol. Genet., May 15, 2005; 14(10): 1341 - 1349.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Musio, C. Montagna, T. Mariani, M. Tilenni, M. L. Focarelli, L. Brait, E. Indino, P. A. Benedetti, L. Chessa, A. Albertini, et al.
SMC1 involvement in fragile site expression
Hum. Mol. Genet., February 15, 2005; 14(4): 525 - 533.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. T. Barrett, A. Scheffer, A. Ben-Dor, N. Sampas, D. Lipson, R. Kincaid, P. Tsang, B. Curry, K. Baird, P. S. Meltzer, et al.
Comparative genomic hybridization using oligonucleotide microarrays and total genomic DNA
PNAS, December 21, 2004; 101(51): 17765 - 17770.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
R. I. Aqeilan, T. Kuroki, Y. Pekarsky, O. Albagha, F. Trapasso, R. Baffa, K. Huebner, P. Edmonds, and C. M. Croce
Loss of WWOX Expression in Gastric Carcinoma
Clin. Cancer Res., May 1, 2004; 10(9): 3053 - 3058.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. Kuroki, S. Yendamuri, F. Trapasso, A. Matsuyama, R. I. Aqeilan, H. Alder, S. Rattan, R. Cesari, M. L. Nolli, N. N. Williams, et al.
The Tumor Suppressor Gene WWOX at FRA16D Is Involved in Pancreatic Carcinogenesis
Clin. Cancer Res., April 1, 2004; 10(7): 2459 - 2465.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Watanabe, Y. Hippo, H. Taniguchi, H. Iwanari, M. Yashiro, K. Hirakawa, T. Kodama, and H. Aburatani
An Opposing View on WWOX Protein Function as a Tumor Suppressor
Cancer Res., December 15, 2003; 63(24): 8629 - 8633.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. A. Jackson, A. V. Trevino, M. C. Herzig, T. S. Herman, and J. M. Woynarowski
Matrix attachment region (MAR) properties and abnormal expansion of AT island minisatellites in FRA16B fragile sites in leukemic CEM cells
Nucleic Acids Res., November 1, 2003; 31(21): 6354 - 6364.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Corbin, M. E. Neilly, R. Espinosa III, E. M. Davis, T. W. McKeithan, and M. M. Le Beau
Identification of Unstable Sequences within the Common Fragile Site at 3p14.2: Implications for the Mechanism of Deletions within Fragile Histidine Triad Gene/Common Fragile Site at 3p14.2 in Tumors
Cancer Res., June 1, 2002; 62(12): 3477 - 3484.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Kuroki, F. Trapasso, T. Shiraishi, H. Alder, K. Mimori, M. Mori, and C. M. Croce
Genetic Alterations of the Tumor Suppressor Gene WWOX in Esophageal Squamous Cell Carcinoma
Cancer Res., April 1, 2002; 62(8): 2258 - 2260.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. K. Bednarek, C. L. Keck-Waggoner, R. L. Daniel, K. J. Laflin, P. L. Bergsagel, K. Kiguchi, A. J. Brenner, and C. M. Aldaz
WWOX, the FRA16D Gene, Behaves as a Suppressor of Tumor Growth
Cancer Res., November 1, 2001; 61(22): 8068 - 8073.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. J. W. Paige, K. J. Taylor, C. Taylor, S. G. Hillier, S. Farrington, D. Scott, D. J. Porteous, J. F. Smyth, H. Gabra, and J. E. V. Watson
WWOX: A candidate tumor suppressor gene involved in multiple tumor types
PNAS, September 25, 2001; 98(20): 11417 - 11422.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Shiraishi, T. Druck, K. Mimori, J. Flomenberg, L. Berk, H. Alder, W. Miller, K. Huebner, and C. M. Croce
Sequence conservation at human and mouse orthologous common fragile regions, FRA3B/FHIT and Fra14A2/Fhit
PNAS, April 18, 2001; (2001) 91095898.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
E. C. Thorland, S. L. Myers, D. H. Persing, G. Sarkar, R. M. McGovern, B. S. Gostout, and D. I. Smith
Human Papillomavirus Type 16 Integrations in Cervical Tumors Frequently Occur in Common Fragile Sites
Cancer Res., November 1, 2000; 60(21): 5916 - 5921.
[Abstract] [Full Text]

Home page
 Hum Mol GenetHome page
K. Ried, M. Finnis, L. Hobson, M. Mangelsdorf, S. Dayan, J. K. Nancarrow, E. Woollatt, G. Kremmidiotis, A. Gardner, D. Venter, et al.
Common chromosomal fragile site FRA16D sequence: identification of the FOR gene spanning FRA16D and homozygous deletions and translocation breakpoints in cancer cells
Hum. Mol. Genet., July 1, 2000; 9(11): 1651 - 1663.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. L. Paris, J. S. Witte, P. A. Kupelian, H. Levin, E. A. Klein, W. J. Catalona, and G. Casey
Identification and Fine Mapping of a Region Showing a High Frequency of Allelic Imbalance on Chromosome 16q23.2 That Corresponds to a Prostate Cancer Susceptibility Locus
Cancer Res., July 1, 2000; 60(13): 3645 - 3649.
[Abstract] [Full Text]

Home page
 Cancer Res.Home page
M. Mangelsdorf, K. Ried, E. Woollatt, S. Dayan, H. Eyre, M. Finnis, L. Hobson, J. Nancarrow, D. Venter, E. Baker, et al.
Chromosomal Fragile Site FRA16D and DNA Instability in Cancer
Cancer Res., March 1, 2000; 60(6): 1683 - 1689.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
N.-S. Chang, N. Pratt, J. Heath, L. Schultz, D. Sleve, G. B. Carey, and N. Zevotek
Hyaluronidase Induction of a WW Domain-containing Oxidoreductase That Enhances Tumor Necrosis Factor Cytotoxicity
J. Biol. Chem., January 26, 2001; 276(5): 3361 - 3370.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Shiraishi, T. Druck, K. Mimori, J. Flomenberg, L. Berk, H. Alder, W. Miller, K. Huebner, and C. M. Croce
Sequence conservation at human and mouse orthologous common fragile regions, FRA3B/FHIT and Fra14A2/Fhit
PNAS, May 8, 2001; 98(10): 5722 - 5727.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 2000 by the American Association for Cancer Research.