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Metabolites of 2'-Fluoro-2'-deoxy-D-glucose Detected by ¹⁹F Magnetic Resonance Spectroscopy in Vivo Predict Response of Murine RIF-1 Tumors to 5-Fluorouracil¹

Cancer Research Campaign Biomedical Magnetic Resonance Research Unit, Department of Biochemistry and Immunology, St. George’s Hospital Medical School, London SW17 0RE, United Kingdom [P. M. J. M., J. R. G.], and Cancer Research Campaign Clinical Magnetic Resonance Research Group [P. M. J. M., M. O. L.] and Cancer Research Campaign Centre for Cancer Therapeutics [P. M. J. M., I. R. J.], Institute of Cancer Research and Royal Marsden NHS Trust, Sutton, Surrey SM2, United Kingdom

There is a clinical need for early detection of tumor response to therapy. This study aimed to determine whether metabolites of fluorodeoxyglucose (FDG) detected in solid mouse tumors in situ by ¹⁹F magnetic resonance spectroscopy (¹⁹F MRS) correlated with response to 5-fluorouracil chemotherapy. After injection of FDG (1.4 mmol/kg i.p.), uptake and metabolism was monitored for 2 h in RIF-1 tumors. FDG was detectable immediately, and after 10 min, a second broad peak was detected 5–6 ppm upfield. ¹⁹F MRS analysis of cell and tumor extracts in vitro showed that the upfield peak (15% of the total detectable ¹⁹F signal) consisted of the epimer -fluorodeoxymannose (FDM) and various conjugates. Mice treated with 5-fluorouracil (130 mg/kg) received, 48 h later, a repeat dose of FDG. The change in the rate of FDM formation, but not the FDG or total ¹⁹F signal, correlated significantly with the response to 5-fluorouracil (P = 0.032), suggesting that ¹⁹F MRS of FDM metabolism in vivo may be a novel means of predicting tumor response.

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