This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murphy, G. A. Articles by McLennan, A. G. Search for Related Content PubMed PubMed Citation Articles by Murphy, G. A. Articles by McLennan, A. G.

The Fhit Tumor Suppressor Protein Regulates the Intracellular Concentration of Diadenosine Triphosphate but not Diadenosine Tetraphosphate¹

Cell Regulation and Signalling Group, School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, United Kingdom

ABSTRACT

To determine the role of the FHIT tumor suppressor gene product, a diadenosine 5',5'''-P¹,P³-triphosphate (Ap₃A) hydrolase, in the regulation of the concentration of Ap₃A and diadenosine 5',5'''-P¹,P⁴-tetraphosphate (Ap₄A) in vivo, the levels of the adenosine(5')triphospho(5')nucleoside (Ap₃N) and adenosine(5')tetraphospho(5')nucleoside (Ap₄N) families were measured by luminometry in a number of human cell lines and correlated with the expression of Fhit determined by immunoblotting. Fhit-positive cells had no Ap₃N or a very low level of Ap₃N, whereas most Fhit-negative cells had Ap₃N in the range 0.2–0.9 pmol/10⁶ cells. Ap₄N (mean value, 0.17 pmol/10⁶ cells) did not correlate with Fhit expression. The results suggest that Fhit efficiently metabolizes Ap₃A and Ap₃N but not Ap₄A or Ap₄N in vivo.

This article has been cited by other articles:

				Y.-N. Lee and E. Razin Nonconventional Involvement of LysRS in the Molecular Mechanism of USF2 Transcriptional Activity in Fc{varepsilon}RI-Activated Mast Cells Mol. Cell. Biol., October 15, 2005; 25(20): 8904 - 8912. [Abstract] [Full Text] [PDF]

				Y. Pekarsky, P. N. Garrison, A. Palamarchuk, N. Zanesi, R. I. Aqeilan, K. Huebner, L. D. Barnes, and C. M. Croce Fhit is a physiological target of the protein kinase Src PNAS, March 16, 2004; 101(11): 3775 - 3779. [Abstract] [Full Text] [PDF]

				T. M. Ismail, C. A. Hart, and A. G. McLennan Regulation of Dinucleoside Polyphosphate Pools by the YgdP and ApaH Hydrolases Is Essential for the Ability of Salmonella enterica serovar Typhimurium to Invade Cultured Mammalian Cells J. Biol. Chem., August 29, 2003; 278(35): 32602 - 32607. [Abstract] [Full Text] [PDF]

				N. Korsisaari, D. J. Rossi, K. Luukko, K. Huebner, M. Henkemeyer, and T. P. Makela The Histidine Triad Protein Hint Is Not Required for Murine Development or Cdk7 Function Mol. Cell. Biol., June 1, 2003; 23(11): 3929 - 3935. [Abstract] [Full Text] [PDF]

				J. I. Lee, J.-C. Soria, K. Hassan, D. Liu, X. Tang, A. El-Naggar, W. K. Hong, and L. Mao Loss of Fhit Expression Is a Predictor of Poor Outcome in Tongue Cancer Cancer Res., February 1, 2001; 61(3): 837 - 841. [Abstract] [Full Text]