This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Waghray, A. Articles by Chen, Y. Q. Search for Related Content PubMed PubMed Citation Articles by Waghray, A. Articles by Chen, Y. Q.

Identification of Differentially Expressed Genes by Serial Analysis of Gene Expression in Human Prostate Cancer¹

Department of Pathology [A. W., M. S., F. F., F. Y., J. V., Y. Q. C.] and Center for Molecular Medicine and Genetics [Y. Q. C.], Wayne State University, Detroit, Michigan 48201

Prostate cancer is the leading cause of cancer death in American males. To better understand the genetic bases of this disease, we have generated a comprehensive molecular profile of human prostate. The gene expression pattern in normal and prostate cancer tissues was analyzed by serial analysis of gene expression (SAGE). A total of 133,217 transcripts were analyzed, and 35,185 distinct SAGE tags were identified representing 19,287 genes. Comparison of the transcripts in normal and tumor tissue revealed 156 differentially expressed genes (P < 0.05), of which 88 genes were up-regulated and 68 genes were down-regulated in the tumor tissue. Based on SAGE data, we estimate that the transcriptome for human prostate is approximately 37,000. Several differentially expressed genes identified by SAGE were selected for confirmation using immunohistochemistry. Some genes (e.g., E2F4) were overexpressed in tumor epithelial cells and some (e.g., Daxx) were increased in tumor stroma. Further characterization of the role of E2F4 and Daxx as well as other differentially expressed genes may provide useful insights into the mechanism of prostate cancer development.

This article has been cited by other articles:

				J. H. Cho-Vega, S. Tsavachidis, K.-A. Do, J. Nakagawa, L. J. Medeiros, and T. J. McDonnell Dicarbonyl/L-Xylulose Reductase: A Potential Biomarker Identified by Laser-Capture Microdissection-Micro Serial Analysis of Gene Expression of Human Prostate Adenocarcinoma Cancer Epidemiol. Biomarkers Prev., December 1, 2007; 16(12): 2615 - 2622. [Abstract] [Full Text] [PDF]

				L. Bianchetti, Y. Wu, E. Guerin, F. Plewniak, and O. Poch SAGETTARIUS: a program to reduce the number of tags mapped to multiple transcripts and to plan SAGE sequencing stages Nucleic Acids Res., September 25, 2007; 35(18): e122 - e122. [Abstract] [Full Text] [PDF]

				M. Gross, I. Top, I. Laux, J. Katz, J. Curran, C. Tindell, and D. Agus {beta}-2-Microglobulin Is an Androgen-Regulated Secreted Protein Elevated in Serum of Patients with Advanced Prostate Cancer Clin. Cancer Res., April 1, 2007; 13(7): 1979 - 1986. [Abstract] [Full Text] [PDF]

				W. Xu, L. Ngo, G. Perez, M. Dokmanovic, and P. A. Marks Intrinsic apoptotic and thioredoxin pathways in human prostate cancer cell response to histone deacetylase inhibitor PNAS, October 17, 2006; 103(42): 15540 - 15545. [Abstract] [Full Text] [PDF]

				I. M. Berquin, Y. Min, R. Wu, H. Wu, and Y. Q. Chen Expression Signature of the Mouse Prostate J. Biol. Chem., October 28, 2005; 280(43): 36442 - 36451. [Abstract] [Full Text] [PDF]

				D.-Y. Lin, H.-I Fang, A.-H. Ma, Y.-S. Huang, Y.-S. Pu, G. Jenster, H.-J. Kung, and H.-M. Shih Negative Modulation of Androgen Receptor Transcriptional Activity by Daxx Mol. Cell. Biol., December 15, 2004; 24(24): 10529 - 10541. [Abstract] [Full Text] [PDF]

				R. Foley, D. Hollywood, and M. Lawler Molecular pathology of prostate cancer: the key to identifying new biomarkers of disease Endocr. Relat. Cancer, September 1, 2004; 11(3): 477 - 488. [Abstract] [Full Text] [PDF]

				E. L. DuPree, S. Mazumder, and A. Almasan Genotoxic Stress Induces Expression of E2F4, Leading to Its Association with p130 in Prostate Carcinoma Cells Cancer Res., July 1, 2004; 64(13): 4390 - 4393. [Abstract] [Full Text] [PDF]

				L.-Y. Chen and J. D. Chen Daxx Silencing Sensitizes Cells to Multiple Apoptotic Pathways Mol. Cell. Biol., October 15, 2003; 23(20): 7108 - 7121. [Abstract] [Full Text] [PDF]

				W.A. Schulz, M. Burchardt, and M.V. Cronauer Molecular biology of prostate cancer Mol. Hum. Reprod., August 1, 2003; 9(8): 437 - 448. [Abstract] [Full Text] [PDF]

				E. D. Pleasance, M. A. Marra, and S. J.M. Jones Assessment of SAGE in Transcript Identification Genome Res., June 1, 2003; 13(6): 1203 - 1215. [Abstract] [Full Text] [PDF]

				J. L. Dennis, J. K. Vass, E. C. Wit, W. N. Keith, and K. A. Oien Identification from Public Data of Molecular Markers of Adenocarcinoma Characteristic of the Site of Origin Cancer Res., November 1, 2002; 62(21): 5999 - 6005. [Abstract] [Full Text] [PDF]

				G. Untergasser, H. B. Koch, A. Menssen, and H. Hermeking Characterization of Epithelial Senescence by Serial Analysis of Gene Expression: Identification of Genes Potentially Involved in Prostate Cancer Cancer Res., November 1, 2002; 62(21): 6255 - 6262. [Abstract] [Full Text] [PDF]

				E. LaTulippe, J. Satagopan, A. Smith, H. Scher, P. Scardino, V. Reuter, and W. L. Gerald Comprehensive Gene Expression Analysis of Prostate Cancer Reveals Distinct Transcriptional Programs Associated with Metastatic Disease Cancer Res., August 1, 2002; 62(15): 4499 - 4506. [Abstract] [Full Text] [PDF]

				Y. W. Asmann, F. Kosari, K. Wang, J. C. Cheville, and G. Vasmatzis Identification of Differentially Expressed Genes in Normal and Malignant Prostate by Electronic Profiling of Expressed Sequence Tags Cancer Res., June 1, 2002; 62(11): 3308 - 3314. [Abstract] [Full Text] [PDF]

				A. Waghray, D. Keppler, B. F. Sloane, L. Schuger, and Y. Q. Chen Analysis of a Truncated Form of Cathepsin H in Human Prostate Tumor Cells J. Biol. Chem., March 22, 2002; 277(13): 11533 - 11538. [Abstract] [Full Text] [PDF]

				X.-R. Xu, J. Huang, Z.-G. Xu, B.-Z. Qian, Z.-D. Zhu, Q. Yan, T. Cai, X. Zhang, H.-S. Xiao, J. Qu, et al. Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver PNAS, December 18, 2001; 98(26): 15089 - 15094. [Abstract] [Full Text] [PDF]