This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ko, Y. Articles by Brüning, T. Search for Related Content PubMed PubMed Citation Articles by Ko, Y. Articles by Brüning, T.

Association of CYP1B1 Codon 432 Mutant Allele in Head and Neck Squamous Cell Cancer Is Reflected by Somatic Mutations of p53 in Tumor Tissue

Department of Internal Medicine (Medizinische Universitäts-Poliklinik), University of Bonn, 53111 Bonn [Y. K., V. H., C. A., M. E. O-P., H. V.]; Medical Institute of Environmental Hygiene at the Heinrich-Heine-University of Düsseldorf, Department of Experimental Toxicology, 40225 Düsseldorf [J. A., S. D.]; Department of Oto-Rhino-Laryngology [C. H.] and Institute of Pathology [H-P. F.], University of Bonn, 53011 Bonn; Institute for Occupational Physiology at the University of Dortmund, 44139 Dortmund [H-M. B., R. T., P. B.]; Institute of Neurophysiology University of Cologne, 50931 Köln [A. S.]; and Berufsgenossenschaftliches Forschungsinstitut für Arbeitsmedizin (BGFA), 44789 Bochum [T. B.], Germany

Tobacco use is causally associated with head and neck squamous cell cancer (HNSCC). Here, we present the results of a case-control study that investigated the effects that the genetic variants of the cytochrome (CYP)1A1, CYP1B1, glutathione-S-transferase (GST)M1, GSTT1, and GSTP1 genes have on modifying the risk of smoking-related HNSCC. Allelisms of the CYP1A1, GSTT1, GSTM1, and GSTT1 genes alone were not associated with an increased risk. CYP1B1 codon 432 polymorphism was found to be a putative susceptibility factor in smoking-related HNSCC. The frequency of CYP1B1 polymorphism was significantly higher (P < 0.001) in the group of smoking cases when compared with smoking controls. Additionally, an odds ratio (OR) of 4.53 (2.62–7.98) was discovered when investigating smoking and nonsmoking cases for the susceptible genotype CYP1B1*2/*2, when compared with the presence of the genotype wild type. In combination with polymorphic variants of the GST genes, a synergistic-effect OR was observed. The calculated OR for the combined genotype CYP1B1*2/*2 and GSTM1*2/*2 was 12.8 (4.09–49.7). The calculated OR for the combined genotype was 13.4 (2.92–97.7) for CYP1B1*2/*2 and GSTT1*2/*2, and 24.1 (9.36–70.5) for the combination of CYP1B1*2/*2 and GSTT1-expressors. The impact of the polymorphic variants of the CYP1B1 gene on HNSCC risk is reflected by the strong association with the frequency of somatic mutations of the p53 gene. Smokers with susceptible genotype CYP1B1*2/*2 were 20 times more likely to show evidence of p53 mutations than were those with CYP1B1 wild type. Combined genotype analysis of CYP1B1 and GSTM1 or GSTT1 revealed interactive effects on the occurrence of p53 gene mutations. The results of the present study indicate that polymorphic variants of CYP1B1 relate significantly to the individual susceptibility of smokers to HNSCC.

This article has been cited by other articles:

				S. Helmig, B. Hadzaad, J. Dohrel, and J. Schneider Influence of the Cyp1B1 L432V Gene Polymorphism and Exposure to Tobacco Smoke on Cyp1B1 mRNA Expression in Human Leukocytes Drug Metab. Dispos., July 1, 2009; 37(7): 1490 - 1495. [Abstract] [Full Text] [PDF]

				Z. H. Gumus, B. Du, A. Kacker, J. O. Boyle, J. M. Bocker, P. Mukherjee, K. Subbaramaiah, A. J. Dannenberg, and H. Weinstein Effects of Tobacco Smoke on Gene Expression and Cellular Pathways in a Cellular Model of Oral Leukoplakia Cancer Prevention Research, July 1, 2008; 1(2): 100 - 111. [Abstract] [Full Text] [PDF]

				F. Chitsazian, B. K. Tusi, E. Elahi, H. A. Saroei, M. H. Sanati, S. Yazdani, M. Pakravan, N. Nilforooshan, Y. Eslami, M. A. Z. Mehrjerdi, et al. CYP1B1 Mutation Profile of Iranian Primary Congenital Glaucoma Patients and Associated Haplotypes J. Mol. Diagn., July 1, 2007; 9(3): 382 - 393. [Abstract] [Full Text] [PDF]

				E. Aklillu, S. Ovrebo, I. V. Botnen, C. Otter, and M. Ingelman-Sundberg Characterization of Common CYP1B1 Variants with Different Capacity for Benzo[a]pyrene-7,8-Dihydrodiol Epoxide Formation from Benzo[a]pyrene Cancer Res., June 15, 2005; 65(12): 5105 - 5111. [Abstract] [Full Text] [PDF]

				L. F. Masson, L. Sharp, S. C. Cotton, and J. Little Cytochrome P-450 1A1 Gene Polymorphisms and Risk of Breast Cancer: A HuGE Review Am. J. Epidemiol., May 15, 2005; 161(10): 901 - 915. [Abstract] [Full Text] [PDF]

				G. Li, Z. Liu, E. M. Sturgis, R. M. Chamberlain, M. R. Spitz, and Q. Wei CYP2E1 G1532C, NQO1 Pro187Ser, and CYP1B1 Val432Leu Polymorphisms Are Not Associated with Risk of Squamous Cell Carcinoma of the Head and Neck Cancer Epidemiol. Biomarkers Prev., April 1, 2005; 14(4): 1034 - 1036. [Full Text] [PDF]

				S. Bandiera, S. Weidlich, V. Harth, P. Broede, Y. Ko, and T. Friedberg Proteasomal Degradation of Human CYP1B1: Effect of the Asn453Ser Polymorphism on the Post-Translational Regulation of CYP1B1 Expression Mol. Pharmacol., February 1, 2005; 67(2): 435 - 443. [Abstract] [Full Text] [PDF]

				J. L. Port, K. Yamaguchi, B. Du, M. De Lorenzo, M. Chang, P. M. Heerdt, L. Kopelovich, C. B. Marcus, N. K. Altorki, K. Subbaramaiah, et al. Tobacco smoke induces CYP1B1 in the aerodigestive tract Carcinogenesis, November 1, 2004; 25(11): 2275 - 2281. [Abstract] [Full Text] [PDF]

				Z Ye, H Song, and Y Guo Glutathione S-transferase M1, T1 status and the risk of head and neck cancer: a meta-analysis J. Med. Genet., May 1, 2004; 41(5): 360 - 365. [Full Text] [PDF]

				T. Almahmeed, J. O. Boyle, E. G. Cohen, J. F. Carew, B. Du, N. K. Altorki, L. Kopelovich, J.-L. Fang, P. Lazarus, K. Subbaramaiah, et al. Benzo[a]pyrene phenols are more potent inducers of CYP1A1, CYP1B1 and COX-2 than benzo[a]pyrene glucuronides in cell lines derived from the human aerodigestive tract Carcinogenesis, May 1, 2004; 25(5): 793 - 799. [Full Text] [PDF]

				M. Hashibe, P. Brennan, R. C. Strange, R. Bhisey, I. Cascorbi, P. Lazarus, M. B. O. Ophuis, S. Benhamou, W. D. Foulkes, T. Katoh, et al. Meta- and Pooled Analyses of GSTM1, GSTT1, GSTP1, and CYP1A1 Genotypes and Risk of Head and Neck Cancer Cancer Epidemiol. Biomarkers Prev., December 1, 2003; 12(12): 1509 - 1517. [Abstract] [Full Text] [PDF]

				J. S. Mammen, G. S. Pittman, Y. Li, F. Abou-Zahr, B. A. Bejjani, D. A. Bell, P. T. Strickland, and T. R. Sutter Single amino acid mutations, but not common polymorphisms, decrease the activity of CYP1B1 against (-)benzo[a]pyrene-7R-trans-7,8-dihydrodiol Carcinogenesis, July 1, 2003; 24(7): 1247 - 1255. [Abstract] [Full Text] [PDF]

				I. R. Stoilov, V. P. Costa, J. P. C. Vasconcellos, M. B. Melo, A. J. Betinjane, J. C. E. Carani, E. V. Oltrogge, and M. Sarfarazi Molecular Genetics of Primary Congenital Glaucoma in Brazil Invest. Ophthalmol. Vis. Sci., June 1, 2002; 43(6): 1820 - 1827. [Abstract] [Full Text] [PDF]