| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Advances in Brief |
Department of Experimental Oncology [G. S., D. C., L. R., M. A. P.], Department of Statistics [L. M., S. L. V.], Unit of Immunohematology [C. L.], Unit of Thoracic Surgery [L. T.], Istituto Nazionale Tumori, 20133 Milan, Italy
We evaluated whether the amount of circulating DNA in plasma could discriminate between lung cancer patients and healthy individuals and whether it is related to disease progression, and we analyzed the kinetics of plasma DNA in disease-free, surgically resected patients. Plasma DNA quantification and analysis of microsatellite alterations were performed in a consecutive series of 84 patients with non-small cell lung cancer, who were studied during follow-up, and 43 healthy controls. In patients, the mean values of plasma DNA concentration were higher than in controls even considering stage Ia patients. Sensitivity and specificity estimates were calculated as the area under the receiver operating characteristic curve (AUC-ROC) curve and showed a value of 0.844. Variations in DNA level and in microsatellite changes correlated with the clinical status of 38 patients monitored during follow-up. The data suggest that quantification and molecular characterization of plasma DNA in lung cancer patients are valuable noninvasive diagnostic tools for discriminating patients from unaffected individuals and for detecting early recurrence during follow-up.
This article has been cited by other articles:
![]() |
H. Goldshtein, M. J Hausmann, and A. Douvdevani A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids Ann Clin Biochem, November 1, 2009; 46(6): 488 - 494. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Hohaus, M. Giachelia, G. Massini, G. Mansueto, B. Vannata, V. Bozzoli, M. Criscuolo, F. D'Alo, M. Martini, L. M. Larocca, et al. Cell-free circulating DNA in Hodgkin's and non-Hodgkin's lymphomas Ann. Onc., August 1, 2009; 20(8): 1408 - 1413. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Verri, L. Roz, D. Conte, T. Liloglou, A. Livio, A. Vesin, A. Fabbri, F. Andriani, C. Brambilla, L. Tavecchio, et al. Fragile Histidine Triad Gene Inactivation in Lung Cancer: The European Early Lung Cancer Project Am. J. Respir. Crit. Care Med., March 1, 2009; 179(5): 396 - 401. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Sozzi, L. Roz, D. Conte, L. Mariani, F. Andriani, S. Lo Vullo, C. Verri, and U. Pastorino Plasma DNA Quantification in Lung Cancer Computed Tomography Screening: Five-Year Results of a Prospective Study Am. J. Respir. Crit. Care Med., January 1, 2009; 179(1): 69 - 74. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Holdenrieder, J. von Pawel, E. Dankelmann, T. Duell, B. Faderl, A. Markus, M. Siakavara, H. Wagner, K. Feldmann, H. Hoffmann, et al. Nucleosomes, ProGRP, NSE, CYFRA 21-1, and CEA in Monitoring First-Line Chemotherapy of Small Cell Lung Cancer Clin. Cancer Res., December 1, 2008; 14(23): 7813 - 7821. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Terrin, E. Rampazzo, S. Pucciarelli, M. Agostini, R. Bertorelle, G. Esposito, P. DelBianco, D. Nitti, and A. De Rossi Relationship Between Tumor and Plasma Levels of hTERT mRNA in Patients with Colorectal Cancer: Implications for Monitoring of Neoplastic Disease Clin. Cancer Res., November 15, 2008; 14(22): 7444 - 7451. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. Yagyu, T. Gotoh, T. Iehara, M. Miyachi, Y. Katsumi, S. Tsubai-Shimizu, K. Kikuchi, S. Tamura, K. Tsuchiya, T. Imamura, et al. Circulating Methylated-DCR2 Gene in Serum as an Indicator of Prognosis and Therapeutic Efficacy in Patients with MYCN Nonamplified Neuroblastoma Clin. Cancer Res., November 1, 2008; 14(21): 7011 - 7019. [Abstract] [Full Text] [PDF] |
||||
![]() |
C.-H. Yeh, W. A. Whitmire III, and M. Albitar Liquid-Based Fluorescence In Situ Hybridization Assay for Detection of ERBB2 Gene Amplification in Patients with Breast Cancer Clin. Chem., November 1, 2008; 54(11): 1831 - 1839. [Abstract] [Full Text] [PDF] |
||||
![]() |
H Zhang, Q Zhao, Y Chen, Y Wang, S Gao, Y Mao, M Li, A Peng, D He, and X Xiao Selective expression of S100A7 in lung squamous cell carcinomas and large cell carcinomas but not in adenocarcinomas and small cell carcinomas Thorax, April 1, 2008; 63(4): 352 - 359. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. E. Carpagnano, M. P. Foschino-Barbaro, A. Spanevello, O. Resta, F. Carpagnano, G. Mule, R. Pinto, S. Tommasi, and A. Paradiso 3p Microsatellite Signature in Exhaled Breath Condensate and Tumor Tissue of Patients with Lung Cancer Am. J. Respir. Crit. Care Med., February 1, 2008; 177(3): 337 - 341. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Mehra, M. Penning, J. Maas, N. van Daal, R. H. Giles, and E. E. Voest Circulating Mitochondrial Nucleic Acids Have Prognostic Value for Survival in Patients with Advanced Prostate Cancer Clin. Cancer Res., January 15, 2007; 13(2): 421 - 426. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. Flamini, L. Mercatali, O. Nanni, D. Calistri, R. Nunziatini, W. Zoli, P. Rosetti, N. Gardini, A. Lattuneddu, G. M. Verdecchia, et al. Free DNA and Carcinoembryonic Antigen Serum Levels: An Important Combination for Diagnosis of Colorectal Cancer Clin. Cancer Res., December 1, 2006; 12(23): 6985 - 6988. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. K. Pathak, M. Bhutani, S. Kumar, A. Mohan, and R. Guleria Circulating Cell-Free DNA in Plasma/Serum of Lung Cancer Patients as a Potential Screening and Prognostic Tool Clin. Chem., October 1, 2006; 52(10): 1833 - 1842. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. Hagiwara, L. E. Mechanic, G. E. Trivers, H. L. Cawley, M. Taga, E. D. Bowman, K. Kumamoto, P. He, M. Bernard, S. Doja, et al. Quantitative Detection of p53 Mutations in Plasma DNA from Tobacco Smokers Cancer Res., August 15, 2006; 66(16): 8309 - 8317. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Sozzi, L. Roz, D. Conte, L. Mariani, F. Andriani, P. Verderio, and U. Pastorino Effects of Prolonged Storage of Whole Plasma or Isolated Plasma DNA on the Results of Circulating DNA Quantification Assays J Natl Cancer Inst, December 21, 2005; 97(24): 1848 - 1850. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Diehl, M. Li, D. Dressman, Y. He, D. Shen, S. Szabo, L. A. Diaz Jr, S. N. Goodman, K. A. David, H. Juhl, et al. Detection and quantification of mutations in the plasma of patients with colorectal tumors PNAS, November 8, 2005; 102(45): 16368 - 16373. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Woenckhaus, U. Grepmeier, B. Werner, C. Schulz, F. Rockmann, P. J. Wild, G. Rockelein, H. Blaszyk, M. Schuierer, F. Hofstaedter, et al. Microsatellite Analysis of Pleural Supernatants Could Increase Sensitivity of Pleural Fluid Cytology J. Mol. Diagn., October 1, 2005; 7(4): 517 - 524. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. E. Carpagnano, M. P. Foschino-Barbaro, G. Mule, O. Resta, S. Tommasi, A. Mangia, F. Carpagnano, G. Stea, A. Susca, G. Di Gioia, et al. 3p Microsatellite Alterations in Exhaled Breath Condensate from Patients with Non-Small Cell Lung Cancer Am. J. Respir. Crit. Care Med., September 15, 2005; 172(6): 738 - 744. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Gotoh, H. Hosoi, T. Iehara, Y. Kuwahara, S. Osone, K. Tsuchiya, M. Ohira, A. Nakagawara, H. Kuroda, and T. Sugimoto Prediction of MYCN Amplification in Neuroblastoma Using Serum DNA and Real-Time Quantitative Polymerase Chain Reaction J. Clin. Oncol., August 1, 2005; 23(22): 5205 - 5210. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. N. Tamkovich, O. E. Bryzgunova, E. Yu. Rykova, V. I. Permyakova, V. V. Vlassov, and P. P. Laktionov Circulating Nucleic Acids in Blood of Healthy Male and Female Donors Clin. Chem., July 1, 2005; 51(7): 1317 - 1319. [Full Text] [PDF] |
||||
![]() |
M. Frattini, D. Balestra, P. Verderio, G. Gallino, E. Leo, G. Sozzi, M. A. Pierotti, and M. G. Daidone Reproducibility of a Semiquantitative Measurement of Circulating DNA in Plasma From Neoplastic Patients J. Clin. Oncol., May 1, 2005; 23(13): 3163 - 3164. [Full Text] [PDF] |
||||
![]() |
G. Sozzi, D. Conte, and L. Roz In Reply: J. Clin. Oncol., May 1, 2005; 23(13): 3164 - 3165. [Full Text] [PDF] |
||||
![]() |
J. L. Boddy, S. Gal, P. R. Malone, A. L. Harris, and J. S. Wainscoat Prospective Study of Quantitation of Plasma DNA Levels in the Diagnosis of Malignant versus Benign Prostate Disease Clin. Cancer Res., February 15, 2005; 11(4): 1394 - 1399. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. I.L. Sjoholm, G. Hoffmann, S. Lindgren, J. Dillner, and J. Carlson Comparison of Archival Plasma and Formalin-Fixed Paraffin-Embedded Tissue for Genotyping in Hepatocellular Carcinoma Cancer Epidemiol. Biomarkers Prev., January 1, 2005; 14(1): 251 - 255. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. J. Herrera, S. Raja, W. E. Gooding, T. El-Hefnawy, L. Kelly, J. D. Luketich, and T. E. Godfrey Quantitative Analysis of Circulating Plasma DNA as a Tumor Marker in Thoracic Malignancies Clin. Chem., January 1, 2005; 51(1): 113 - 118. [Abstract] [Full Text] [PDF] |
||||
![]() |
O. Gautschi, C. Bigosch, B. Huegli, M. Jermann, A. Marx, E. Chasse, D. Ratschiller, W. Weder, M. Joerger, D. C. Betticher, et al. Circulating Deoxyribonucleic Acid As Prognostic Marker in Non-Small-Cell Lung Cancer Patients Undergoing Chemotherapy J. Clin. Oncol., October 15, 2004; 22(20): 4157 - 4164. [Abstract] [Full Text] [PDF] |
||||
![]() |
T.-S. Wong, D. L.-W. Kwong, J. S.-T. Sham, W. I. Wei, Y.-L. Kwong, and A. P.-W. Yuen Quantitative Plasma Hypermethylated DNA Markers of Undifferentiated Nasopharyngeal Carcinoma Clin. Cancer Res., April 1, 2004; 10(7): 2401 - 2406. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. E. Cottrell, J. Distler, N. S. Goodman, S. H. Mooney, A. Kluth, A. Olek, I. Schwope, R. Tetzner, H. Ziebarth, and K. Berlin A real-time PCR assay for DNA-methylation using methylation-specific blockers Nucleic Acids Res., January 13, 2004; 32(1): e10 - e10. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Sozzi, D. Conte, M. Leon, R. Cirincione, L. Roz, C. Ratcliffe, E. Roz, N. Cirenei, M. Bellomi, G. Pelosi, et al. Quantification of Free Circulating DNA As a Diagnostic Marker in Lung Cancer J. Clin. Oncol., November 1, 2003; 21(21): 3902 - 3908. [Abstract] [Full Text] [PDF] |
||||
![]() |
P. A. Bunn Jr Early Detection of Lung Cancer Using Serum RNA or DNA Markers: Ready for """Prime Time""" or for Validation? J. Clin. Oncol., November 1, 2003; 21(21): 3891 - 3893. [Full Text] [PDF] |
||||
![]() |
C. Stemmer, M. Beau-Faller, E. Pencreac'h, E. Guerin, A. Schneider, D. Jaqmin, E. Quoix, M.-P. Gaub, and P. Oudet Use of Magnetic Beads for Plasma Cell-free DNA Extraction: Toward Automation of Plasma DNA Analysis for Molecular Diagnostics Clin. Chem., November 1, 2003; 49(11): 1953 - 1955. [Full Text] [PDF] |
||||
![]() |
D. W. Swinkels, E. Wiegerinck, E. A.P. Steegers, and J. B. de Kok Effects of Blood-Processing Protocols on Cell-free DNA Quantification in Plasma Clin. Chem., March 1, 2003; 49(3): 525 - 526. [Full Text] [PDF] |
||||
![]() |
J. M. Silva, J. Silva, A. Sanchez, J. M. Garcia, G. Dominguez, M. Provencio, L. Sanfrutos, E. Jareno, A. Colas, P. Espana, et al. Tumor DNA in Plasma at Diagnosis of Breast Cancer Patients Is a Valuable Predictor of Disease-free Survival Clin. Cancer Res., December 1, 2002; 8(12): 3761 - 3766. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. Bearzatto, D. Conte, M. Frattini, N. Zaffaroni, F. Andriani, D. Balestra, L. Tavecchio, M. G. Daidone, and G. Sozzi p16INK4A Hypermethylation Detected by Fluorescent Methylation-specific PCR in Plasmas from Non-Small Cell Lung Cancer Clin. Cancer Res., December 1, 2002; 8(12): 3782 - 3787. [Abstract] [Full Text] [PDF] |
||||
![]() |
G. Dominguez, J. Carballido, J. Silva, J. M. Silva, J. M. Garcia, J. Menendez, M. Provencio, P. Espana, and F. Bonilla p14ARF Promoter Hypermethylation in Plasma DNA as an Indicator of Disease Recurrence in Bladder Cancer Patients Clin. Cancer Res., April 1, 2002; 8(4): 980 - 985. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |