This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yang, X. Articles by Lippman, M. E. Search for Related Content PubMed PubMed Citation Articles by Yang, X. Articles by Lippman, M. E.

Overexpression of KAI1 Suppresses in Vitro Invasiveness and in Vivo Metastasis in Breast Cancer Cells¹

Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC 20007

KAI1 is a metastasis suppressor gene for human prostate cancer and is also involved in the progression of a variety of other human cancers. Previously, we have demonstrated that KAI1 expression was down-regulated in metastatic breast cancer cell lines as well as in highly aggressive breast cancer specimens. To determine whether KAI1 expression is responsible for the metastasis suppression in breast cancer, we transfected the human KAI1 cDNA into two highly malignant breast cancer cell lines, LCC6 and MDA-MB-231, which both have low levels of endogenous KAI1 expression. Parental, vector-only transfectants and KAI1 transfectant clones were injected into the mammary fat pads and tail veins, respectively, of athymic nude mice and assessed for both spontaneous and experimental lung metastasis. High KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LCC6 cells. Metastasis suppression correlated with the reduced rate of tumor growth and a decreased clonogenicity in soft agar. Furthermore, KAI1 expression significantly suppressed the in vitro cell invasion in KAI1-transfected MDA-MB-231 cells. Our results suggested that KAI1 may function as a negative regulator of breast cancer metastasis.

This article has been cited by other articles:

				A. Mela and J. E. Goldman The Tetraspanin KAI1/CD82 Is Expressed by Late-Lineage Oligodendrocyte Precursors and May Function to Restrict Precursor Migration and Promote Oligodendrocyte Differentiation and Myelination J. Neurosci., September 9, 2009; 29(36): 11172 - 11181. [Abstract] [Full Text] [PDF]

				R. Sadej, H. Romanska, G. Baldwin, K. Gkirtzimanaki, V. Novitskaya, A. D. Filer, Z. Krcova, R. Kusinska, J. Ehrmann, C. D. Buckley, et al. CD151 Regulates Tumorigenesis by Modulating the Communication between Tumor Cells and Endothelium Mol. Cancer Res., June 1, 2009; 7(6): 787 - 798. [Abstract] [Full Text] [PDF]

				R. Bari, Y. H. Zhang, F. Zhang, N. X. Wang, C. S. Stipp, J. J. Zheng, and X. A. Zhang Transmembrane Interactions Are Needed for KAI1/CD82-Mediated Suppression of Cancer Invasion and Metastasis Am. J. Pathol., February 1, 2009; 174(2): 647 - 660. [Abstract] [Full Text] [PDF]

				T. Lekishvili, E. Fromm, M. Mujoomdar, and F. Berditchevski The tumour-associated antigen L6 (L6-Ag) is recruited to the tetraspanin-enriched microdomains: implication for tumour cell motility J. Cell Sci., March 1, 2008; 121(5): 685 - 694. [Abstract] [Full Text] [PDF]

				H. Huang, J. Groth, K. Sossey-Alaoui, L. Hawthorn, S. Beall, and J. Geradts Aberrant Expression of Novel and Previously Described Cell Membrane Markers in Human Breast Cancer Cell Lines and Tumors Clin. Cancer Res., June 15, 2005; 11(12): 4357 - 4364. [Abstract] [Full Text] [PDF]

				R. Bass, F. Werner, E. Odintsova, T. Sugiura, F. Berditchevski, and V. Ellis Regulation of Urokinase Receptor Proteolytic Function by the Tetraspanin CD82 J. Biol. Chem., April 15, 2005; 280(15): 14811 - 14818. [Abstract] [Full Text] [PDF]

				B. He, L. Liu, G. A. Cook, S. Grgurevich, L. K. Jennings, and X. A. Zhang Tetraspanin CD82 Attenuates Cellular Morphogenesis through Down-regulating Integrin {alpha}6-Mediated Cell Adhesion J. Biol. Chem., February 4, 2005; 280(5): 3346 - 3354. [Abstract] [Full Text] [PDF]

				B. Zhou, L. Liu, M. Reddivari, and X. A. Zhang The Palmitoylation of Metastasis Suppressor KAI1/CD82 Is Important for Its Motility- and Invasiveness-Inhibitory Activity Cancer Res., October 15, 2004; 64(20): 7455 - 7463. [Abstract] [Full Text] [PDF]

				J. H. Lee, S. R. Park, K.-O. Chay, Y.-W. Seo, H. Kook, K. Y. Ahn, Y. J. Kim, and K. K. Kim KAI1 COOH-Terminal Interacting Tetraspanin (KITENIN), a Member of the Tetraspanin Family, Interacts with KAI1, a Tumor Metastasis Suppressor, and Enhances Metastasis of Cancer Cancer Res., June 15, 2004; 64(12): 4235 - 4243. [Abstract] [Full Text] [PDF]

				M. Kawasaki, T. Maeda, K. Hanasawa, I. Ohkubo, and T. Tani Effect of His-Gly-Lys Motif Derived from Domain 5 of High Molecular Weight Kininogen on Suppression of Cancer Metastasis Both in Vitro and in Vivo J. Biol. Chem., December 5, 2003; 278(49): 49301 - 49307. [Abstract] [Full Text] [PDF]

				X. A. Zhang, B. He, B. Zhou, and L. Liu Requirement of the p130CAS-Crk Coupling for Metastasis Suppressor KAI1/CD82-mediated Inhibition of Cell Migration J. Biol. Chem., July 11, 2003; 278(29): 27319 - 27328. [Abstract] [Full Text] [PDF]

				X. A. Zhang, W. S. Lane, S. Charrin, E. Rubinstein, and L. Liu EWI2/PGRL Associates with the Metastasis Suppressor KAI1/CD82 and Inhibits the Migration of Prostate Cancer Cells Cancer Res., May 15, 2003; 63(10): 2665 - 2674. [Abstract] [Full Text] [PDF]

				K. S. Wilson, H. Roberts, R. Leek, A. L. Harris, and J. Geradts Differential Gene Expression Patterns in HER2/neu-Positive and -Negative Breast Cancer Cell Lines and Tissues Am. J. Pathol., October 1, 2002; 161(4): 1171 - 1185. [Abstract] [Full Text] [PDF]

				K. Uzawa, K. Ono, H. Suzuki, C. Tanaka, T. Yakushiji, N. Yamamoto, H. Yokoe, and H. Tanzawa High Prevalence of Decreased Expression of KAI1 Metastasis Suppressor in Human Oral Carcinogenesis Clin. Cancer Res., March 1, 2002; 8(3): 828 - 835. [Abstract] [Full Text] [PDF]