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[Cancer Research 61, 6029-6033, August 15, 2001]
© 2001 American Association for Cancer Research

Advances in Brief

Quantitation of Serum Prostate-specific Membrane Antigen by a Novel Protein Biochip Immunoassay Discriminates Benign from Malignant Prostate Disease1

Zhen Xiao, Bao-Ling Adam, Lisa H. Cazares, Mary Ann Clements, John W. Davis, Paul F. Schellhammer, Enrique A. Dalmasso and George L. Wright, Jr.2

Department of Microbiology and Molecular Cell Biology and Virginia Prostate Center, Eastern Virginia Medical School, Norfolk, Virginia 23507 [Z. X., B-L. A., L. H. C., M. A. C., J. W. D., P. F. S., G. L. W.], and Ciphergen Biosystems, Inc., Fremont, California 94555 [E. A. D.]

The lack of a sensitive immunoassay for quantitating serum prostate-specific membrane antigen (PSMA) hinders its clinical utility as a diagnostic/prognostic biomarker. An innovative protein biochip immunoassay was used to quantitate and compare serum PSMA levels in healthy men and patients with either benign or malignant prostate disease. PSMA was captured from serum by anti-PSMA antibody bound to ProteinChip arrays, the captured PSMA detected by surface-enhanced laser desorption/ionization mass spectrometry, and quantitated by comparing the mass signal integrals to a standard curve established using purified recombinant PSMA. The average serum PSMA value for prostate cancer (623.1 ng/ml) was significantly different (P < 0.001) from that for benign prostate hyperplasia (117.1 ng/ml) and the normal groups (age <50, 272.9 ng/ml; age >50, 359.4 ng/ml). These initial results suggest that serum PSMA may be a more effective biomarker than prostate-specific antigen for differentiating benign from malignant prostate disease and warrants additional evaluation of the surface-enhanced laser desorption/ionization PSMA immunoassay to determine its diagnostic utility.




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Copyright © 2001 by the American Association for Cancer Research.