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Chromosomal Rearrangements and Oncogene Amplification Precede Aneuploidization in the Genetic Evolution of Breast Cancer¹

Department of Oncology, Jubileum Institute, University Hospital, S-221 85 Lund, Sweden [K. R., B. B., M. T., J. I.]; Institute of Medical Technology, Tampere University and University Hospital, Tampere, FIN-33101 Finland [S. K., M. T., J. I.]; and Department of Molecular Medicine, Karolinska Hospital, CMML 8:01, Stockholm, S-17176 Sweden [S. K.]

Breast carcinoma is thought to arise because of multiple successive changes in the genome of the normal epithelial cells. However, little is known of the order of appearance of different types of genetic aberrations. We studied the ERBB2 (Her-2/neu) and CCND1 (cyclin D1) oncogene amplification in flow cytometrically sorted diploid and nondiploid tumor cell populations by fluorescence in situ hybridization (FISH). The purity of the cell sorting was confirmed by static DNA image cytometry. Spectral karyotyping was used to define differences in a genome-wide manner between two distinctly different aneuploid cell clones found in each of two breast cancer cell lines. FISH indicated the presence of gene amplification both in diploid and nondiploid cell clones in 17 of the 21 amplification-containing tumors analyzed. The oncogene copy numbers remained unchanged throughout aneuploidization in 11 of 17 tumors. The remaining six tumors showed an increase in oncogene copy number as well as the number of chromosome 11 or 17 centromeres (the original location of CCND1 and ERBB2, respectively). Breast carcinoma cell lines MDA-157 and MDA-436 showed a significant number of chromosomal rearrangements in the near-diploid clones, which were present in duplicate in the corresponding aneuploid (polyploid) clones. These results indicate that ploidy shift, i.e., aneuploidization, in breast cancer is a late genetic event, which is preceded by both oncogene amplifications as well as many chromosomal rearrangements.

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