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Production, and Expansion of Natural Killer Cells1
Department of Surgery and Division of Biologic Therapeutics, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15261 [Y-I. S., R. M. D., R. B. M., S. E., M. T. L.], and Department of Oncology Research, SmithKline Beecham, King of Prussia, Pennsylvania 19406 [Z. L. J.]
We investigated the in vitro effects of combining
interleukin-18 (IL-18) and IL-2 on human lymphocytes. The combined use
of these two cytokines synergistically enhanced the proliferation,
cytolytic activity, and interferon-
production of peripheral blood
mononuclear cells. Phenotypic analysis revealed a preferential
expansion of CD56+CD3- cells and an
up-regulation of IL-2 receptor-
expression on natural killer cells.
Isolated natural killer cells showed a substantial increase in
proliferation and cytotoxicity compared with CD4+ and
CD8+ T cells. The combined use of IL-18 and IL-2 should be
considered a viable strategy to induce an antitumor response in
vivo.
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