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[Cancer Research 61, 921-925, February 1, 2001]
© 2001 American Association for Cancer Research

Advances in Brief

Wilms’ Tumor Suppressor Gene (WT1) Is Expressed in Primary Breast Tumors Despite Tumor-specific Promoter Methylation1

David M. Loeb, Ella Evron, Chirag B. Patel, Prem Mohini Sharma, Birunthi Niranjan, Lakjaya Buluwela, Sigmund A. Weitzman, Dorian Korz and Saraswati Sukumar2

Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231 [D. M. L., E. E., C. B. P., D. K., S. S.]; Department of Medicine, University of California, San Diego, California 92161 [P. M. S.]; Division of Medicine, Imperial College School of Medicine, United Kingdom W12 0NN [B. N., L. B.]; and Northwestern University Medical School, Chicago, Illinois 60611 [S. A. W.]

We analyzed Wilms’ tumor suppressor 1 (WT1) expression and its regulation by promoter methylation in a panel of normal breast epithelial samples and primary carcinomas. Contrary to previous reports, WT1 protein was strongly expressed in primary carcinomas (27 of 31 tumors) but not in normal breast epithelium (1 of 20 samples). Additionally, the WT1 promoter was methylated in 6 of 19 (32%) primary tumors, which nevertheless expressed WT1. The promoter is not methylated in normal epithelium. Thus, although tumor-specific methylation of WT1 is established in primary breast cancer at a low frequency, other transcriptional regulatory mechanisms appear to supercede its effects in these tumors. Our results demonstrate expression of WT1 in mammary neoplasia, and that WT1 may not have a tumor suppressor role in breast cancer.




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