This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lin, B. Articles by Nelson, P. S. Search for Related Content PubMed PubMed Citation Articles by Lin, B. Articles by Nelson, P. S.

Prostate Short-Chain Dehydrogenase Reductase 1 (PSDR1): A New Member of the Short-Chain Steroid Dehydrogenase/Reductase Family Highly Expressed in Normal and Neoplastic Prostate Epithelium¹

Departments of Molecular Biotechnology [B. L., J. T. W., P. S. N.], Medicine [P. S. N.], Urology [S. W., R. V.], Microbiology [R. B.], and Pathology [L. D. T.], University of Washington, Seattle, Washington 98195; the Institute for Systems Biology, Seattle, Washington 98105 [L. H.]; and the Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 [C. F., P. S. N.]

Genes regulated by androgenic hormones are of critical importance for the normal physiological function of the human prostate gland, and they contribute to the development and progression of prostate carcinoma. We used cDNA microarrays comprised of prostate-derived cDNAs to profile transcripts regulated by androgens in prostate cancer cells. This study identified a novel gene that we have designated prostate short-chain dehydrogenase/reductase 1 (PSDR1), that exhibits increased expression on exposure to androgens in the LNCaP prostate cancer cell line. Northern analysis demonstrated that PSDR1 is highly expressed in the prostate gland relative to other normal human tissues. The PSDR1 cDNA and putative protein exhibit homology to the family of short-chain dehydrogenase/reductase enzymes and thus identify a new member of this family. Cloning and analysis of the putative PSDR1 promoter region identified a potential androgen-response element. We used a radiation-hybrid panel to map the PSDR1 gene to chromosome 14q23-24.3. In situ hybridization localizes PSDR1 expression to normal and neoplastic prostate epithelium. These results identify a new gene involved in the androgen receptor-regulated gene network of the human prostate that may play a role in the pathogenesis of prostate carcinoma.

This article has been cited by other articles:

				M. Liden and U. Eriksson Understanding Retinol Metabolism: Structure and Function of Retinol Dehydrogenases J. Biol. Chem., May 12, 2006; 281(19): 13001 - 13004. [Full Text] [PDF]

				A. Kasus-Jacobi, J. Ou, D. G. Birch, K. G. Locke, J. M. Shelton, J. A. Richardson, A. J. Murphy, D. M. Valenzuela, G. D. Yancopoulos, and A. O. Edwards Functional Characterization of Mouse RDH11 as a Retinol Dehydrogenase Involved in Dark Adaptation in Vivo J. Biol. Chem., May 27, 2005; 280(21): 20413 - 20420. [Abstract] [Full Text] [PDF]

				T. S. Kim, A. Maeda, T. Maeda, C. Heinlein, N. Kedishvili, K. Palczewski, and P. S. Nelson Delayed Dark Adaptation in 11-cis-Retinol Dehydrogenase-deficient Mice: A ROLE OF RDH11 IN VISUAL PROCESSES IN VIVO J. Biol. Chem., March 11, 2005; 280(10): 8694 - 8704. [Abstract] [Full Text] [PDF]

				H. Maeda, S. Nagata, C. D. Wolfgang, G. L. Bratthauer, T. K. Bera, and I. Pastan The T Cell Receptor {gamma} Chain Alternate Reading Frame Protein (TARP), a Prostate-specific Protein Localized in Mitochondria J. Biol. Chem., June 4, 2004; 279(23): 24561 - 24568. [Abstract] [Full Text] [PDF]

				J. Lapointe, C. Li, J. P. Higgins, M. van de Rijn, E. Bair, K. Montgomery, M. Ferrari, L. Egevad, W. Rayford, U. Bergerheim, et al. Gene expression profiling identifies clinically relevant subtypes of prostate cancer PNAS, January 20, 2004; 101(3): 811 - 816. [Abstract] [Full Text] [PDF]

				A. Kasus-Jacobi, J. Ou, Y. K. Bashmakov, J. M. Shelton, J. A. Richardson, J. L. Goldstein, and M. S. Brown Characterization of Mouse Short-chain Aldehyde Reductase (SCALD), an Enzyme Regulated by Sterol Regulatory Element-binding Proteins J. Biol. Chem., August 22, 2003; 278(34): 32380 - 32389. [Abstract] [Full Text] [PDF]

				F. Haeseleer, G.-F. Jang, Y. Imanishi, C. A. G. G. Driessen, M. Matsumura, P. S. Nelson, and K. Palczewski Dual-substrate Specificity Short Chain Retinol Dehydrogenases from the Vertebrate Retina J. Biol. Chem., November 15, 2002; 277(47): 45537 - 45546. [Abstract] [Full Text] [PDF]

				N. Y. Kedishvili, O. V. Chumakova, S. V. Chetyrkin, O. V. Belyaeva, E. A. Lapshina, D. W. Lin, M. Matsumura, and P. S. Nelson Evidence That the Human Gene for Prostate Short-chain Dehydrogenase/Reductase (PSDR1) Encodes a Novel Retinal Reductase (RalR1) J. Biol. Chem., August 2, 2002; 277(32): 28909 - 28915. [Abstract] [Full Text] [PDF]

				Y. Kallberg, U. Oppermann, H. Jornvall, and B. Persson Short-chain dehydrogenase/reductase (SDR) relationships: A large family with eight clusters common to human, animal, and plant genomes Protein Sci., March 1, 2002; 11(3): 636 - 641. [Abstract] [Full Text] [PDF]

				C. C. Pritchard, L. Hsu, J. Delrow, and P. S. Nelson Project normal: Defining normal variance in mouse gene expression PNAS, November 6, 2001; 98(23): 13266 - 13271. [Abstract] [Full Text] [PDF]