This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Suminami, Y. Articles by Kato, H. Search for Related Content PubMed PubMed Citation Articles by Suminami, Y. Articles by Kato, H.

Suppression of a Squamous Cell Carcinoma (SCC)-related Serpin, SCC Antigen, Inhibits Tumor Growth with Increased Intratumor Infiltration of Natural Killer Cells¹

Department of Obstetrics and Gynecology [Y. S., A. M., S. Naw., H. H., F. N., H. K.] and First Department of Pathology [T. G], Yamaguchi University School of Medicine, Ube 755-8505, Japan; Department of Microbiology, Dokkyo University School of Medicine, Mibu 321-0293, Japan [S. Nag.]; and Department of Pediatrics, Harvard Medical School, Children’s Hospital, Boston, Massachusetts 02115-5737 [G. A. S.]

Squamous cell carcinoma (SCC) antigen (SCCA), a member of the ovalbumin serine proteinase inhibitor family, serves as a circulating marker of squamous cell carcinoma (SC). One of the SCCAs, SCCA1, has been suggested to play a role in the attenuation of apoptosis in vitro and in the augmentation of tumor growth in vivo. In the present study, the infection of a SCC cell line (SKG IIIa) with recombinant retrovirus that expressed the antisense SCCA mRNA suppressed expression of SCCA in vitro. Local administration of this retrovirus into tumors by inoculation in nude mice suppressed tumor growth. Treatment of tumor tissue in vivo is also associated with increased numbers of apoptotic tumor cells and large mononuclear cells in the tumor. To test the possible role of SCCA in the infiltration of large mononuclear cells, we analyzed the effect of SCCA1 on migration of natural killer (NK) cells induced by monocyte-chemoattractant protein-1 in vitro. SCCA1 suppressed migration of NK cells completely, and this inhibitory effect was lost by mutation of the reactive site loop of SCCA1. These results suggest that antisense SCCA may suppress the growth of SCC in vivo not only by the augmentation of intracellular apoptosis but also by the increased infiltration of NK cells into the tumor.

This article has been cited by other articles:

				F Calabrese, F Lunardi, C Giacometti, G Marulli, M Gnoato, P Pontisso, M Saetta, M Valente, F Rea, E Perissinotto, et al. Overexpression of squamous cell carcinoma antigen in idiopathic pulmonary fibrosis: clinicopathological correlations Thorax, September 1, 2008; 63(9): 795 - 802. [Abstract] [Full Text] [PDF]

				M Guido, T Roskams, P Pontisso, M Fassan, S N Thung, L Giacomelli, A Sergio, F Farinati, U Cillo, and M Rugge Squamous cell carcinoma antigen in human liver carcinogenesis J. Clin. Pathol., April 1, 2008; 61(4): 445 - 447. [Abstract] [Full Text] [PDF]

				N. Devoogdt, G. Hassanzadeh Ghassabeh, J. Zhang, L. Brys, P. De Baetselier, and H. Revets Secretory leukocyte protease inhibitor promotes the tumorigenic and metastatic potential of cancer cells PNAS, May 13, 2003; 100(10): 5778 - 5782. [Abstract] [Full Text] [PDF]

				M. Gariboldi, B. Peissel, A. Fabbri, A. Saran, D. Zaffaroni, F. S. Falvella, M. Spinola, J.-i. Tanuma, S. Pazzaglia, M. T. Mancuso, et al. SCCA2-like Serpins Mediate Genetic Predisposition to Skin Tumors Cancer Res., April 15, 2003; 63(8): 1871 - 1875. [Abstract] [Full Text] [PDF]

				R. Lin, Y. Nagai, R. Sladek, Y. Bastien, J. Ho, K. Petrecca, G. Sotiropoulou, E. P. Diamandis, T. J. Hudson, and J. H. White Expression Profiling in Squamous Carcinoma Cells Reveals Pleiotropic Effects of Vitamin D3 Analog EB1089 Signaling on Cell Proliferation, Differentiation, and Immune System Regulation Mol. Endocrinol., June 1, 2002; 16(6): 1243 - 1256. [Abstract] [Full Text] [PDF]