This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yonou, H. Articles by Ochiai, A. Search for Related Content PubMed PubMed Citation Articles by Yonou, H. Articles by Ochiai, A.

Establishment of a Novel Species- and Tissue-specific Metastasis Model of Human Prostate Cancer in Humanized Non-Obese Diabetic/Severe Combined Immunodeficient Mice Engrafted with Human Adult Lung and Bone¹

Pathology Division, National Cancer Center Research Institute East [H. Y., T. Y., T. K., N. K., T. Has., A. O.] and Division of Thoracic Oncology, National Cancer Center Hospital East [K. N.], Chiba 277-8577, and Department of Urology, University of the Ryukyus, Okinawa 903-0215 [T. Hat., Y. O.], Japan

Bone is the most common site of metastasis in prostate cancer (PC), and to generate an animal model to investigate the basis of the unique organ tropism of PC cells for bone, we engrafted humanized non-obese diabetic/severe combined immunodeficient (NOD/SCID-hu) mice with human adult bone (HAB) and lung (HAL). Human PC cell lines LNCaP (1 x 10⁷) and PC-3 (5 x 10⁶) were injected into male NOD/SCID-hu mice via the lateral tail vein at 3–4 weeks after implantation. At 8 weeks after the injection, LNCaP and PC-3 cells had metastasized specifically to HAB in 35 and 65%, respectively, of the mice. The tumors formed by LNCaP appeared to be the osteoblastic type, whereas the PC-3 tumors consisted of osteolytic lesions without any surrounding osteogenic response. A feature of experimental metastasis of PC in NOD/SCID-hu mice was its specificity for HAB tissue. Human PC cells had no or very low metastatic potential in regard to implanted HAL, mouse bone, or native mouse bone. These findings indicate that metastasis of PC cells to HAB is both species and tissue specific. The availability of this small animal model could provide a useful tool for identifying and analyzing important features of the human PC metastatic process that cannot be addressed in conventional metastasis models.

This article has been cited by other articles:

				N. Koide, T. Yamada, R. Shibata, T. Mori, M. Fukuma, K. Yamazaki, K. Aiura, M. Shimazu, S. Hirohashi, Y. Nimura, et al. Establishment of Perineural Invasion Models and Analysis of Gene Expression Revealed an Invariant Chain (CD74) as a Possible Molecule Involved in Perineural Invasion in Pancreatic Cancer Clin. Cancer Res., April 15, 2006; 12(8): 2419 - 2426. [Abstract] [Full Text] [PDF]

				S. Miwa, A. Mizokami, E. T. Keller, R. Taichman, J. Zhang, and M. Namiki The Bisphosphonate YM529 Inhibits Osteolytic and Osteoblastic Changes and CXCR-4-Induced Invasion in Prostate Cancer Cancer Res., October 1, 2005; 65(19): 8818 - 8825. [Abstract] [Full Text] [PDF]

				C. Kuperwasser, S. Dessain, B. E. Bierbaum, D. Garnet, K. Sperandio, G. P. Gauvin, S. P. Naber, R. A. Weinberg, and M. Rosenblatt A Mouse Model of Human Breast Cancer Metastasis to Human Bone Cancer Res., July 15, 2005; 65(14): 6130 - 6138. [Abstract] [Full Text] [PDF]

				M. Goya, S. Miyamoto, K. Nagai, Y. Ohki, K. Nakamura, K. Shitara, H. Maeda, T. Sangai, K. Kodama, Y. Endoh, et al. Growth Inhibition of Human Prostate Cancer Cells in Human Adult Bone Implanted into Nonobese Diabetic/Severe Combined Immunodeficient Mice by a Ligand-Specific Antibody to Human Insulin-Like Growth Factors Cancer Res., September 1, 2004; 64(17): 6252 - 6258. [Abstract] [Full Text] [PDF]

				D. R. Gray, W. J. Huss, J. M. Yau, L. E. Durham, E. S. Werdin, W. K. Funkhouser Jr., and G. J. Smith Short-Term Human Prostate Primary Xenografts: An in Vivo Model of Human Prostate Cancer Vasculature and Angiogenesis Cancer Res., March 1, 2004; 64(5): 1712 - 1721. [Abstract] [Full Text] [PDF]

				H. Yonou, N. Kanomata, M. Goya, T. Kamijo, T. Yokose, T. Hasebe, K. Nagai, T. Hatano, Y. Ogawa, and A. Ochiai Osteoprotegerin/Osteoclastogenesis Inhibitory Factor Decreases Human Prostate Cancer Burden in Human Adult Bone Implanted into Nonobese Diabetic/Severe Combined Immunodeficient Mice Cancer Res., May 1, 2003; 63(9): 2096 - 2102. [Abstract] [Full Text] [PDF]

				K. D. Miller, C. J. Sweeney, and G. W. Sledge The Snark is a Boojum: the continuing problem of drug resistance in the antiangiogenic era Ann. Onc., January 1, 2003; 14(1): 20 - 28. [Abstract] [Full Text] [PDF]

				M. J. Bissell, J. L. Beyec, and R. L. Anderson Prostate Cancer in Bone: Importance of Context for Inhibition of Matrix Metalloproteinases J Natl Cancer Inst, January 2, 2002; 94(1): 4 - 5. [Full Text] [PDF]