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[Cancer Research 61, 2239-2249, March 1, 2001]
© 2001 American Association for Cancer Research

Tumor Biology

A Probasin-Large T Antigen Transgenic Mouse Line Develops Prostate Adenocarcinoma and Neuroendocrine Carcinoma with Metastatic Potential1

Naoya Masumori, Tania Z. Thomas, Pierre Chaurand, Tom Case, Manik Paul, Susan Kasper, Richard M. Caprioli, Taiji Tsukamoto, Scott B. Shappell and Robert J. Matusik2

Department of Urologic Surgery [N. M., T. Z. T., T. C., M. P., S. K., R. J. M.], Vanderbilt Prostate Cancer Center [N. M., T. Z. T., T. C., M. P., S. K., S. B. S., R. J. M.], Department of Cell Biology [S. K., R. J. M.], Mass Spectrometry Research Center, Department of Biochemistry [P. C., R. M. C.], Department of Pathology [S. B. S.], and The Vanderbilt-Ingram Cancer Center [S. B. S., R. J. M.], Vanderbilt University Medical Center, Nashville, Tennessee 37232, and Department of Urologic Surgery and Andrology, Sapporo Medical University School of Medicine, 060 Sapporo, Japan [N. M., T. T.]

Neuroendocrine (NE) cells may be involved not only in growth and differentiation of the normal prostate but also in carcinogenesis and progression of prostate adenocarcinoma (Pca), including development of androgen resistance. However, the exact pathophysiology of NE cells in Pca remains poorly understood. Here we describe a transgenic model of Pca with progressive NE differentiation. Seven lines of transgenic mice with the rat prostate-specific large probasin promoter linked to the SV40-large T antigen (Tag) that develop prostatic neoplasia have been established. In this study, one of the seven lines (12T-10) was characterized by examination of 52 mice aged from 2–12 months. With advancing age, low-grade prostatic intraepithelial neoplasia, high-grade prostatic intraepithelial neoplasia, microinvasion, invasive carcinoma, and poorly or undifferentiated carcinoma with NE differentiation appeared in the prostates in sequential order. Whereas Tag is expressed uniformly in prostate epithelium, only an increasing subset of cells in prostatic intraepithelial neoplasia showed NE differentiation by chromogranin immunostaining. Frankly invasive carcinoma developing subsequently showed occasional definitive glandular differentiation (adenocarcinoma) and particularly undifferentiated carcinoma with NE histological features similar to those observed in NE carcinomas in humans. The NE carcinomas occurred in the dorsolateral and ventral lobes and were generally androgen receptor negative. Twenty-one of 32 (66%) mice aged >=6 months and 15 of 17 (88%) mice aged >=9 months developed metastatic tumors, as confirmed by histology and/or Tag immunohistochemistry. Metastases occurred at the later time points, with metastasis to regional lymph nodes, liver, and lung being particularly common. Metastases showed histological features of NE differentiation, as confirmed by chromogranin immunostaining and electron microscopy. An athymic nude mouse that received a s.c. implant of a primary NE tumor developed Tag-positive metastatic tumors with similar NE differentiation. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry identified identical protein profiles between the primary NE tumor and lesions in the extraprostatic organs. Hence, in the 12T-10 large probasin promoter-Tag mouse, high-grade prostatic intraepithelial neoplasia develops progressively greater NE differentiation and progresses to invasive adenocarcinoma and NE carcinoma, with a high percentage of metastases. The predictable progression through these stages will allow testing of therapeutic interventions as well as possible further delineation of the role of NE cells in Pca progression.




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