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Norepinephrine-induced Migration of SW 480 Colon Carcinoma Cells Is Inhibited by ß-Blockers¹

Institute for Immunology, Witten/Herdecke University, 58448 Witten, Germany

ß-Adrenoceptors are highly expressed on SW 480 colon carcinoma cells as was assessed by flow cytometry. We investigated the influence of norepinephrine on the migration of these cells using time-lapse videomicroscopy. Norepinephrine-treatment increased the locomotor activity within the population from 25% spontaneously locomoting cells to 65% locomoting cells. The ß1/2-blocker propranolol but not the ß1-blocker atenolol inhibited this increase. The intracellular signaling solely of norepinephrine-induced locomotion involved protein tyrosine kinase activity, whereas both spontaneous and norepinephrine-induced migration were reduced by inhibiting phospholipase C and protein kinase C activity. In summary, norepinephrine-induced locomotion of SW 480 cells is ß2-adrenoceptor mediated and distinct from spontaneous locomotion concerning the PTK involvement.

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