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Isolation of a Novel Candidate Oncogene within a Frequently Amplified Region at 3q26 in Ovarian Cancer¹

Department of Clinical Oncology, Queen Mary Hospital, The University of Hong Kong [X-Y. G., J. S. T. S., T. C-M. T.]; Cancer Institute, Sun Yat-sen University of Medical Sciences, Guangzhou, People’s Republic of China [F. Y.]; State Key Laboratory of Genetic Engineering Institute of Genetics, Fudan University, Shanghai, People’s Republic of China [K-K. H.]; and Arizona Cancer Center, The University of Arizona, Tucson, Arizona [J-M. Y.]

Amplification of 3q25-q26 was one of the most frequent chromosomal alterations in human ovarian carcinoma. A chromosome microdissection-hybrid selection method was applied to isolate transcribed sequences from a primary ovarian cancer containing high-copy-number amplification of 3q26 using 3q26 band-specific DNAs generated by chromosome microdissection. Using this method, we have isolated a novel candidate oncogene eIF-5A2 (eukaryotic initiation factor 5A2). eIF-5A2 shares 82% identity of amino acid sequence with eIF-5A including the minimum domain needed for eIF-5A maturation by hypusine modification at lysine-50 residue. Amplification and overexpression of eIF-5A2 was frequently detected in primary ovarian cancers and ovarian cancer cell lines. The proliferation-related function of eIF-5A supports that eIF-5A2 is a candidate oncogene related to the development of ovarian cancer.

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